Polypeptide-engineered physical hydrogels designed from the coiled-coil region of cartilage oligomeric matrix protein for three-dimensional cell culture

2014 ◽  
Vol 2 (20) ◽  
pp. 3123-3132 ◽  
Author(s):  
Ming-Hao Yao ◽  
Jie Yang ◽  
Ming-Shuo Du ◽  
Ji-Tao Song ◽  
Yong Yu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Self Assembly ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Three Dimensional ◽  
Coiled Coil ◽  
The Self ◽  
Coiled Coil Region ◽  
Dimensional Cell

A class of physical hydrogels photo-cross-linked from multi-branched photopolymeriized monomers based on the self-assembly of coiled-coil polypeptide P is developed.

Three-dimensional cell culture of chondrocytes on modified di-phenylalanine scaffolds

2007 ◽  
Vol 35 (3) ◽  
pp. 535-537 ◽  
Author(s):  
V. Jayawarna ◽  
A. Smith ◽  
J.E. Gough ◽  
R.V. Ulijn
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Cell Proliferation ◽  
Self Assembly ◽  
Three Dimensional ◽  
Building Blocks ◽  
Culture Conditions ◽  
The Self ◽  
Three Dimensions ◽  
Dimensional Cell

The design of self-assembled peptide-based structures for three-dimensional cell culture and tissue repair has been a key objective in biomaterials science for decades. In search of the simplest possible peptide system that can self-assemble, we discovered that combinations of di-peptides that are modified with aromatic stacking ligands could form nanometre-sized fibres when exposed to physiological conditions. For example, we demonstrated that a number of Fmoc (fluoren-9-ylmethyloxycarbonyl) modified di- and tri-peptides form highly ordered hydrogels via hydrogen-bonding and π–π interactions from the fluorenyl rings. These highly hydrated gels allowed for cell proliferation of chondrocytes in three dimensions [Jayawarna, Ali, Jowitt, Miller, Saiani, Gough and Ulijn (2006) Adv. Mater. 18, 611–614]. We demonstrated that fibrous architecture and physical properties of the resulting materials were dictated by the nature of the amino acid building blocks. Here, we report the self-assembly process of three di-phenylalanine analogues, Fmoc-Phe-Phe-OH, Nap (naphthalene)-Phe-Phe-OH and Cbz (benzyloxycarbonyl)-Phe-Phe-OH, to compare and contrast the self-assembly properties and cell culture conditions attributable to their protecting group difference. Fibre morphology analysis of the three structures using cryo-SEM (scanning electron microscopy) and TEM (transmission electron microscopy) suggested fibrous structures with dramatically varying fibril dimensions, depending on the aromatic ligand used. CD and FTIR (Fourier-transform IR) data confirmed β-sheet arrangements in all three samples in the gel state. The ability of these three new hydrogels to support cell proliferation of chondrocytes was confirmed for all three materials.

N-Terminal Aliphatic Residues Dictate the Structure, Stability, Assembly, and Small Molecule Binding of the Coiled-Coil Region of Cartilage Oligomeric Matrix Protein

Biochemistry ◽  
2009 ◽  
Vol 48 (36) ◽  
pp. 8559-8567 ◽  
Author(s):  
Susheel K. Gunasekar ◽  
Mukta Asnani ◽  
Chandani Limbad ◽  
Jennifer S. Haghpanah ◽  
Wendy Hom ◽  
...  
Keyword(s):  
Small Molecule ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Coiled Coil ◽  
Structure Stability ◽  
Coiled Coil Region

scholarly journals Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing

2020 ◽  
Vol 7 (3) ◽  
pp. 115
Author(s):  
Patrick Bédard ◽  
Sara Gauvin ◽  
Karel Ferland ◽  
Christophe Caneparo ◽  
Ève Pellerin ◽  
...  
Keyword(s):  
Self Assembly ◽  
Three Dimensional ◽  
The Self ◽  
Two Dimensional ◽  
Animal Testing ◽  
Accurate Knowledge ◽  
Biological Phenomena ◽  
Organ Specific ◽  
Three Dimensional Models ◽  
Dimensional Cell

Animal testing has long been used in science to study complex biological phenomena that cannot be investigated using two-dimensional cell cultures in plastic dishes. With time, it appeared that more differences could exist between animal models and even more when translated to human patients. Innovative models became essential to develop more accurate knowledge. Tissue engineering provides some of those models, but it mostly relies on the use of prefabricated scaffolds on which cells are seeded. The self-assembly protocol has recently produced organ-specific human-derived three-dimensional models without the need for exogenous material. This strategy will help to achieve the 3R principles.

Nanostructured Hydrogels for Three-Dimensional Cell Culture Through Self-Assembly of Fluorenylmethoxycarbonyl–Dipeptides

2006 ◽  
Vol 18 (5) ◽  
pp. 611-614 ◽  
Author(s):  
V. Jayawarna ◽  
M. Ali ◽  
T. A. Jowitt ◽  
A. F. Miller ◽  
A. Saiani ◽  
...  
Keyword(s):  
Cell Culture ◽  
Self Assembly ◽  
Three Dimensional ◽  
Dimensional Cell

Self-Assembly of Dendritic DNA into a Hydrogel: Application in Three-Dimensional Cell Culture

2021 ◽  
Author(s):  
Jingyuan Wu ◽  
Bella Rosa Liyarita ◽  
Haishuang Zhu ◽  
Ming Liu ◽  
Xiao Hu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Self Assembly ◽  
Three Dimensional ◽  
Dimensional Cell

Nanoscale Self-Assembly Using Ion and Electron Beam Techniques: A Rapid Review

MRS Advances ◽  
2020 ◽  
Vol 5 (64) ◽  
pp. 3507-3520
Author(s):  
Chunhui Dai ◽  
Kriti Agarwal ◽  
Jeong-Hyun Cho
Keyword(s):  
Electron Beam ◽  
Self Assembly ◽  
Ion Beam ◽  
Three Dimensional ◽  
The Self ◽  
Stress Generation ◽  
Rapid Review ◽  
High Yield ◽  
3D Nanostructures ◽  
Self Assembled

AbstractNanoscale self-assembly, as a technique to transform two-dimensional (2D) planar patterns into three-dimensional (3D) nanoscale architectures, has achieved tremendous success in the past decade. However, an assembly process at nanoscale is easily affected by small unavoidable variations in sample conditions and reaction environment, resulting in a low yield. Recently, in-situ monitored self-assembly based on ion and electron irradiation has stood out as a promising candidate to overcome this limitation. The usage of ion and electron beam allows stress generation and real-time observation simultaneously, which significantly enhances the controllability of self-assembly. This enables the realization of various complex 3D nanostructures with a high yield. The additional dimension of the self-assembled 3D nanostructures opens the possibility to explore novel properties that cannot be demonstrated in 2D planar patterns. Here, we present a rapid review on the recent achievements and challenges in nanoscale self-assembly using electron and ion beam techniques, followed by a discussion of the novel optical properties achieved in the self-assembled 3D nanostructures.

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

A novel fluffy hydroxylapatite fiber scaffold with deep interconnected pores designed for three-dimensional cell culture

2014 ◽  
Vol 2 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Lin Jin ◽  
Zhang-Qi Feng ◽  
Ting Wang ◽  
Zhuozhuo Ren ◽  
Shuangshuang Ma ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Interconnected Pores ◽  
Dimensional Cell

Ho:YAG laser: intervertebral disk cell interaction using three-dimensional cell culture system

2000 ◽  
Author(s):  
Masato Sato ◽  
Miya Ishihara ◽  
Tsunenori Arai ◽  
Takashi Asazuma ◽  
Toshiyuki Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Intervertebral Disk ◽  
Cell Culture System ◽  
Ho:Yag Laser ◽  
Dimensional Cell
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