Rapid super-resolution imaging of sub-surface nanostructures beyond diffraction limit by high refractive index microsphere optical nanoscopy

2015 ◽  
Vol 334 ◽  
pp. 253-257 ◽  
Author(s):  
Seoungjun Lee ◽  
Lin Li
Keyword(s):  
Refractive Index ◽  
Super Resolution ◽  
High Refractive Index ◽  
Diffraction Limit ◽  
Surface Nanostructures ◽  
Resolution Imaging

scholarly journals A stable, high refractive index, switching buffer for super-resolution imaging

2018 ◽  
Author(s):  
Tobias M.P. Hartwich ◽  
Kenny Kwok Hin Chung ◽  
Lena Schroeder ◽  
Joerg Bewersdorf ◽  
Christian Soeller ◽  
...  
Keyword(s):  
Refractive Index ◽  
Super Resolution ◽  
High Refractive Index ◽  
Dye Molecules ◽  
Switching Speed ◽  
Dark State ◽  
Oxygen Scavenging ◽  
Index Matching ◽  
Improved Stability ◽  
Resolution Imaging

AbstractdSTORM super-resolution imaging relies on switching buffers to enable dye molecules to enter and exit a metastable dark state. Current buffers have a very limited shelf life of approximately 1 day and poorly match sample refractive index, impacting negatively on measurement reproducibility and image fidelity. We present a buffer based on chemical, rather than enzymatic, oxygen scavenging which exhibits dramatically improved stability, switching speed, contrast, and index matching.

scholarly journals Coated High-Refractive-Index Barium Titanate Glass Microspheres for Optically Trapped Microsphere Super-Resolution Microscopy: A Simulation Study

Photonics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 84
Author(s):  
Xi Liu ◽  
Song Hu ◽  
Yan Tang
Keyword(s):  
Refractive Index ◽  
Barium Titanate ◽  
Optical Trapping ◽  
Super Resolution ◽  
High Refractive Index ◽  
Trapping Efficiency ◽  
Single Beam ◽  
Imaging Performance ◽  
Super Resolution Microscopy ◽  
Optically Trapped

As water is normally used as the immersion medium in optically trapped microsphere microscopy, the high-refractive-index barium titanate glass (BTG) microsphere shows a better imaging performance than the low-index polystyrene (PS) or melamine formaldehyde (MF) microsphere, but it is difficult to be trapped by single-beam optical trapping due to its overly high refractive index. In this study, coated BTG microspheres with a PS coating have been computationally explored for the combination of optical trapping with microsphere-assisted microscopy. The PS coating thickness affects both the optical trapping efficiency and photonic nanojet (PNJ) property of the coated BTG sphere. Compared to the uncoated BTG sphere, the coated BTG sphere with a proper PS coating thickness has a highly improved trapping efficiency which enables single-beam optical trapping, and a better PNJ with a higher optical intensity Imax and a narrower full width at half maximum (FWHM) corresponding to better imaging performance. These coated BTG spheres also have an advantage in trapping efficiency and imaging performance over conventional PS and MF spheres. The coated BTG microsphere is highly desirable for optically trapped microsphere super-resolution microscopy and potentially beneficial to other research areas, such as nanoparticle detection.

Super-resolution imaging of SERS hot spots

2014 ◽  
Vol 43 (11) ◽  
pp. 3854-3864 ◽  
Author(s):  
Katherine A. Willets
Keyword(s):  
Hot Spots ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Resolution Imaging

Super-resolution imaging defeats the diffraction-limit of light, allowing the spatial origin and intensity of SERS signals to be determined with <5 nm resolution.

scholarly journals Focus on Super-Resolution Imaging with Direct Stochastic Optical Reconstruction Microscopy (dSTORM)

2014 ◽  
Vol 67 (2) ◽  
pp. 179 ◽  
Author(s):  
Donna R. Whelan ◽  
Thorge Holm ◽  
Markus Sauer ◽  
Toby D. M. Bell
Keyword(s):  
Cell Biology ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Stochastic Optical Reconstruction Microscopy ◽  
Resolution Imaging ◽  
Optical Reconstruction ◽  
Set Up ◽  
Microscopy Techniques ◽  
Super Resolution Microscopy ◽  
Microscopic Techniques

The last decade has seen the development of several microscopic techniques capable of achieving spatial resolutions that are well below the diffraction limit of light. These techniques, collectively referred to as ‘super-resolution’ microscopy, are now finding wide use, particularly in cell biology, routinely generating fluorescence images with resolutions in the order of tens of nanometres. In this highlight, we focus on direct Stochastic Optical Reconstruction Microscopy or dSTORM, one of the localisation super-resolution fluorescence microscopy techniques that are founded on the detection of fluorescence emissions from single molecules. We detail how, with minimal assemblage, a highly functional and versatile dSTORM set-up can be built from ‘off-the-shelf’ components at quite a modest budget, especially when compared with the current cost of commercial systems. We also present some typical super-resolution images of microtubules and actin filaments within cells and discuss sample preparation and labelling methods.

scholarly journals From 2D to 3D super-resolution imaging through glass microspheres -INVITED

2020 ◽  
Vol 238 ◽  
pp. 06002
Author(s):  
Stephane Perrin ◽  
Sylvain Lecler ◽  
Paul Montgomery
Keyword(s):  
3D Reconstruction ◽  
Imaging Technique ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Label Free ◽  
Magnification Factor ◽  
Glass Microspheres ◽  
Full Field ◽  
Resolution Imaging ◽  
2D To 3D

Microsphere-assisted microscopy is a new imaging technique which allows the diffraction limit to be overcome using transparent microspheres. It makes it possible to reach a resolution of up to 100 nm in air while being label-free and full-field. An overview of the imaging technique is presented showing the influence of the photonic jet on the image nature and the unconventional behaviour of the magnification factor. Moreover, interferometry through microspheres is demonstrated for the 3D reconstruction of nanoelements.

scholarly journals Recent Progress in the Correlative Structured Illumination Microscopy

Chemosensors ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 364
Author(s):  
Meiting Wang ◽  
Jiajie Chen ◽  
Lei Wang ◽  
Xiaomin Zheng ◽  
Jie Zhou ◽  
...  
Keyword(s):  
High Frequency ◽  
Optical Transmission ◽  
Imaging Technique ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Optical Diffraction ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Recent Developments ◽  
Resolution Imaging

The super-resolution imaging technique of structured illumination microscopy (SIM) enables the mixing of high-frequency information into the optical transmission domain via light-source modulation, thus breaking the optical diffraction limit. Correlative SIM, which combines other techniques with SIM, offers more versatility or higher imaging resolution than traditional SIM. In this review, we first briefly introduce the imaging mechanism and development trends of conventional SIM. Then, the principles and recent developments of correlative SIM techniques are reviewed. Finally, the future development directions of SIM and its correlative microscopies are presented.

scholarly journals Multilevel phase supercritical lens fabricated by synergistic optical lithography

Nanophotonics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1469-1477 ◽  
Author(s):  
Wei Fang ◽  
Jian Lei ◽  
Pengda Zhang ◽  
Fei Qin ◽  
Meiling Jiang ◽  
...  
Keyword(s):  
Numerical Aperture ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Optical Diffraction ◽  
Energy Concentration ◽  
Focal Region ◽  
Movement Trajectory ◽  
Label Free ◽  
Laser Fabrication ◽  
Resolution Imaging

AbstractThe advent of planar metalenses, including the super-oscillatory lens (SOL) and the supercritical lens (SCL) with distinctive interference properties, has profoundly impacted on the long-lasting perception of the far-field optical diffraction limit. In spite of its conspicuous success in achieving marvelously small focal spots, the planar metalens still faces tough design and fabrication challenges to realize high focusing efficiency. In this work, we demonstrated a dual-mode laser fabrication technique based on two-photon polymerization for realizing the multilevel phase SCL with focusing efficiency spiking. Synergistically controlling two types of movement trajectory, which is implemented with the piezo stage and the scanning galvo mirror, enables the fabrication of complicated structures with sub-diffraction-limit feature size. By utilizing such advantage, SCLs with discretized multilevel phase configurations are explicitly patterned. The experimental characterization results have shown that a four-level phase SCL can focus light into a sub-diffraction-limit spot with the lateral size of 0.41 λ/NA (NA is the numerical aperture), while achieve the focal spot intensity and the energy concentration ratio in the focal region 7.2 times and 3 times that of the traditional binary amplitude-type SCL with the same optimization conditions, respectively. Our results may release the application obstacles for the sub-diffraction-limit planar metalens and enable major advances in the fields from label-free optical super-resolution imaging to high precision laser fabrication.

scholarly journals Super-Resolution Imaging with Patchy Microspheres

Photonics ◽  
2021 ◽  
Vol 8 (11) ◽  
pp. 513
Author(s):  
Qingqing Shang ◽  
Fen Tang ◽  
Lingya Yu ◽  
Hamid Oubaha ◽  
Darwin Caina ◽  
...  
Keyword(s):  
Colloidal Particle ◽  
Near Field ◽  
Super Resolution ◽  
Incident Beam ◽  
Diffraction Limit ◽  
Beam Forming ◽  
Feature Size ◽  
Disc Surface ◽  
Promising Tool ◽  
Resolution Imaging

The diffraction limit is a fundamental barrier in optical microscopy, which restricts the smallest resolvable feature size of a microscopic system. Microsphere-based microscopy has proven to be a promising tool for challenging the diffraction limit. Nevertheless, the microspheres have a low imaging contrast in air, which hinders the application of this technique. In this work, we demonstrate that this challenge can be effectively overcome by using partially Ag-plated microspheres. The deposited Ag film acts as an aperture stop that blocks a portion of the incident beam, forming a photonic hook and an oblique near-field illumination. Such a photonic hook significantly enhanced the imaging contrast of the system, as experimentally verified by imaging the Blu-ray disc surface and colloidal particle arrays.

Sign in / Sign up

Export Citation Format

Share Document