Synthesis of High Refractive Index and Shape Controllable Colloidal Polymer Microspheres for Super-Resolution Imaging

2017 ◽  
Vol 50 (2) ◽  
pp. 660-665 ◽  
Author(s):  
Haie Zhu ◽  
Min Chen ◽  
Shuxue Zhou ◽  
Limin Wu
Keyword(s):  
Refractive Index ◽  
Super Resolution ◽  
High Refractive Index ◽  
Polymer Microspheres ◽  
Resolution Imaging

scholarly journals A stable, high refractive index, switching buffer for super-resolution imaging

2018 ◽  
Author(s):  
Tobias M.P. Hartwich ◽  
Kenny Kwok Hin Chung ◽  
Lena Schroeder ◽  
Joerg Bewersdorf ◽  
Christian Soeller ◽  
...  
Keyword(s):  
Refractive Index ◽  
Super Resolution ◽  
High Refractive Index ◽  
Dye Molecules ◽  
Switching Speed ◽  
Dark State ◽  
Oxygen Scavenging ◽  
Index Matching ◽  
Improved Stability ◽  
Resolution Imaging

AbstractdSTORM super-resolution imaging relies on switching buffers to enable dye molecules to enter and exit a metastable dark state. Current buffers have a very limited shelf life of approximately 1 day and poorly match sample refractive index, impacting negatively on measurement reproducibility and image fidelity. We present a buffer based on chemical, rather than enzymatic, oxygen scavenging which exhibits dramatically improved stability, switching speed, contrast, and index matching.

scholarly journals Coated High-Refractive-Index Barium Titanate Glass Microspheres for Optically Trapped Microsphere Super-Resolution Microscopy: A Simulation Study

Photonics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 84
Author(s):  
Xi Liu ◽  
Song Hu ◽  
Yan Tang
Keyword(s):  
Refractive Index ◽  
Barium Titanate ◽  
Optical Trapping ◽  
Super Resolution ◽  
High Refractive Index ◽  
Trapping Efficiency ◽  
Single Beam ◽  
Imaging Performance ◽  
Super Resolution Microscopy ◽  
Optically Trapped

As water is normally used as the immersion medium in optically trapped microsphere microscopy, the high-refractive-index barium titanate glass (BTG) microsphere shows a better imaging performance than the low-index polystyrene (PS) or melamine formaldehyde (MF) microsphere, but it is difficult to be trapped by single-beam optical trapping due to its overly high refractive index. In this study, coated BTG microspheres with a PS coating have been computationally explored for the combination of optical trapping with microsphere-assisted microscopy. The PS coating thickness affects both the optical trapping efficiency and photonic nanojet (PNJ) property of the coated BTG sphere. Compared to the uncoated BTG sphere, the coated BTG sphere with a proper PS coating thickness has a highly improved trapping efficiency which enables single-beam optical trapping, and a better PNJ with a higher optical intensity Imax and a narrower full width at half maximum (FWHM) corresponding to better imaging performance. These coated BTG spheres also have an advantage in trapping efficiency and imaging performance over conventional PS and MF spheres. The coated BTG microsphere is highly desirable for optically trapped microsphere super-resolution microscopy and potentially beneficial to other research areas, such as nanoparticle detection.

scholarly journals Synthesis and super-resolution imaging performance of a refractive-index-controllable microsphere superlens

2015 ◽  
Vol 3 (41) ◽  
pp. 10907-10915 ◽  
Author(s):  
Haie Zhu ◽  
Bing Yan ◽  
Shuxue Zhou ◽  
Zengbo Wang ◽  
Limin Wu
Keyword(s):  
Refractive Index ◽  
Super Resolution ◽  
Resolution Imaging ◽  
Imaging Performance

A polymer index-matched to water enables diverse applications in fluorescence microscopy

Lab on a Chip ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 1549-1562
Author(s):  
Xiaofei Han ◽  
Yijun Su ◽  
Hamilton White ◽  
Kate M. O'Neill ◽  
Nicole Y. Morgan ◽  
...  
Keyword(s):  
Refractive Index ◽  
Fluorescence Microscopy ◽  
Biological Samples ◽  
Super Resolution ◽  
Resolution Imaging

Diffraction-limited and super-resolution imaging of biological samples using refractive-index matched polymers in microdevices.

Accelerated Discovery of High-Refractive-Index Polyimides via First-Principles Molecular Modeling, Virtual High-Throughput Screening, and Data Mining

2019 ◽  
Author(s):  
Mohammad Atif Faiz Afzal ◽  
Mojtaba Haghighatlari ◽  
Sai Prasad Ganesh ◽  
Chong Cheng ◽  
Johannes Hachmann
Keyword(s):  
Data Mining ◽  
Refractive Index ◽  
High Throughput ◽  
First Principles ◽  
High Throughput Screening ◽  
Large Scale ◽  
Computational Study ◽  
High Refractive Index ◽  
Structural Features ◽  
Learning Program

<div>We present a high-throughput computational study to identify novel polyimides (PIs) with exceptional refractive index (RI) values for use as optic or optoelectronic materials. Our study utilizes an RI prediction protocol based on a combination of first-principles and data modeling developed in previous work, which we employ on a large-scale PI candidate library generated with the ChemLG code. We deploy the virtual screening software ChemHTPS to automate the assessment of this extensive pool of PI structures in order to determine the performance potential of each candidate. This rapid and efficient approach yields a number of highly promising leads compounds. Using the data mining and machine learning program package ChemML, we analyze the top candidates with respect to prevalent structural features and feature combinations that distinguish them from less promising ones. In particular, we explore the utility of various strategies that introduce highly polarizable moieties into the PI backbone to increase its RI yield. The derived insights provide a foundation for rational and targeted design that goes beyond traditional trial-and-error searches.</div>

Sign in / Sign up

Export Citation Format

Share Document