scholarly journals Availability of lysine in protein concentrates as determined by the slope-ratio assay with chicks and comparisons with rat, pig and chemical assays

1981 ◽  
Vol 46 (3) ◽  
pp. 513-519 ◽  
Author(s):  
E. J. Major ◽  
E. S. Batterham
Keyword(s):  
Cottonseed Meal ◽  
Fish Meal ◽  
Basal Diet ◽  
Slope Ratio ◽  
Meat And Bone Meal ◽  
Sunflower Meal ◽  
Protein Concentrates ◽  
Available Lysine ◽  
Bean Meal ◽  
Soya Bean Meal

1. A slope-ratio assay was developed to determine the availability of lysine in protein concentrates for chicks, Two protein concentrates were assessed per assay, using three levels of incorporation into the basal diet.2. Availability of lysine, expressed as a proportion of total lysine in five protein concentrates was: cottonseed meal 0·83, fish meal 1·00, meat-and-bone meal 0·86, soya-bean meal 0·93, sunflower meal 1·01.3. The five protein concentrates had previously been assayed for available lysine with slope-ratio assays for pies and rats (Batterham et al. 1979; Batterharn et al. 1981), There was little relationship between the results for chicks and those for pigs and rats. For pigs, availability estimates ranged from 0·43 for cottonseed meal to 0·89 for fish meal. For rats, availability estimates ranged from 0·49 for sunflower meal to 1·04 for fish meal.4. The results for chicks were in closer agreement with values obtained using the Silcock available-lysine assay (Roach et al. 1967) and the direct 1-fluoro-2,4-dinitrobenzene procedure (Carpenter, 1960). By contrast, there was litlle relationship between the chemical tests and results for pigs or rats.

scholarly journals Availability of lysine in vegetable protein concentrates as determined by the slope-ratio assay with growing pigs and rats and by chemical techniques

1984 ◽  
Vol 51 (1) ◽  
pp. 85-99 ◽  
Author(s):  
E. S. Batterham ◽  
R. D. Murison ◽  
L. M. Andersen
Keyword(s):  
Cottonseed Meal ◽  
Vegetable Protein ◽  
Soya Bean ◽  
Growing Pigs ◽  
Slope Ratio ◽  
Protein Concentrates ◽  
Field Peas ◽  
Available Lysine ◽  
Bean Meal ◽  
Soya Bean Meal

1. The availability of lysine in nine vegetable-protein concentrates was assessed using the slope-ratio assay for growing pigs and rats. Diets were equalized for crude fibre using solka floc to minimize any possible effects of variation in fibre content on availability estimates.2. The availability of lysine in the nine proteins for pigs, using food conversion efficiency (FCE) on a carcass basis as the criterion of response were (proportion of total): cottonseed meal 0.39, lupin (Lupinus angustifolius) seed meal no. 10.37, no. 20.65, no. 30.54, no. 40.54, field peas (Pisum sativum) 0.93, peanut (groundnut) meal 0.57, soya-bean meal no. 10.98, no. 2 0.89.3. Estimates of available lysine for rats as assessed by the slope-ratio assay using FCE on a carcass basis were in close agreement with the pig estimates for cottonseed meal (0.35) and soya-bean meal no. 1(0.91) and no. 2(0.89), higher for lupin-seed meals (range 0.70–0.94 with a mean of 0.81) and peanut meal (0.76) and lower for field peas (0.76).4. The differences in available lysine were not detected by the chemical Silcock available-lysine test (Roach et al. 1967) or by the direct 1-fluoro-2,4-dinitrobenzene procedure (Carpenter, 1960).

scholarly journals Utilization of ileal digestible amino acids by growing pigs: methionine

1993 ◽  
Vol 70 (3) ◽  
pp. 711-720 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen ◽  
D. R. Baigent
Keyword(s):  
Cottonseed Meal ◽  
Soya Bean ◽  
Growing Pigs ◽  
Considerable Proportion ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Protein Concentrates ◽  
Bean Meal ◽  
Soya Bean Meal

An experiment was conducted to determine the utilization of ileal digestible methionine by growing pigs. Three methionine-deficient diets (0·09 g ileal digestible methionine/MJ digestible energy (DE)) were formulated using cottonseed meal, meat-and-bone meal and soya-bean meal respectively as the only source of methionine in the diet. An additional three diets were formulated with supplements of methionine to confirm that methionine was limiting in the first three diets. The growth performance and retention of methionine by pigs given the six diets over the 20–45 kg growth phase was then determined. Growth rates (g/d) of pigs given the three diets formulated to 0·09 g ileal digestible methionine/MJ DE were significantly different (P < 0·01): cottonseed meal 411, meat-and-bone meal 442, soya-bean meal 496 (SED 24·6). The response of pigs to the addition of methionine confirmed that methionine was limiting in these diets. Crude protein (N × 6·25) deposited by the pigs (g/d) was significantly higher (P < 0·05) for those given soya-bean meat (61) and meat-and-bone meal (57) relative io cottonseed meal (47; SED 3·3). The proportion of ileal digestible methionine retained by pigs given the three protein concentrates was: cottonseed meal 0·39, meat-and-bone meal 0·45, soya-bean meal 0·47 (SED 0·019). These results indicate that values for the ileal digestibility of methionine in protein concentrates do not reflect the proportion of methionine that can be utilized by the pig. It appears that, with heat-processed meals, a considerable proportion of the methionine is absorbed in a form(s) that is (are) inefficiently utilized.

Nutrition of the bacon pig XVI. The relative supplemental value of the proteins in extracted soya-bean meal and in white-fish meal

1952 ◽  
Vol 42 (4) ◽  
pp. 438-453 ◽  
Author(s):  
R. E. Evans
Keyword(s):  
Vegetable Protein ◽  
Nutritive Value ◽  
Fish Meal ◽  
Basal Diet ◽  
Soya Bean ◽  
Protein Concentrates ◽  
Vegetable Proteins ◽  
Bean Meal ◽  
The Subject ◽  
Soya Bean Meal

In continuation of our work on the relative supplemental value of animal and vegetable protein concentrates (Woodman & Evans, 1951), a study has been made of the proteins in extracted soya-bean meal. It is probable that vegetable protein concentrates may vary among themselves in nutritive value and that the proteins of ground-nut meal, which were the subject of investigation in our previous experiments, could not for this reason be regarded as representative of vegetable proteins in general. The same basal diet, composed of roughly 2 parts of barley meal and 1 part of fine bran, together with a little lucerne meal and minerals, was again used in the present investigation.

scholarly journals A comparison of the availability and ileal digestibility of lysine in cottonseed and soya-bean meals for grower/finisher pigs

1990 ◽  
Vol 64 (3) ◽  
pp. 663-677 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen ◽  
D. R. Baigent ◽  
R. E. Darnell ◽  
M. R. Taverner
Keyword(s):  
Amino Acids ◽  
Cottonseed Meal ◽  
Soya Bean ◽  
High Availability ◽  
Slope Ratio ◽  
Ileal Digestibility ◽  
Lysine Concentration ◽  
Available Lysine ◽  
Bean Meal ◽  
Soya Bean Meal

The availability of lysine and the ileal digestibility of amino acids in three cottonseed meals and a soya-bean meal for grower/finisher pigs were determined. The usefulness of the availability estimates for formulating diets was assessed. The availability of lysine, as assessed with a slope-ratio assay, was (proportion of total): cottonseed meal no. 1, 0.27; no. 2, 0.30, no. 3, 0.29; soya-bean meal, 0.90. Heal digestibility of lysine in the meals (proportion of total) was: cottonseed meal no. 1, 0.58; no. 2, 0.68; no. 3, 0.72; soya-bean meal, 0.89. Pigs given diets formulated to the same available lysine concentration grew at similar rates and retained the same amount of lysine in the carcasses. The results indicate that, for meals of high availability (soya-bean meal), reduced ileal digestibility appears to be the main reason for reduced availability. However, in meals of low availability (cottonseed meal), reduced ileal digestibility only accounts for part of the reduced availability. Thus, the ileal digestibility of lysine is not a reliable indicator of lysine availability.

scholarly journals Utilization of ileal digestible amino acids by pigs: Lysine

1990 ◽  
Vol 64 (3) ◽  
pp. 679-690 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen ◽  
D. R. Baigent ◽  
S. A. Beech ◽  
R. Elliott
Keyword(s):  
Amino Acids ◽  
Cottonseed Meal ◽  
Soya Bean ◽  
Considerable Proportion ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Protein Concentrates ◽  
Bean Meal ◽  
Soya Bean Meal

Two experiments were conducted to determine the utilization of ileal digestible lysine by pigs. In the first, the apparent ileal digestibility of amino acids in cottonseed meal, meat-and-bone meal and soya-bean meal was determined in pigs fitted with‘T'-shaped cannulas. In the second experiment, three lysine-deficient diets were formulated to 0.36 g ileal digestible lysine/MJ digestible energy (DE), with lysine contributed from the three protein concentrates as the only source of lysine in sugar-based diets. An additional three diets were formulated with supplements of lysine to verify that lysine was limiting in the first three diets. The growth performance and retention of lysine by pigs given the six diets over the 20–45 kg growth phase were then determined. The apparent ileal digestibility of lysine in the three protein concentrates (proportion of total) was: cottonseed meal 0.74, meat-and-bone meal 0.78, soya-bean meal 0.89. Growth rates (g/d) of the pigs given the three diets formulated to 0.36 g ileal digestible lysine/MJ DE were significantly different (P < 0.001): cottonseed meal 377, meat-and-bone meal 492, soya-bean meal 541. The response of pigs to the addition of lysine confirmed that lysine was limiting in these diets. Crude protein (nitrogen× 6.25) deposited by the pigs was significantly higher (P < 0.001) for those given soya-bean meal (77 g/d), relative to meat-and-bone meal (66 g/d) and cottonseed meal (38 g/d). The proportion of ileal digestible lysine retained by pigs given the three protein concentrates was: cottonseed meal 0.36, meat-and-bone meal 0.60, soya-bean meal 0.75. The results indicate that values for the ileal digestibility of lysine in protein concentrates are unsuitable in dietary formulations as the assay does not reflect the proportion of lysine that can be utilized by the pig. It appears that, with heat-processed meals, a considerable proportion of the lysine is absorbed in a form(s) that is (are) inefficiently utilized.

scholarly journals Utilization of ileal digestible amino acids by growing pigs: threonine

1991 ◽  
Vol 65 (3) ◽  
pp. 381-390 ◽  
Author(s):  
S. A. Beech ◽  
E. S. Batterham ◽  
R. Elliott
Keyword(s):  
Cottonseed Meal ◽  
Soya Bean ◽  
Growing Pigs ◽  
Considerable Proportion ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Protein Nitrogen ◽  
Protein Concentrates ◽  
Bean Meal ◽  
Soya Bean Meal

An experiment was conducted to determine the utilization of ileal digestible threonine by growing pigs. Three threonine-deficient diets (0.22 g ileal digestible threonine/MJ digestible energy (DE)) were formulated using cottonseed meal, meat-and-bone meal and soya-bean meal respectively, as the only source of threonine in the diet. An additional three diets were formulated with supplements of threonine to confirm that threonine was limiting in the first three diets. The growth performance and retention of threonine by pigs given the six diets over the 20–45 kg growth phase was then determined. Growth rates (g/d) of the pigs given the three diets formulated to 0.22 g ileal digestible threonine/MJ DE were significantly different (P < 0.001): cottonseed meal 417, meat-and-bone meal 452, soya-bean meal 524 (sed 13.6). The response of pigs to the addition of threonine confirmed that threonine was limiting in these diets. Crude protein (nitrogen x 6.25) deposited by the pigs (g/d) was significantly higher (P < 0.001) for those given soya-bean meal (75), relative to meat-and-bone meal (62) and cottonseed meal (47) (sed 3.3). The proportion of ileal digestible threonine retained by pigs given the three protein concentrates was: cottonseed meal 0.44, meat-and-bone meal 0.59, soya-bean meal 0.64 (sed 0.024). These results indicate that values for the ileal digestibility of threonine in protein concentrates are unsuitable in dietary formulations as the assay does not reflect the proportion of threonine that can be utilized by the pig. It appears that, with heat-processed meals, a considerable proportion of the threonine is absorbed in a form(s) that is (are) inefficiently utilized.

Intensive beef production 5. The effect of different sources of protein on nitrogen retention

1965 ◽  
Vol 7 (3) ◽  
pp. 303-308 ◽  
Author(s):  
H. B. Bowers ◽  
T. R. Preston ◽  
N. A. MacLeod ◽  
I. McDonald ◽  
Euphemia B. Philip
Keyword(s):  
Cottonseed Meal ◽  
Dry Matter ◽  
Fish Meal ◽  
Nitrogen Retention ◽  
Soya Bean ◽  
Dry Matter Intake ◽  
Voluntary Feed Intake ◽  
Bean Meal ◽  
Soya Bean Meal ◽  
Different Sources

SUMMARY1. Nitrogen balance studies were made on 8 Friesian steers given allconcentrate diets containing fish meal, soya bean meal, groundnut meal or cottonseed meal.2. There were no differences in nitrogen retention but voluntary feed intake was significantly lower on the fish meal diet.3. Adjustment of nitrogen retention to constant dry matter intake resulted in significantly higher values for the diets containing fish meal and cottonseed meal than for the others.4. Rumen pH, rumen ammonia and blood urea were all much lower on the fish meal diet.

Digestibility of threonine from protein concentrates for growing pigs. 2. The digestibility of threonine to the terminal ileum in pigs given six protein concentrates

1988 ◽  
Vol 39 (4) ◽  
pp. 721 ◽  
Author(s):  
J Leibholz ◽  
Y Mollah
Keyword(s):  
Cottonseed Meal ◽  
Soybean Meal ◽  
Terminal Ileum ◽  
Dry Matter ◽  
Fish Meal ◽  
Growing Pigs ◽  
Apparent Digestibility ◽  
Sunflower Meal ◽  
Protein Concentrates ◽  
Meat Meal

Six male Landrace x Large White pigs (25 kg liveweight) were fitted with T-shape cannulae in the terminal ileum. The pigs were fed 1.2 kg dry matter per day containing 4.5 g threonine/kg of diet from continuous belt feeders. The apparent digestibility of dry matter (DM) to the terminal ileum was 0.91, 0.87, 0.85, 0.81, 0.80 and 0.75 for pigs given diets containing milk, fish meal, soybean meal, meat meal, sunflower meal and cotton seed meal respectively. The apparent digestibility of nitrogen (N) to the terminal ileum was 0.87, 0.87, 0.86, 0.86, 0.81 and 0.74 for pigs given diets containing milk, fish meal, soybean meal, sunflower meal, meat meal and cottonseed meal, respectively. The true digestibility of threonine to the terminal ileum was 0.96, 0.95, 0.90, 0.87, 0.80 and 0.66 for the diets containing milk, fish meal, sunflower meal, soybean meal, meat meal and cottonseed meal, respectively.

A note on the effect of amino acid or urea supplements to barley diets for the finishing pig

1969 ◽  
Vol 11 (4) ◽  
pp. 553-556 ◽  
Author(s):  
H. F. Hintz ◽  
W. G. Pond ◽  
W. J. Visek
Keyword(s):  
Growth Rate ◽  
Amino Acid ◽  
Protein Content ◽  
Cottonseed Meal ◽  
Carcass Characteristics ◽  
Basal Diet ◽  
Soya Bean ◽  
Finishing Pig ◽  
Bean Meal ◽  
Soya Bean Meal

SUMMARYIn trials 1 and 2, supplements of urea and cottonseed meal increased the protein content of the basal diets from 12·2 to 14·0–14·6%, but had no significant effects on growth rate or carcass characteristics. In trial 3 the effect of cottonseed meal on growth rate was almost significant but urea had less effect. In trial 4, the basal diet contained 11·0% protein; soya bean meal increased this to 13·1% and had an almost significant effect on growth rate. Lysine and lysine + urea had no effect.

Utilization of ileal digestible amino acids by growing pigs: isoleucine

1994 ◽  
Vol 71 (4) ◽  
pp. 531-541 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen
Keyword(s):  
Amino Acids ◽  
Cottonseed Meal ◽  
Soya Bean ◽  
Growing Pigs ◽  
Seed Meal ◽  
Ileal Digestibility ◽  
Protein Concentrates ◽  
Lupin Seed ◽  
Bean Meal ◽  
Soya Bean Meal

Two experiments were conducted to determine the utilization of ileal digestible isoleucine by growing pigs. In the first, the apparent ileal digestibility of amino acids in cottonseed meal, lupin-seed meal and soya-bean meal was determined in pigs fitted with‘T‘-shaped cannulas. In the second, three isoleucine-deficient diets were formulated to 0.23 g ileal digestible isoleucine/MJ digestible energy (DE) with the three protein concentrates contributing the only source of isoleucine in sucrose-based diets. An additional three diets were formulated with supplements of isoleucine to confirm that isoleucine was limiting in the first three diets. The growth performance and retention of isoleucine by pigs given the six diets over the 20–45 kg growth phase were then determined. The apparent ileal digestibility of isoleucine in the three protein concentrates (proportion of total) was: cottonseed meal 0.68, lupin-seed meal 0.86, soya-bean meal 0.86. There were no significant differences (P > 0.05) in growth rates (g/d) and crude protein deposition rates (g/d) of the pigs given the three diets formulated to 0.23 g ileal digestible isoleucine/MJ DE: cottonseed meal 590, 84; lupin-seed meal 613, 87; soya-bean meal 594, 91 (SEM 13.0, 2.9) respectively. The response of pigs to the addition of isoleucine confirmed that isoleucine was limiting in these diets. The proportion of ileal digestible isoleucine retained by pigs given the cottonseed meal (0.65) was slightly lower than that retained by pigs given soya-bean meal (0.73; P < 0.05). These results indicate that values for the ileal digestibility of isoleucine in protein concentrates more closely reflect the proportion of isoleucine that can be utilized by the pig than occurs for other amino acids such as lysine, threonine and methionine.

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