A polymer index-matched to water enables diverse applications in fluorescence microscopy

Lab on a Chip ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 1549-1562
Author(s):  
Xiaofei Han ◽  
Yijun Su ◽  
Hamilton White ◽  
Kate M. O'Neill ◽  
Nicole Y. Morgan ◽  
...  
Keyword(s):  
Refractive Index ◽  
Fluorescence Microscopy ◽  
Biological Samples ◽  
Super Resolution ◽  
Resolution Imaging

Diffraction-limited and super-resolution imaging of biological samples using refractive-index matched polymers in microdevices.

scholarly journals Accelerated FRET-PAINT Microscopy

2018 ◽  
Author(s):  
Jongjin Lee ◽  
Sangjun Park ◽  
Sungchul Hohng
Keyword(s):  
Fluorescence Microscopy ◽  
Super Resolution ◽  
Dissociation Rate ◽  
Dna Probes ◽  
Speed Limit ◽  
High Quality ◽  
Resolution Imaging ◽  
Bleed Through ◽  
Imaging Speed

Recent development of FRET-PAINT microscopy significantly improved the imaging speed of DNA-PAINT, the previously reported super-resolution fluorescence microscopy with no photobleaching problem. Here we try to achieve the ultimate speed limit of FRET-PAINT by optimizing the camera speed, dissociation rate of DNA probes, and bleed-through of the donor signal to the acceptor channel, and further increase the imaging speed of FRET-PAINT by 8-fold. Super-resolution imaging of COS-7 microtubules shows that high-quality 40-nm resolution images can be obtained in just tens of seconds.

scholarly journals Three-Dimensional Super-resolution Imaging of Thick Biological Samples

2009 ◽  
Vol 15 (S2) ◽  
pp. 36-37
Author(s):  
A Vaziri ◽  
J Tang ◽  
H Shroff ◽  
C Shank
Keyword(s):  
Biological Samples ◽  
Three Dimensional ◽  
Super Resolution ◽  
Resolution Imaging

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009

scholarly journals Label-free super-resolution imaging below 90-nm using photon-reassignment

2021 ◽  
Vol 1 ◽  
pp. 3
Author(s):  
Alberto Aguilar ◽  
Adeline Boyreau ◽  
Pierre Bon
Keyword(s):  
Biological Samples ◽  
Optical Resolution ◽  
Super Resolution ◽  
Cost Effective ◽  
Label Free ◽  
Cost Effective Approach ◽  
Imaging Mode ◽  
Resolution Imaging ◽  
Fluorescent Tags ◽  
Super Resolution Microscopy

Background: Achieving resolutions below 100 nm is key for many fields, including biology and nanomaterial characterization. Although nearfield and electron microscopy are the gold standards for studying the nanoscale, optical microscopy has seen its resolution drastically improve in the last decades. So-called super-resolution microscopy is generally based on fluorescence photophysics and requires modification of the sample at least by adding fluorescent tags, an inevitably invasive step. Therefore, it remains very challenging and rewarding to achieve optical resolutions beyond the diffraction limit in label-free samples. Methods: Here, we present a breakthrough to unlock label-free 3D super-resolution imaging of any object including living biological samples. It is based on optical photon-reassignment in confocal reflectance imaging mode. Results: We demonstrate that we surpass the resolution of all fluorescence-based confocal systems by a factor ~1.5. We have obtained images with a 3D (x,y,z) optical resolution of (86x86x248) nm3 using a visible wavelength (445 nm) and a regular microscope objective (NA=1.3). The results are presented on nanoparticles as well as on (living) biological samples. Conclusions: This cost-effective approach double the resolution of reflectance confocal microscope with minimal modifications. It is therefore compatible with any microscope and sample, works in real-time, and does not require any signal processing.

scholarly journals Multilayer Three-dimensional Super-resolution Imaging of Thick Biological Samples

2009 ◽  
Vol 96 (3) ◽  
pp. 17a
Author(s):  
Alipasha Vaziri ◽  
Jianyong Tang ◽  
Hari Shroff ◽  
Charles Shank
Keyword(s):  
Biological Samples ◽  
Three Dimensional ◽  
Super Resolution ◽  
Resolution Imaging

scholarly journals Recent Progress in Light Sheet Microscopy for Biological Applications

2018 ◽  
Vol 72 (8) ◽  
pp. 1137-1169 ◽  
Author(s):  
Krishnendu Chatterjee ◽  
Feby Wijaya Pratiwi ◽  
Frances Camille M. Wu ◽  
Peilin Chen ◽  
Bi-Chang Chen
Keyword(s):  
Developmental Biology ◽  
Fluorescence Microscopy ◽  
Single Cell ◽  
Single Molecule ◽  
Super Resolution ◽  
Light Sheet ◽  
High Signal ◽  
Spatiotemporal Resolution ◽  
Light Sheet Microscopy ◽  
Resolution Imaging

The introduction of light sheet fluorescence microscopy (LSFM) has overcome the challenges in conventional optical microscopy. Among the recent breakthroughs in fluorescence microscopy, LSFM had been proven to provide a high three-dimensional spatial resolution, high signal-to-noise ratio, fast imaging acquisition rate, and minuscule levels of phototoxic and photodamage effects. The aforementioned auspicious properties are crucial in the biomedical and clinical research fields, covering a broad range of applications: from the super-resolution imaging of intracellular dynamics in a single cell to the high spatiotemporal resolution imaging of developmental dynamics in an entirely large organism. In this review, we provided a systematic outline of the historical development of LSFM, detailed discussion on the variants and improvements of LSFM, and delineation on the most recent technological advancements of LSFM and its potential applications in single molecule/particle detection, single-molecule super-resolution imaging, imaging intracellular dynamics of a single cell, multicellular imaging: cell–cell and cell–matrix interactions, plant developmental biology, and brain imaging and developmental biology.

scholarly journals Synthesis and super-resolution imaging performance of a refractive-index-controllable microsphere superlens

2015 ◽  
Vol 3 (41) ◽  
pp. 10907-10915 ◽  
Author(s):  
Haie Zhu ◽  
Bing Yan ◽  
Shuxue Zhou ◽  
Zengbo Wang ◽  
Limin Wu
Keyword(s):  
Refractive Index ◽  
Super Resolution ◽  
Resolution Imaging ◽  
Imaging Performance

scholarly journals Development of targeted STORM for super resolution imaging of biological samples using digital micro-mirror device

2017 ◽  
Vol 404 ◽  
pp. 18-22 ◽  
Author(s):  
Liyana Valiya Peedikakkal ◽  
Victoria Steventon ◽  
Andrew Furley ◽  
Ashley J. Cadby
Keyword(s):  
Biological Samples ◽  
Super Resolution ◽  
Resolution Imaging
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