scholarly journals A stable, high refractive index, switching buffer for super-resolution imaging

2018 ◽  
Author(s):  
Tobias M.P. Hartwich ◽  
Kenny Kwok Hin Chung ◽  
Lena Schroeder ◽  
Joerg Bewersdorf ◽  
Christian Soeller ◽  
...  
Keyword(s):  
Refractive Index ◽  
Super Resolution ◽  
High Refractive Index ◽  
Dye Molecules ◽  
Switching Speed ◽  
Dark State ◽  
Oxygen Scavenging ◽  
Index Matching ◽  
Improved Stability ◽  
Resolution Imaging

AbstractdSTORM super-resolution imaging relies on switching buffers to enable dye molecules to enter and exit a metastable dark state. Current buffers have a very limited shelf life of approximately 1 day and poorly match sample refractive index, impacting negatively on measurement reproducibility and image fidelity. We present a buffer based on chemical, rather than enzymatic, oxygen scavenging which exhibits dramatically improved stability, switching speed, contrast, and index matching.

scholarly journals Coated High-Refractive-Index Barium Titanate Glass Microspheres for Optically Trapped Microsphere Super-Resolution Microscopy: A Simulation Study

Photonics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 84
Author(s):  
Xi Liu ◽  
Song Hu ◽  
Yan Tang
Keyword(s):  
Refractive Index ◽  
Barium Titanate ◽  
Optical Trapping ◽  
Super Resolution ◽  
High Refractive Index ◽  
Trapping Efficiency ◽  
Single Beam ◽  
Imaging Performance ◽  
Super Resolution Microscopy ◽  
Optically Trapped

As water is normally used as the immersion medium in optically trapped microsphere microscopy, the high-refractive-index barium titanate glass (BTG) microsphere shows a better imaging performance than the low-index polystyrene (PS) or melamine formaldehyde (MF) microsphere, but it is difficult to be trapped by single-beam optical trapping due to its overly high refractive index. In this study, coated BTG microspheres with a PS coating have been computationally explored for the combination of optical trapping with microsphere-assisted microscopy. The PS coating thickness affects both the optical trapping efficiency and photonic nanojet (PNJ) property of the coated BTG sphere. Compared to the uncoated BTG sphere, the coated BTG sphere with a proper PS coating thickness has a highly improved trapping efficiency which enables single-beam optical trapping, and a better PNJ with a higher optical intensity Imax and a narrower full width at half maximum (FWHM) corresponding to better imaging performance. These coated BTG spheres also have an advantage in trapping efficiency and imaging performance over conventional PS and MF spheres. The coated BTG microsphere is highly desirable for optically trapped microsphere super-resolution microscopy and potentially beneficial to other research areas, such as nanoparticle detection.

scholarly journals Rapid single-wavelength lightsheet localization microscopy for clarified tissue

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Li-An Chu ◽  
Chieh-Han Lu ◽  
Shun-Min Yang ◽  
Yen-Ting Liu ◽  
Kuan-Lin Feng ◽  
...  
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Biological Tissues ◽  
Adult Brain ◽  
Imaging Data ◽  
Transporter Protein ◽  
Index Matching ◽  
Refractive Index Matching ◽  
Nanoscale Imaging ◽  
Resolution Imaging

Abstract Optical super-resolution microscopy allows nanoscale imaging of protein molecules in intact biological tissues. However, it is still challenging to perform large volume super-resolution imaging for entire animal organs. Here we develop a single-wavelength Bessel lightsheet method, optimized for refractive-index matching with clarified specimens to overcome the aberrations encountered in imaging thick tissues. Using spontaneous blinking fluorophores to label proteins of interest, we resolve the morphology of most, if not all, dopaminergic neurons in the whole adult brain (3.64 × 107 µm3) of Drosophila melanogaster at the nanometer scale with high imaging speed (436 µm3 per second) for localization. Quantitative single-molecule localization reveals the subcellular distribution of a monoamine transporter protein in the axons of a single, identified serotonergic Dorsal Paired Medial (DPM) neuron. Large datasets are obtained from imaging one brain per day to provide a robust statistical analysis of these imaging data.

scholarly journals Terahertz Computed Tomography of High-Refractive-Index Objects Based on Refractive Index Matching

2018 ◽  
Vol 10 (6) ◽  
pp. 1-13
Author(s):  
Linyu Chen ◽  
Yuye Wang ◽  
Degang Xu ◽  
Yuchen Ren ◽  
Yixin He ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Refractive Index ◽  
High Refractive Index ◽  
Index Matching ◽  
Refractive Index Matching

scholarly journals Synthesis and super-resolution imaging performance of a refractive-index-controllable microsphere superlens

2015 ◽  
Vol 3 (41) ◽  
pp. 10907-10915 ◽  
Author(s):  
Haie Zhu ◽  
Bing Yan ◽  
Shuxue Zhou ◽  
Zengbo Wang ◽  
Limin Wu
Keyword(s):  
Refractive Index ◽  
Super Resolution ◽  
Resolution Imaging ◽  
Imaging Performance

Enhancement of Afterglow Luminescence of Long-Lasting Phosphor-Glass Composite by Using Refractive Index Matched Glass

2016 ◽  
Vol 702 ◽  
pp. 113-117 ◽  
Author(s):  
Masaru Yamashita ◽  
Toshinori Imamura ◽  
Sachiko Matsumoto ◽  
Masaki Murakami ◽  
Toshiaki Hongo ◽  
...  
Keyword(s):  
Refractive Index ◽  
Glass Composition ◽  
High Refractive Index ◽  
Dark Field ◽  
Glass Composite ◽  
Thick Sample ◽  
Index Matching ◽  
Digital Microscope ◽  
Refractive Index Matching ◽  
Lower Refractive Index

Composites of a long-lasting phosphor, SrAl2O4:Eu,Dy, and glass were prepared by sintering the phosphor and glass powder. To enhance the afterglow luminescence, a borosilicate glass composition was chosen so that the refractive index of the glass matched that of the phosphor. Additional components with a high refractive index, such as La2O3 and Nb2O5, were added to the glass to increase the overall refractive index. As they tend to induce crystallization during sintering, small amounts of at least three types of such components were added to the glass to prevent crystallization. The surface of the composite was observed by a digital microscope with dark-field lighting. The phosphor particles became almost transparent because of the refractive-index matching, although bubbles were observed inside the phosphor particles. The afterglow luminance was, however, almost the same and the transmittance of the composite was not high because of many voids when compared to as that of the sample using the glass with a lower refractive index. The sample prepared under vacuum showed coloration and similar afterglow luminance even though the number of voids inside the composite decreased. To suppress the coloration, the amount of tin in the glass was increased, after which higher transparency and afterglow luminance were obtained. A 4-mm-thick sample showed a luminance of 118 mcd∙m-2 60 min after irradiation by a D65 lamp with 200 lx for 20 min.

A polymer index-matched to water enables diverse applications in fluorescence microscopy

Lab on a Chip ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 1549-1562
Author(s):  
Xiaofei Han ◽  
Yijun Su ◽  
Hamilton White ◽  
Kate M. O'Neill ◽  
Nicole Y. Morgan ◽  
...  
Keyword(s):  
Refractive Index ◽  
Fluorescence Microscopy ◽  
Biological Samples ◽  
Super Resolution ◽  
Resolution Imaging

Diffraction-limited and super-resolution imaging of biological samples using refractive-index matched polymers in microdevices.

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