scholarly journals Early Events in the Infection of Soybean (Glycine max L. Merr) by Rhizobium japonicum

1980 ◽  
Vol 66 (6) ◽  
pp. 1027-1031 ◽  
Author(s):  
T. V. Bhuvaneswari ◽  
B. Gillian Turgeon ◽  
Wolfgang D. Bauer
Keyword(s):  
Glycine Max ◽  
Rhizobium Japonicum ◽  
Glycine Max L

EFFECTS OF INOCULATION AND FERTILIZER N LEVELS ON N2 FIXATION AND YIELDS OF SOYBEANS IN ONTARIO

1979 ◽  
Vol 59 (4) ◽  
pp. 1129-1137 ◽  
Author(s):  
ERNEST SEMU ◽  
D. J. HUME
Keyword(s):  
Glycine Max ◽  
Rhizobium Japonicum ◽  
Soil N ◽  
Fertilizer N ◽  
Fertilizer Nitrogen ◽  
Nodule Number ◽  
Glycine Max L ◽  
Yield Responses ◽  
Seed Yields ◽  
Nodule Efficiency

Soybeans (Glycine max (L.) Merrill) often do not give yield responses to added fertilizer nitrogen (N) because high soil N levels inhibit fixation of atmospheric N2. Yield responses to N fertilizer applied at planting usually indicate that N2 fixation is less than optimal. The effects of inoculation with Rhizobium japonicum, and fertilizer N levels, on soybean N2(C2H2) fixation and seed yields in Ontario were investigated in ’ 1976 and 1977. Three locations were used each year, representing areas where soybeans had been grown for many years (Ridgetown), for only a few years (Elora), or not at all (Woodstock). Treatments were (a) Uninoculated + 0 N, (b–e) Inoculated + 0, 50, 100 or 200 kg N/ha. Results indicated that inoculation increased seed yields only when soybeans were introduced into new areas. Fertilizer N applications at planting time did not increase yields in areas where soybeans had been grown several times previously, indicating that N2 fixation could support maximum yields. Nodule number and mass, and N2(C2H2) fixation rates were all decreased by fertilizer N. An increase in nodule efficiency, later in the season, in high N treatments was most marked at Ridgetown.

PRODUCTION PRACTICES FOR EARLY MATURING SOYBEANS IN SOUTHERN ALBERTA

1983 ◽  
Vol 63 (3) ◽  
pp. 641-647 ◽  
Author(s):  
S. DUBETZ ◽  
D. J. MAJOR ◽  
R. J. RENNIE
Keyword(s):  
Glycine Max ◽  
Field Capacity ◽  
Rhizobium Japonicum ◽  
Yield And Quality ◽  
Southern Alberta ◽  
Early Maturing ◽  
Glycine Max L ◽  
Production Practices ◽  
Irrigation Requirements

Four experiments were conducted at two locations in southern Alberta, where no indigenous Rhizobium japonicum existed, to determine the effects of seeding dates, fertilizers, irrigation, and method of inoculant placement on the yield and quality of several early-maturing soybean (Glycine max (L.) Merrill) cultivars. Highest yields were obtained when soybeans were seeded during the first half of May. Irrigation requirements varied between years but yields were similar whether soil moisture was depleted to the 6 × 104-Pa or the 3 × 104-Pa level before irrigating to field capacity. Soybean yields increased substantially from N fertilizer when the inoculant was applied to the seed without a sticker. When granular inoculant was banded with the seed there was no response to N, and the yields of inoculated plants were higher than those of plants that were uninoculated and received up to 160 kg N/ha. When properly inoculated, early-maturing cultivars such as King Grain strain X005 and Maple Presto were capable of maturing and producing seed of acceptable yield and quality in southern Alberta.Key words: Cultivars, fertilizer, irrigation, inoculant, Glycine max (L.) Merrill

Conservation of leghemoglobin heterogeneity and structures in cultivated and wild soybean

1985 ◽  
Vol 63 (11) ◽  
pp. 1951-1956 ◽  
Author(s):  
W. H. Fuchsman ◽  
R. G. Palmer
Keyword(s):  
Glycine Max ◽  
Isoelectric Focusing ◽  
Bradyrhizobium Japonicum ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Rhizobium Japonicum ◽  
Plant Introductions ◽  
Glycine Max L ◽  
Cultivated Soybean ◽  
Selection Of

The leghemoglobins from a genetically diverse selection of 69 cultivated soybean (Glycine max (L.) Merr.) cultivars and plant introductions and 18 wild soybean (Glycine soja Sieb. & Zucc.) plant introductions all consist of the same set of major leghemoglobins (a, c1, c2, c3), as determined by analytical isoelectric focusing. The conservation of both leghemoglobin heterogeneity and also all four major leghemoglobin structures provides strong circumstantial evidence that leghemoglobin heterogeneity is functional. Glycine max and G. soja produced the same leghemoglobins in the presence of Bradyrhizobium japonicum (Kirchner) Jordan and in the presence of fast-growing Rhizobium japonicum.

scholarly journals Especificidade hospedeira e pré-seleção de estirpes de Rhlzobium japonicum para as variedades Santa-Rosa, viçoja e IAC-2 de soja

Bragantia ◽  
1976 ◽  
Vol 35 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Eli Sidney Lopes ◽  
Antônio Roberto Giardini ◽  
Romeu A. S. Kiihl ◽  
Toshio Igue
Keyword(s):  
Glycine Max ◽  
Rhizobium Japonicum ◽  
Glycine Max L

Um experimento foi conduzido em casa de vegetação, em vasos de Leonard com solução nutritiva sem nitrogênio, em condições assépticas, com os objetivos de pré-selecionar treze estirpes de Rhizobium japonicum e verificar o grau e especificidade das variedades santa-rosa, viçoja e IAC-2 (Glycine max (L.) Merril.). Pela observação do nitrogênio total fixado aos 58 dias, verificou-se que as estirpes SMS-64 (= 513 Re, RS), SMS-65 (= 519 Re, RS), SMS-68 (= 532c, RS = PR-G3, IBPT, PR) SMS-309 e SMS-310, são igualmente eficientes nas três variedades. Houve correlação entre o peso seco das plantas e o nitrogênio fixado, porém a diferenciação das estirpes pelo peso seco das plantas perdeu em sensibilidade. A nodulação não foi critério satisfatório para separar estirpes eficientes. Para o conjunto de estirpes eficientes em pelo menos uma das variedades estudadas, verificou-se que viçoja apresentou menor especificidade, estabelecendo simbiose eficaz com nove das estirpes testadas.

Influence of soybean inoculation and nitrogen levels on populations and serogroups of Rhizobium japonicum in Ontario

1979 ◽  
Vol 25 (6) ◽  
pp. 739-745 ◽  
Author(s):  
Ernest Semu ◽  
D. J. Hume ◽  
C. T. Corke
Keyword(s):  
Glycine Max ◽  
Rhizobium Japonicum ◽  
Most Probable Number ◽  
Nitrogen Application ◽  
Fertilizer N ◽  
Probable Number ◽  
Fertilizer Nitrogen ◽  
Nitrogen Levels ◽  
Glycine Max L

Strains and numbers of Rhizobium japonicum in the soil can influence soybean (Glycine max (L.) Merrill) yields but R. japonicum serogroups and numbers in Ontario soils have not been studied. In 1976 and 1977, the influences of inoculation and fertilizer nitrogen (N) levels on populations, measured by most probable number (MPN) techniques, and serogroups of R. japonicum were studied at three Ontario sites. The locations, near Ridgetown, Elora, and Woodstock, represented areas where soybeans had been grown many times previously, only a few times or not at all, respectively. Treatments were (a) un inoculated + O N; (b–e) inoculated +0, 50, 100 or 200 kg N/ha.At Ridgetown, MPN's, expressed on log10 bases, ranged from 5.17/g soil in 1977 to 7.02 in 1976. At Elora, comparable values were from 3.66 to 6.43. Inoculation did not increase MPN's at either site. At Woodstock, inoculation increased MPN's from 0 to about 2.47 in both years. Nitrogen application did not change the MPN values at any site in either year.Serogroup 110 predominated in the nodules at Ridgetown in both years. At Elora, serogroup 123 was found most frequently in nodules. Serogroups 311b 138 in 1976 and 122 and 125 in 1977 were found most frequently after inoculation at Woodstock. Additions of fertilizer N had only slight effects on the distribution of serogroups in the nodules.

Penicillin sensitivity of nodulation in soybean roots

1984 ◽  
Vol 30 (1) ◽  
pp. 77-80 ◽  
Author(s):  
S. T. Takats
Keyword(s):  
Glycine Max ◽  
Frequency Distribution ◽  
Rhizobium Japonicum ◽  
Bacterial Viability ◽  
Peptidoglycan Synthesis ◽  
One Stage ◽  
Primary Roots ◽  
Glycine Max L ◽  
Soybean Roots ◽  
And Shifts

Penicillin was administered to the primary roots of soybean (Glycine max. L. Merr) at the time of inoculation with Rhizobium japonicum and at various times after inoculation. Penicillin reduces nodule yield and shifts the frequency distribution of nodules along the primary roots. When administered at the time of inoculation, the earliest nodules do not form; when administered at later times, from 10 to 72 h after inoculation, the later nodules are reduced in number. These effects, when related to parallel effects of penicillin on bacterial viability, peptidoglycan synthesis, and binding to the roots suggest that penicillin interferes with more than one stage of nodule induction.

EFFECT OF DIFFERENT INOCULANT ADHESIVE AGENTS ON RHIZOBIAL SURVIVAL, NODULATION, AND NITROGENASE (ACETYLENE-REDUCING) ACTIVITY OF SOYBEANS (Glycine max (L.) Merrill)

1984 ◽  
Vol 64 (4) ◽  
pp. 631-636 ◽  
Author(s):  
M. S. ELEGBA ◽  
R. J. RENNIE
Keyword(s):  
Glycine Max ◽  
Key Words ◽  
Carboxymethyl Cellulose ◽  
Gum Arabic ◽  
Rhizobium Japonicum ◽  
Market Place ◽  
Canadian Soils ◽  
Glycine Max L ◽  
Reducing Activity ◽  
Acetylene Reducing Activity

Inoculation of soybeans is essential to the establishment of a successful N2-fixing symbiosis in western Canadian soils that lack indigenous soil Rhizobium japonicum. A comparison was thus made between commercial and non-commercial adhesive agents used to optimize inoculation of King Grain line X005 soybeans (Glycine max (L.) Merrill) with seed-applied powdered peat-based inoculant. Gum arabic (40%, wt/vol) and carboxymethyl cellulose (4% wt/vol), available only from chemical supply houses, were excellent adhesive agents, binding over 800 mg of the inoculant per seed and protecting bacteria from desiccation. Wallpaper glue (10%, wt/vol), readily available in the market place and in common use by some farmers, bound 894 mg of inoculant per seed. The two most readily available commercial adhesive agents, Nutrigum and Nitracoat, bound similar amounts of inoculant. The use of water as an adhesive agent did not nourish the bacteria or protect them from desiccation and can not be recommended. All 12 adhesive agents tested resulted in over 105 viable rhizobia per seed, satisfying inoculation requirements under the Fertilizers Act. However, the three best adhesive agents bound over 800 mg of inoculant to the seed resulting in over 106 viable rhizobia per seed and more than 100 nodules per plant. Key words: Rhizobium japonicum, N2 fixation

Sign in / Sign up

Export Citation Format

Share Document