Engineering ellipsoidal cap-like hydrogel particles as building blocks or sacrificial templates for three-dimensional cell culture

2018 ◽  
Vol 6 (4) ◽  
pp. 885-892 ◽  
Author(s):  
Weiwei Zhang ◽  
Guoyou Huang ◽  
Kelvin Ng ◽  
Yuan Ji ◽  
Bin Gao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Building Blocks ◽  
Aao Template ◽  
Hydrogel Particles ◽  
Dimensional Cell

AAO template-assisted fabrication of ellipsoidal cap-like hydrogel particles as building blocks or sacrificial templates for 3D cell culture.

Study on the Toxicity of Calix[4]- and [6]-Arenes and their Sulfated Derivatives in Two- and Three-Dimensional Cell Cultures of Colorectal Adenocarcinoma

2021 ◽  
Vol 37 (1) ◽  
pp. 87-94
Author(s):  
S.V. Nikulin ◽  
B.Ya. Alekseev ◽  
A.N Gorbunov ◽  
I.M. Vatsuro ◽  
V.V. Kovalev ◽  
...  
Keyword(s):  
Cell Culture ◽  
Colorectal Adenocarcinoma ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Russian Science ◽  
Adenocarcinoma Cells ◽  
Therapeutic Molecules ◽  
Derivatives Of ◽  
Ht 29 ◽  
Dimensional Cell

A comparative study of the toxicity of two unsubstituted calixarenes consisting of 4 and 6 phenolic fragments, as well as their p-sulfated derivatives, was carried out on the HT-29 colorectal adenocarcinoma cells cultured in two-dimensional (2D) and three-dimensional (3D) formats. It was shown that both unsubstituted calixarenes decrease the viability of tumor cells; calix[4]arene and calix[6]arene exhibited a cytostatic and a cytotoxic effect, respectively. Sulfated derivatives of calixarenes did not have a pronounced toxic effect on HT-29 cells. However, due to their high hydrophilicity and the ability to form adducts with various therapeutic molecules, they can be used for delivery of anticancer drugs. calixarenes, cytotoxicity, HT-29 cells, 2D cell culture, 3D cell culture The work was financially supported by the Russian Science Foundation (project no. 19-15-00397).

Leaf-inspired artificial microvascular networks (LIAMN) for three-dimensional cell culture

RSC Advances ◽  
2015 ◽  
Vol 5 (110) ◽  
pp. 90596-90601 ◽  
Author(s):  
Rong Fan ◽  
Yihang Sun ◽  
Jiandi Wan
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Microvascular Networks ◽  
Dimensional Cell

Leaf-inspired artificial microvascular networks (LIAMN) for 3D cell culture in hydrogel constructs.

scholarly journals Cytotoxicity Profile of Endodontic Sealers Provided by 3D Cell Culture Experimental Model

2016 ◽  
Vol 27 (6) ◽  
pp. 652-656 ◽  
Author(s):  
Emmanuel João Nogueira Leal Silva ◽  
Nancy Kudsi de Carvalho ◽  
Carina Taboada Ronconi ◽  
Gustavo De-Deus ◽  
Mario Luis Zuolo ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Type I ◽  
Cell Culture Model ◽  
Cytotoxic Effects ◽  
Culture Model ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
Dimensional Cell

Abstract The aim of the present study was to evaluate the cytotoxic effects of five endodontic sealers (AH Plus, Endomethasone N, EndoSequence BC, MTA Fillapex and Pulp Canal Sealer EWT) using a three-dimensional (3D) cell culture model. A conventional bi-dimensional (2D) cell culture model was used as reference technique for comparison. Balb/c 3T3 fibroblasts were cultured in conventional bi-dimensional cell culture and in rat-tail collagen type I three-dimensional cell culture models. Then, both cell cultures were incubated with elutes of freshly mixed endodontic sealers for 24 h. Cell viability was measured by the methyl-thiazol-diphenyltetrazolium assay (MTT). Data were statistically analyzed using ANOVA and the Tukey test at a significance level of p<0.05. All tested sealers exhibited cytotoxic effects; however, cytotoxic effect was culture model- and sealer-dependent. Sealers showed higher cytotoxicity in 2D than in 3D cell culture model (p<0.05). In both conditions, EndoSequence BC showed the lowest cytotoxicity (p<0.05). MTA Fillapex was much more cytotoxic than the other tested endodontic sealers (p<0.05), with the exception of AH Plus in the 2D cell culture model (p>0.05). Endomethasone N and Pulp Canal Sealer EWT showed lower cytotoxic effects than AH Plus in 2D cell culture model (p<0.05); however no statistical differences was observed among these sealers in 3D cell culture model. It may be concluded that cytotoxicity was higher in 2D cell culture compared to 3D cell culture. EndoSequence BC sealer exhibited the highest cytocompatibility and MTA Fillapex the lowest cytocompatibility.

scholarly journals Use of electrospinning and dynamic air focusing to create three-dimensional cell culture scaffolds in microfluidic devices

The Analyst ◽  
2016 ◽  
Vol 141 (18) ◽  
pp. 5311-5320 ◽  
Author(s):  
Chengpeng Chen ◽  
Benjamin T. Mehl ◽  
Scott A. Sell ◽  
R. Scott Martin
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Microfluidic Devices ◽  
Dimensional Cell

An air focusing technique was used to directly electrospin fibers into fully sealed microfluidic devices for 3D cell culture.

scholarly journals Three-dimensional cell culture, opportunities and challenges for bioprocess engineers

2016 ◽  
Vol 3 (6) ◽  
pp. 263-277
Author(s):  
Samille Henriques Pereira ◽  
Denise Soares de Moura Coutinho ◽  
Ana Flávia de Oliveira Gonçalves de Matos ◽  
Willer Ferreira da Silva Junior ◽  
Daniela Leite Fabrino
Keyword(s):  
Cell Culture ◽  
Life Science ◽  
New Technologies ◽  
Scale Up ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Cellular Heterogeneity ◽  
Multicellular Spheroids ◽  
Phenotypic Characteristics ◽  
Dimensional Cell

Two-dimensional cell culture (2D) is the most used technique in studies of mass production of proteins and vaccines; however, this technique is quite limited, since cells lose their phenotypic characteristics when cultured in monolayer. As an alternative, three-dimensional cell culture (3D) allowed cells to be cultured within an environment closer to their natural one, keeping in that way, their physiologic characteristics. When grown in this kind of system, cells form structures called multicellular spheroids, which present in their cores: cellular heterogeneity, microenvironment formation, and different expositions to several factors, such as nutrients and oxygen. This technique has revolutionized researches on drug development and its mechanism of action, since the results obtained in 3D cell culture are more realistic than the ones arisen from 2D cell culture. Recently, there have been developed many 3D cell culture methodologies, however, it misses technology to scale up the biomass growth, which is a great challenge for bioprocess engineers (BE). Therefore, this review aimed to show the technical reality of 3D cell culture and how such professionals can apply their engineering and life science knowledge to improve and develop new technologies that make the use of 3D cell culture feasible and widely used by biotechnological industries.

scholarly journals Scaffold-free three-dimensional cell culturing using magnetic levitation

2018 ◽  
Vol 6 (7) ◽  
pp. 1745-1753 ◽  
Author(s):  
Esra Türker ◽  
Nida Demirçak ◽  
Ahu Arslan-Yildiz
Keyword(s):  
Cell Culture ◽  
Magnetic Levitation ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
3D Structures ◽  
Cell Culturing ◽  
Culture Formation ◽  
Dimensional Cell

Magnetic levitation platform ensures a scaffold-free 3D cell culture formation by utilizing Gadolinium(iii) chelates, which provide paramagnetic environment for levitation; therefore, the cells are assembled into complex 3D structures.

Three-dimensional cell culture of chondrocytes on modified di-phenylalanine scaffolds

2007 ◽  
Vol 35 (3) ◽  
pp. 535-537 ◽  
Author(s):  
V. Jayawarna ◽  
A. Smith ◽  
J.E. Gough ◽  
R.V. Ulijn
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Cell Proliferation ◽  
Self Assembly ◽  
Three Dimensional ◽  
Building Blocks ◽  
Culture Conditions ◽  
The Self ◽  
Three Dimensions ◽  
Dimensional Cell

The design of self-assembled peptide-based structures for three-dimensional cell culture and tissue repair has been a key objective in biomaterials science for decades. In search of the simplest possible peptide system that can self-assemble, we discovered that combinations of di-peptides that are modified with aromatic stacking ligands could form nanometre-sized fibres when exposed to physiological conditions. For example, we demonstrated that a number of Fmoc (fluoren-9-ylmethyloxycarbonyl) modified di- and tri-peptides form highly ordered hydrogels via hydrogen-bonding and π–π interactions from the fluorenyl rings. These highly hydrated gels allowed for cell proliferation of chondrocytes in three dimensions [Jayawarna, Ali, Jowitt, Miller, Saiani, Gough and Ulijn (2006) Adv. Mater. 18, 611–614]. We demonstrated that fibrous architecture and physical properties of the resulting materials were dictated by the nature of the amino acid building blocks. Here, we report the self-assembly process of three di-phenylalanine analogues, Fmoc-Phe-Phe-OH, Nap (naphthalene)-Phe-Phe-OH and Cbz (benzyloxycarbonyl)-Phe-Phe-OH, to compare and contrast the self-assembly properties and cell culture conditions attributable to their protecting group difference. Fibre morphology analysis of the three structures using cryo-SEM (scanning electron microscopy) and TEM (transmission electron microscopy) suggested fibrous structures with dramatically varying fibril dimensions, depending on the aromatic ligand used. CD and FTIR (Fourier-transform IR) data confirmed β-sheet arrangements in all three samples in the gel state. The ability of these three new hydrogels to support cell proliferation of chondrocytes was confirmed for all three materials.

scholarly journals Applications of Biomaterials in 3D Cell Culture and Contributions of 3D Cell Culture to Drug Development and Basic Biomedical Research

2021 ◽  
Vol 22 (5) ◽  
pp. 2491
Author(s):  
Yujin Park ◽  
Kang Moo Huh ◽  
Sun-Woong Kang
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
3D Cell Culture ◽  
Accurate Method ◽  
New Drugs ◽  
Toxicity Screening ◽  
Cellular Functions ◽  
Toxicity Of Drugs ◽  
External Stimuli

The process of evaluating the efficacy and toxicity of drugs is important in the production of new drugs to treat diseases. Testing in humans is the most accurate method, but there are technical and ethical limitations. To overcome these limitations, various models have been developed in which responses to various external stimuli can be observed to help guide future trials. In particular, three-dimensional (3D) cell culture has a great advantage in simulating the physical and biological functions of tissues in the human body. This article reviews the biomaterials currently used to improve cellular functions in 3D culture and the contributions of 3D culture to cancer research, stem cell culture and drug and toxicity screening.

scholarly journals Mammary gland 3D cell culture systems in farm animals

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Laurence Finot ◽  
Eric Chanat ◽  
Frederic Dessauge
Keyword(s):  
Cell Culture ◽  
Mammary Gland ◽  
Bacterial Infections ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Farm Animals ◽  
Culture Systems ◽  
Cell Culture Systems

AbstractIn vivo study of tissue or organ biology in mammals is very complex and progress is slowed by poor accessibility of samples and ethical concerns. Fortunately, however, advances in stem cell identification and culture have made it possible to derive in vitro 3D “tissues” called organoids, these three-dimensional structures partly or fully mimicking the in vivo functioning of organs. The mammary gland produces milk, the source of nutrition for newborn mammals. Milk is synthesized and secreted by the differentiated polarized mammary epithelial cells of the gland. Reconstructing in vitro a mammary-like structure mimicking the functional tissue represents a major challenge in mammary gland biology, especially for farm animals for which specific agronomic questions arise. This would greatly facilitate the study of mammary gland development, milk secretion processes and pathological effects of viral or bacterial infections at the cellular level, all with the objective of improving milk production at the animal level. With this aim, various 3D cell culture models have been developed such as mammospheres and, more recently, efforts to develop organoids in vitro have been considerable. Researchers are now starting to draw inspiration from other fields, such as bioengineering, to generate organoids that would be more physiologically relevant. In this chapter, we will discuss 3D cell culture systems as organoids and their relevance for agronomic research.

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

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