scholarly journals Cell proliferation inin vivo-like three-dimensional cell culture is regulated by sequestration of ERK1/2 to lipid rafts

2014 ◽  
Vol 47 (4) ◽  
pp. 336-346 ◽  
Author(s):  
R. Skrobanska ◽  
A. Evangelatov ◽  
N. Stefanova ◽  
T. Topouzova-Hristova ◽  
A. Momchilova ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
Lipid Rafts ◽  
Three Dimensional ◽  
Dimensional Cell

Three-dimensional cell culture of chondrocytes on modified di-phenylalanine scaffolds

2007 ◽  
Vol 35 (3) ◽  
pp. 535-537 ◽  
Author(s):  
V. Jayawarna ◽  
A. Smith ◽  
J.E. Gough ◽  
R.V. Ulijn
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Cell Proliferation ◽  
Self Assembly ◽  
Three Dimensional ◽  
Building Blocks ◽  
Culture Conditions ◽  
The Self ◽  
Three Dimensions ◽  
Dimensional Cell

The design of self-assembled peptide-based structures for three-dimensional cell culture and tissue repair has been a key objective in biomaterials science for decades. In search of the simplest possible peptide system that can self-assemble, we discovered that combinations of di-peptides that are modified with aromatic stacking ligands could form nanometre-sized fibres when exposed to physiological conditions. For example, we demonstrated that a number of Fmoc (fluoren-9-ylmethyloxycarbonyl) modified di- and tri-peptides form highly ordered hydrogels via hydrogen-bonding and π–π interactions from the fluorenyl rings. These highly hydrated gels allowed for cell proliferation of chondrocytes in three dimensions [Jayawarna, Ali, Jowitt, Miller, Saiani, Gough and Ulijn (2006) Adv. Mater. 18, 611–614]. We demonstrated that fibrous architecture and physical properties of the resulting materials were dictated by the nature of the amino acid building blocks. Here, we report the self-assembly process of three di-phenylalanine analogues, Fmoc-Phe-Phe-OH, Nap (naphthalene)-Phe-Phe-OH and Cbz (benzyloxycarbonyl)-Phe-Phe-OH, to compare and contrast the self-assembly properties and cell culture conditions attributable to their protecting group difference. Fibre morphology analysis of the three structures using cryo-SEM (scanning electron microscopy) and TEM (transmission electron microscopy) suggested fibrous structures with dramatically varying fibril dimensions, depending on the aromatic ligand used. CD and FTIR (Fourier-transform IR) data confirmed β-sheet arrangements in all three samples in the gel state. The ability of these three new hydrogels to support cell proliferation of chondrocytes was confirmed for all three materials.

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

A novel fluffy hydroxylapatite fiber scaffold with deep interconnected pores designed for three-dimensional cell culture

2014 ◽  
Vol 2 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Lin Jin ◽  
Zhang-Qi Feng ◽  
Ting Wang ◽  
Zhuozhuo Ren ◽  
Shuangshuang Ma ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Interconnected Pores ◽  
Dimensional Cell

Ho:YAG laser: intervertebral disk cell interaction using three-dimensional cell culture system

2000 ◽  
Author(s):  
Masato Sato ◽  
Miya Ishihara ◽  
Tsunenori Arai ◽  
Takashi Asazuma ◽  
Toshiyuki Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Intervertebral Disk ◽  
Cell Culture System ◽  
Ho:Yag Laser ◽  
Dimensional Cell

Preparation of Porous Organically-Modified Silicate Hybrids for Cell Culture Matrix

2007 ◽  
Vol 330-332 ◽  
pp. 1177-1180 ◽  
Author(s):  
Kanji Tsuru ◽  
Satoshi Hayakawa ◽  
Yuki Shirosaki ◽  
T. Okayama ◽  
K. Kataoka ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mammalian Cells ◽  
Pore Diameter ◽  
Sol Gel ◽  
Three Dimensional ◽  
Culture Methods ◽  
Organically Modified ◽  
Dimensional Cell

Porous & rubbery organic-inorganic hybrids were synthesized from tetraethoxysilane (TEOS) and polydimethylsiloxane (PDMS) through a sol-gel route using sieved sucrose granules as a porogen. The porous hybrids with a high content of PDMS behaved like polymer sponge. The porosity was over 90% irrespective of the hybrid composition and the pore diameter ranged from 100 to 500 μm. In the three-dimensional cell culture, mammalian cells were well cultured in the porous hybrids. The present results indicate that the hybrids may be a promising scaffold for developing such functional culture methods.

A New Technology for Three-Dimensional Cell Culture: The Hydrodynamic Focusing Bioreactor

1999 ◽  
Author(s):  
Yow-Min D. Tsao ◽  
Steve R. Gonda
Keyword(s):  
Cell Culture ◽  
New Technology ◽  
Three Dimensional ◽  
Culture Vessel ◽  
Air Bubbles ◽  
Hydrodynamic Focusing ◽  
Shaped Cell ◽  
Fluid Environment ◽  
Dimensional Cell ◽  
Low Shear

Abstract The Hydrodynamic Focusing Bioreactor (HDFB) developed by NASA at the Johnson Space Center provides a unique hydrofocusing capability that simultaneously enables a low-shear culture environment and a unique hydrofocusing-based “herding” of suspended cells, cell aggregates, and air bubbles. The HDFB is a rotating dome-shaped cell culture vessel with a centrally located sampling port and an internal rotating viscous spinner attached to a rotating base. The vessel and viscous spinner can rotate at different speeds and in either the same or different directions. Adjusting the differential rotation rate between the vessel and spinner results in a controllable hydrodynamic focusing force. The resultant hydrodynamic force suspends the cells in a low-shear fluid environment that supports the formation of delicate three-dimensional tissue assemblies. Both suspension and anchorage-dependent cells have been successfully cultured.

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