Influence of soybean inoculation and nitrogen levels on populations and serogroups of Rhizobium japonicum in Ontario

1979 ◽  
Vol 25 (6) ◽  
pp. 739-745 ◽  
Author(s):  
Ernest Semu ◽  
D. J. Hume ◽  
C. T. Corke

Strains and numbers of Rhizobium japonicum in the soil can influence soybean (Glycine max (L.) Merrill) yields but R. japonicum serogroups and numbers in Ontario soils have not been studied. In 1976 and 1977, the influences of inoculation and fertilizer nitrogen (N) levels on populations, measured by most probable number (MPN) techniques, and serogroups of R. japonicum were studied at three Ontario sites. The locations, near Ridgetown, Elora, and Woodstock, represented areas where soybeans had been grown many times previously, only a few times or not at all, respectively. Treatments were (a) un inoculated + O N; (b–e) inoculated +0, 50, 100 or 200 kg N/ha.At Ridgetown, MPN's, expressed on log10 bases, ranged from 5.17/g soil in 1977 to 7.02 in 1976. At Elora, comparable values were from 3.66 to 6.43. Inoculation did not increase MPN's at either site. At Woodstock, inoculation increased MPN's from 0 to about 2.47 in both years. Nitrogen application did not change the MPN values at any site in either year.Serogroup 110 predominated in the nodules at Ridgetown in both years. At Elora, serogroup 123 was found most frequently in nodules. Serogroups 311b 138 in 1976 and 122 and 125 in 1977 were found most frequently after inoculation at Woodstock. Additions of fertilizer N had only slight effects on the distribution of serogroups in the nodules.

1979 ◽  
Vol 59 (4) ◽  
pp. 1129-1137 ◽  
Author(s):  
ERNEST SEMU ◽  
D. J. HUME

Soybeans (Glycine max (L.) Merrill) often do not give yield responses to added fertilizer nitrogen (N) because high soil N levels inhibit fixation of atmospheric N2. Yield responses to N fertilizer applied at planting usually indicate that N2 fixation is less than optimal. The effects of inoculation with Rhizobium japonicum, and fertilizer N levels, on soybean N2(C2H2) fixation and seed yields in Ontario were investigated in ’ 1976 and 1977. Three locations were used each year, representing areas where soybeans had been grown for many years (Ridgetown), for only a few years (Elora), or not at all (Woodstock). Treatments were (a) Uninoculated + 0 N, (b–e) Inoculated + 0, 50, 100 or 200 kg N/ha. Results indicated that inoculation increased seed yields only when soybeans were introduced into new areas. Fertilizer N applications at planting time did not increase yields in areas where soybeans had been grown several times previously, indicating that N2 fixation could support maximum yields. Nodule number and mass, and N2(C2H2) fixation rates were all decreased by fertilizer N. An increase in nodule efficiency, later in the season, in high N treatments was most marked at Ridgetown.


1980 ◽  
Vol 66 (6) ◽  
pp. 1027-1031 ◽  
Author(s):  
T. V. Bhuvaneswari ◽  
B. Gillian Turgeon ◽  
Wolfgang D. Bauer

1997 ◽  
Vol 77 (4) ◽  
pp. 543-551 ◽  
Author(s):  
F. S. Rembon ◽  
A. F. MacKenzie

Soybean (Glycine max L. Merill) can produce high-N residues that may benefit subsequent corn (Zea mays L.) production, but the degree of benefit is often unpredictable and may be related to tillage methods. This study investigated the effects of conventional-tillage (CT) and no-tillage (NT) on fertilizer replacement values for corn in a corn-soybean rotation. Field experiments were conducted for two growing seasons on two soils, a Ste. Rosalie clay (Humic Gleysol), and an Ormstown silty clay (Humic Gleysol). Continuous corn, corn following soybean, soybean following corn, continuous soybean, and three levels of fertilizer N (0, 90, 180 and 0, 20, and 40 kg N ha−1 for corn and soybean, respectively) were compared. Tillage did not effect yield or N uptake consistently. Corn grain yields and N uptake were greater following soybean than following corn. Soybean provided N fertilizer credits ranging from 40 to 150 kg N ha−1, which was greater than the residual NO3 in the soil prior to planting. Credits were greater in the year with higher corn yields and lower previous winter precipitation resulting in greater NO3 carryover. Tillage effects on N credits from soybean differed between the sites. Consequently, N contributions of soybean to corn could not be related to tillage method or soil type. Key words:Zea mays L., Glycine max L. Merill, rotations, grain yield, N uptake, tillage, fertilizer N


1983 ◽  
Vol 63 (3) ◽  
pp. 641-647 ◽  
Author(s):  
S. DUBETZ ◽  
D. J. MAJOR ◽  
R. J. RENNIE

Four experiments were conducted at two locations in southern Alberta, where no indigenous Rhizobium japonicum existed, to determine the effects of seeding dates, fertilizers, irrigation, and method of inoculant placement on the yield and quality of several early-maturing soybean (Glycine max (L.) Merrill) cultivars. Highest yields were obtained when soybeans were seeded during the first half of May. Irrigation requirements varied between years but yields were similar whether soil moisture was depleted to the 6 × 104-Pa or the 3 × 104-Pa level before irrigating to field capacity. Soybean yields increased substantially from N fertilizer when the inoculant was applied to the seed without a sticker. When granular inoculant was banded with the seed there was no response to N, and the yields of inoculated plants were higher than those of plants that were uninoculated and received up to 160 kg N/ha. When properly inoculated, early-maturing cultivars such as King Grain strain X005 and Maple Presto were capable of maturing and producing seed of acceptable yield and quality in southern Alberta.Key words: Cultivars, fertilizer, irrigation, inoculant, Glycine max (L.) Merrill


1985 ◽  
Vol 63 (11) ◽  
pp. 1951-1956 ◽  
Author(s):  
W. H. Fuchsman ◽  
R. G. Palmer

The leghemoglobins from a genetically diverse selection of 69 cultivated soybean (Glycine max (L.) Merr.) cultivars and plant introductions and 18 wild soybean (Glycine soja Sieb. & Zucc.) plant introductions all consist of the same set of major leghemoglobins (a, c1, c2, c3), as determined by analytical isoelectric focusing. The conservation of both leghemoglobin heterogeneity and also all four major leghemoglobin structures provides strong circumstantial evidence that leghemoglobin heterogeneity is functional. Glycine max and G. soja produced the same leghemoglobins in the presence of Bradyrhizobium japonicum (Kirchner) Jordan and in the presence of fast-growing Rhizobium japonicum.


Bragantia ◽  
1976 ◽  
Vol 35 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Eli Sidney Lopes ◽  
Antônio Roberto Giardini ◽  
Romeu A. S. Kiihl ◽  
Toshio Igue

Um experimento foi conduzido em casa de vegetação, em vasos de Leonard com solução nutritiva sem nitrogênio, em condições assépticas, com os objetivos de pré-selecionar treze estirpes de Rhizobium japonicum e verificar o grau e especificidade das variedades santa-rosa, viçoja e IAC-2 (Glycine max (L.) Merril.). Pela observação do nitrogênio total fixado aos 58 dias, verificou-se que as estirpes SMS-64 (= 513 Re, RS), SMS-65 (= 519 Re, RS), SMS-68 (= 532c, RS = PR-G3, IBPT, PR) SMS-309 e SMS-310, são igualmente eficientes nas três variedades. Houve correlação entre o peso seco das plantas e o nitrogênio fixado, porém a diferenciação das estirpes pelo peso seco das plantas perdeu em sensibilidade. A nodulação não foi critério satisfatório para separar estirpes eficientes. Para o conjunto de estirpes eficientes em pelo menos uma das variedades estudadas, verificou-se que viçoja apresentou menor especificidade, estabelecendo simbiose eficaz com nove das estirpes testadas.


2019 ◽  
Vol 5 (1) ◽  
pp. 6
Author(s):  
Rika Agustina ◽  
Hamidah Hamidah ◽  
Akhmad Sopian

Giving Superfarm Rhizobium and N fertilizer on the Growth and Production of Soybean (Glycine max L. Merill.). The aim of research to determine the effect of Superfarm Rhizobium and N fertilizer on the growth and yield of soybean (Glycine max (L. Merill). The study was conducted over four months, from March to July 2015. The study in Dusun Desa Sumber Jaya Manunggal Jaya Rt. 09 District of Tenggarong Seberang Regency. this study used a draft split plot with factorial 3 x 4 consisting of three replications. the first factor is Superfarm Rhizobium which comprise three levels: ie s0 (without treatment), s1 (2 g / plant), s2 (4 g / plant). The second factor is the dose of N fertilizer consists of four levels ie N0 (without fertilizer) n1 (2.5G / plant) n2 (5g / plant) and n3 (7,5g / plant) . the results showed that treatment of Rhizobium Superfarm Award (S) had no significant effect on all parameters. While the provision of fertilizer N (N) significantly affected plant height parameter parameter 2, 4 and 6 weeks after planting. and at a dose of 7.5 g significantly the parameters of crop seed weight, seed weight per plot, the production results (Ton ha-1). The highest production was achieved by treatment s0n3 483.33 g / plot, equivalent to 2.69 Toh ha-1.


Author(s):  
Ladislav Ducsay ◽  
Ladislav Varga ◽  
Pavel Ryant

Pot trial was aimed to investigate an effect of fertilizer nitrogen on the content of vitamin C and offtake of certain macroelements by Brassica pekinensis. The experiment was realized in the years 2000 and 2001 in pots containing 10 kg of loamy brownsoil. Optimalization of NPK rates contributed to the increase of N, P, K, Mg and S offtake by the yield of final product in comparison with unfertilized control. The most marked increase offtake of nitrogen (by 14,6%) was obtained when fertilizer N was applied in the diveded form (80% N prior to seeding + 20% N in initial phase of heads formation, nitrogen applied as DAM 390). Positive effect of full NPK nutrition on the vitamin C content was determined. The highest content of vitamin C (629.0 mg.kg-1 of fresh mass) was determined in treatment where nitrogen was applied in the divided rate.


1984 ◽  
Vol 30 (1) ◽  
pp. 77-80 ◽  
Author(s):  
S. T. Takats

Penicillin was administered to the primary roots of soybean (Glycine max. L. Merr) at the time of inoculation with Rhizobium japonicum and at various times after inoculation. Penicillin reduces nodule yield and shifts the frequency distribution of nodules along the primary roots. When administered at the time of inoculation, the earliest nodules do not form; when administered at later times, from 10 to 72 h after inoculation, the later nodules are reduced in number. These effects, when related to parallel effects of penicillin on bacterial viability, peptidoglycan synthesis, and binding to the roots suggest that penicillin interferes with more than one stage of nodule induction.


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