Three-dimensional cell culture of chondrocytes on modified di-phenylalanine scaffolds

2007 ◽  
Vol 35 (3) ◽  
pp. 535-537 ◽  
Author(s):  
V. Jayawarna ◽  
A. Smith ◽  
J.E. Gough ◽  
R.V. Ulijn
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Cell Proliferation ◽  
Self Assembly ◽  
Three Dimensional ◽  
Building Blocks ◽  
Culture Conditions ◽  
The Self ◽  
Three Dimensions ◽  
Dimensional Cell

The design of self-assembled peptide-based structures for three-dimensional cell culture and tissue repair has been a key objective in biomaterials science for decades. In search of the simplest possible peptide system that can self-assemble, we discovered that combinations of di-peptides that are modified with aromatic stacking ligands could form nanometre-sized fibres when exposed to physiological conditions. For example, we demonstrated that a number of Fmoc (fluoren-9-ylmethyloxycarbonyl) modified di- and tri-peptides form highly ordered hydrogels via hydrogen-bonding and π–π interactions from the fluorenyl rings. These highly hydrated gels allowed for cell proliferation of chondrocytes in three dimensions [Jayawarna, Ali, Jowitt, Miller, Saiani, Gough and Ulijn (2006) Adv. Mater. 18, 611–614]. We demonstrated that fibrous architecture and physical properties of the resulting materials were dictated by the nature of the amino acid building blocks. Here, we report the self-assembly process of three di-phenylalanine analogues, Fmoc-Phe-Phe-OH, Nap (naphthalene)-Phe-Phe-OH and Cbz (benzyloxycarbonyl)-Phe-Phe-OH, to compare and contrast the self-assembly properties and cell culture conditions attributable to their protecting group difference. Fibre morphology analysis of the three structures using cryo-SEM (scanning electron microscopy) and TEM (transmission electron microscopy) suggested fibrous structures with dramatically varying fibril dimensions, depending on the aromatic ligand used. CD and FTIR (Fourier-transform IR) data confirmed β-sheet arrangements in all three samples in the gel state. The ability of these three new hydrogels to support cell proliferation of chondrocytes was confirmed for all three materials.

Polypeptide-engineered physical hydrogels designed from the coiled-coil region of cartilage oligomeric matrix protein for three-dimensional cell culture

2014 ◽  
Vol 2 (20) ◽  
pp. 3123-3132 ◽  
Author(s):  
Ming-Hao Yao ◽  
Jie Yang ◽  
Ming-Shuo Du ◽  
Ji-Tao Song ◽  
Yong Yu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Self Assembly ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Three Dimensional ◽  
Coiled Coil ◽  
The Self ◽  
Coiled Coil Region ◽  
Dimensional Cell

A class of physical hydrogels photo-cross-linked from multi-branched photopolymeriized monomers based on the self-assembly of coiled-coil polypeptide P is developed.

A Model of Force Generation in a Three-Dimensional Toroidal Cluster of Cells

2012 ◽  
Vol 79 (5) ◽  
Author(s):  
Asha Nurse ◽  
L. B. Freund ◽  
Jacquelyn Youssef
Keyword(s):  
Potential Energy ◽  
Gravitational Potential ◽  
Self Assembly ◽  
Three Dimensional ◽  
Dissipative System ◽  
The Self ◽  
Three Dimensions ◽  
Force Generation ◽  
Gravitational Potential Energy ◽  
Kinetics Of

Observation of the self-assembly of clusters of cells in three dimensions has raised questions about the forces that drive changes in the shape of the cell clusters. Cells that self-assemble into a toroidal cluster about the base of a conical pillar have been observed in the laboratory to spontaneously climb the conical pillar. Assuming that cell cluster reorganization is due solely to surface diffusion, a mathematical model based on the thermodynamics of an isothermal dissipative system is presented. The model shows that the cluster can reduce its surface area by climbing the conical pillar, however, this is at the expense of increasing its gravitational potential energy. As a result, the kinetics of the climb are affected by parameters that influence this energy competition, such as the slope of the conical pillar and a parameter of the model κ that represents the influence of the surface energy of the cluster relative to its gravitational potential energy.

scholarly journals Cell proliferation inin vivo-like three-dimensional cell culture is regulated by sequestration of ERK1/2 to lipid rafts

2014 ◽  
Vol 47 (4) ◽  
pp. 336-346 ◽  
Author(s):  
R. Skrobanska ◽  
A. Evangelatov ◽  
N. Stefanova ◽  
T. Topouzova-Hristova ◽  
A. Momchilova ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
Lipid Rafts ◽  
Three Dimensional ◽  
Dimensional Cell

scholarly journals Protein Fibrillar Hydrogels for three-Dimensional Tissue Engineering

2009 ◽  
Vol 2009 ◽  
pp. 1-4 ◽  
Author(s):  
Hui Yan ◽  
Antti Nykanen ◽  
Janne Ruokolainen ◽  
David Farrar ◽  
Aline F. Miller
Keyword(s):  
Cell Culture ◽  
Self Assembly ◽  
Culture Media ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Disease States ◽  
Environmental Responsiveness ◽  
Transmission Electron ◽  
Biological Compatibility ◽  
Oscillatory Rheology

Protein self-assembly into highly ordered fibrillar aggregates has attracted increasing attention over recent years, due primarily to its association with disease states such as Alzheimer's. More recently, however, research has focused on understanding the generic behavior of protein self-assembly where fibrillation is typically induced under harsh conditions of low pH and/or high temperature. Moreover the inherent properties of these fibrils, including their nanoscale dimension, environmental responsiveness, and biological compatibility, are attracting substantial interest for exploiting these fibrils for the creation of new materials. Here we will show how protein fibrils can be formed under physiological conditions and their subsequent gelation driven using the ionic strength of cell culture media while simultaneously incorporating cells homogeneously throughout the gel network. The fibrillar and elastic nature of the gel have been confirmed using cryo-transmission electron microscopy and oscillatory rheology, respectively; while cell culture work shows that our hydrogels promote cell spreading, attachment, and proliferation in three dimensions.

scholarly journals Frustrated self-assembly of non-Euclidean crystals of nanoparticles

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Francesco Serafin ◽  
Jun Lu ◽  
Nicholas Kotov ◽  
Kai Sun ◽  
Xiaoming Mao
Keyword(s):  
Self Assembly ◽  
Three Dimensional ◽  
Building Blocks ◽  
The Self ◽  
High Yield ◽  
Complex Structures ◽  
Interparticle Interactions ◽  
Viral Capsids ◽  
Self Organized ◽  
New Material

AbstractSelf-organized complex structures in nature, e.g., viral capsids, hierarchical biopolymers, and bacterial flagella, offer efficiency, adaptability, robustness, and multi-functionality. Can we program the self-assembly of three-dimensional (3D) complex structures using simple building blocks, and reach similar or higher level of sophistication in engineered materials? Here we present an analytic theory for the self-assembly of polyhedral nanoparticles (NPs) based on their crystal structures in non-Euclidean space. We show that the unavoidable geometrical frustration of these particle shapes, combined with competing attractive and repulsive interparticle interactions, lead to controllable self-assembly of structures of complex order. Applying this theory to tetrahedral NPs, we find high-yield and enantiopure self-assembly of helicoidal ribbons, exhibiting qualitative agreement with experimental observations. We expect that this theory will offer a general framework for the self-assembly of simple polyhedral building blocks into rich complex morphologies with new material capabilities such as tunable optical activity, essential for multiple emerging technologies.

Engineering ellipsoidal cap-like hydrogel particles as building blocks or sacrificial templates for three-dimensional cell culture

2018 ◽  
Vol 6 (4) ◽  
pp. 885-892 ◽  
Author(s):  
Weiwei Zhang ◽  
Guoyou Huang ◽  
Kelvin Ng ◽  
Yuan Ji ◽  
Bin Gao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Building Blocks ◽  
Aao Template ◽  
Hydrogel Particles ◽  
Dimensional Cell

AAO template-assisted fabrication of ellipsoidal cap-like hydrogel particles as building blocks or sacrificial templates for 3D cell culture.

scholarly journals Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing

2020 ◽  
Vol 7 (3) ◽  
pp. 115
Author(s):  
Patrick Bédard ◽  
Sara Gauvin ◽  
Karel Ferland ◽  
Christophe Caneparo ◽  
Ève Pellerin ◽  
...  
Keyword(s):  
Self Assembly ◽  
Three Dimensional ◽  
The Self ◽  
Two Dimensional ◽  
Animal Testing ◽  
Accurate Knowledge ◽  
Biological Phenomena ◽  
Organ Specific ◽  
Three Dimensional Models ◽  
Dimensional Cell

Animal testing has long been used in science to study complex biological phenomena that cannot be investigated using two-dimensional cell cultures in plastic dishes. With time, it appeared that more differences could exist between animal models and even more when translated to human patients. Innovative models became essential to develop more accurate knowledge. Tissue engineering provides some of those models, but it mostly relies on the use of prefabricated scaffolds on which cells are seeded. The self-assembly protocol has recently produced organ-specific human-derived three-dimensional models without the need for exogenous material. This strategy will help to achieve the 3R principles.

Nanostructured Hydrogels for Three-Dimensional Cell Culture Through Self-Assembly of Fluorenylmethoxycarbonyl–Dipeptides

2006 ◽  
Vol 18 (5) ◽  
pp. 611-614 ◽  
Author(s):  
V. Jayawarna ◽  
M. Ali ◽  
T. A. Jowitt ◽  
A. F. Miller ◽  
A. Saiani ◽  
...  
Keyword(s):  
Cell Culture ◽  
Self Assembly ◽  
Three Dimensional ◽  
Dimensional Cell
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