scholarly journals Hybrid magneto-luminescent iron oxide nanocubes functionalized with europium complexes: synthesis, hemolytic properties and protein corona formation

2021 ◽  
Author(s):  
Luelc Souza da Costa ◽  
Latif Ullah Khan ◽  
Lidiane Silva Franqui ◽  
Fabrício de Souza Delite ◽  
Diego Muraca ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Biomedical Applications ◽  
Protein Corona ◽  
Hybrid Nanostructures ◽  
Europium Complexes ◽  
Corona Formation

In this work, we carried out the preparation of a multifunctional hybrid nanostructures towards biomedical applications with a solid background for further applications of these materials containing new surface functionalities of nanobiotechnology.

scholarly journals Protein Corona Hinders N-CQDs Oxidative Potential and Favors Their Application as Nanobiocatalytic System

2021 ◽  
Vol 22 (15) ◽  
pp. 8136
Author(s):  
Joanna Czarnecka ◽  
Mateusz Kwiatkowski ◽  
Marek Wiśniewski ◽  
Katarzyna Roszek
Keyword(s):  
Biomedical Applications ◽  
Catalytic Efficiency ◽  
Protein Corona ◽  
Immobilized Enzymes ◽  
Oxidative Capacity ◽  
Carbon Quantum Dots ◽  
Oxidative Potential ◽  
Corona Formation ◽  
High Concentrations ◽  
Enzyme Application

The oxidative properties of nanomaterials arouse legitimate concerns about oxidative damage in biological systems. On the other hand, the undisputable benefits of nanomaterials promote them for biomedical applications; thus, the strategies to reduce oxidative potential are urgently needed. We aimed at analysis of nitrogen-containing carbon quantum dots (N-CQDs) in terms of their biocompatibility and internalization by different cells. Surprisingly, N-CQD uptake does not contribute to the increased oxidative stress inside cells and lacks cytotoxic influence even at high concentrations, primarily through protein corona formation. We proved experimentally that the protein coating effectively limits the oxidative capacity of N-CQDs. Thus, N-CQDs served as an immobilization support for three different enzymes with the potential to be used as therapeutics. Various kinetic parameters of immobilized enzymes were analyzed. Regardless of the enzyme structure and type of reaction catalyzed, adsorption on the nanocarrier resulted in increased catalytic efficiency. The enzymatic-protein-to-nanomaterial ratio is the pivotal factor determining the course of kinetic parameter changes that can be tailored for enzyme application. We conclude that the above properties of N-CQDs make them an ideal support for enzymatic drugs required for multiple biomedical applications, including personalized medical therapies.

scholarly journals Surface properties modulate protein corona formation and determine cellular uptake and cytotoxicity of silver nanoparticles

Nanoscale ◽  
2021 ◽  
Author(s):  
Marianna Barbalinardo ◽  
Jessika Bertacchini ◽  
Linda Bergamini ◽  
Maria Sara Magarò ◽  
Luca Ortolani ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Surface Properties ◽  
Cellular Uptake ◽  
Biomedical Applications ◽  
Protein Corona ◽  
Diagnosis And Treatment ◽  
Corona Formation ◽  
Basic Understanding

Nanoparticles (NPs) have been studied for biomedical applications, ranging from prevention, diagnosis and treatment of diseases. However, the lack of the basic understanding of how NPs interact with the biological...

scholarly journals Understanding the Influence of a Bifunctional Polyethylene Glycol Derivative in Protein Corona Formation around Iron Oxide Nanoparticles

Materials ◽  
2019 ◽  
Vol 12 (14) ◽  
pp. 2218 ◽  
Author(s):  
Amalia Ruiz ◽  
Adán Alpízar ◽  
Lilianne Beola ◽  
Carmen Rubio ◽  
Helena Gavilán ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Protein Corona ◽  
Raw 264.7 Cells ◽  
Oxide Nanoparticles ◽  
Mri Imaging ◽  
Corona Formation

Superparamagnetic iron oxide nanoparticles are one of the most prominent agents used in theranostic applications, with MRI imaging the main application assessed. The biomolecular interface formed on the surface of a nanoparticle in a biological medium determines its behaviour in vitro and in vivo. In this study, we have compared the formation of the protein corona on highly monodisperse iron oxide nanoparticles with two different coatings, dimercaptosuccinic acid (DMSA), and after conjugation, with a bifunctional polyethylene glycol (PEG)-derived molecule (2000 Da) in the presence of Wistar rat plasma. The protein fingerprints around the nanoparticles were analysed in an extensive proteomic study. The results presented in this work indicate that the composition of the protein corona is very difficult to predict. Proteins from different functional categories—cell components, lipoproteins, complement, coagulation, immunoglobulins, enzymes and transport proteins—were identified in all samples with very small variability. Although both types of nanoparticles have similar amounts of bonded proteins, very slight differences in the composition of the corona might explain the variation observed in the uptake and biotransformation of these nanoparticles in Caco-2 and RAW 264.7 cells. Cytotoxicity was also studied using a standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Controlling nanoparticles’ reactivity to the biological environment by deciding on its surface functionalization may suggest new routes in the control of the biodistribution, biodegradation and clearance of multifunctional nanomedicines.

scholarly journals Cover Feature: Protein Corona Formation on Magnetic Nanoparticles Conjugated with Luminescent Europium Complexes (ChemNanoMat 12/2018)

ChemNanoMat ◽  
2018 ◽  
Vol 4 (12) ◽  
pp. 1190-1190
Author(s):  
Latif U. Khan ◽  
Romana Petry ◽  
Amauri J. Paula ◽  
Marcelo Knobel ◽  
Diego Stéfani T. Martinez
Keyword(s):  
Magnetic Nanoparticles ◽  
Protein Corona ◽  
Europium Complexes ◽  
Corona Formation

Protein Corona Formation on Magnetic Nanoparticles Conjugated with Luminescent Europium Complexes

ChemNanoMat ◽  
2018 ◽  
Vol 4 (12) ◽  
pp. 1202-1208 ◽  
Author(s):  
Latif U. Khan ◽  
Romana Petry ◽  
Amauri J. Paula ◽  
Marcelo Knobel ◽  
Diego Stéfani T. Martinez
Keyword(s):  
Magnetic Nanoparticles ◽  
Protein Corona ◽  
Europium Complexes ◽  
Corona Formation

The role of morphology, shell composition and protein corona formation in Au/Fe3O4 composite nanoparticle mediated macrophage responses

2021 ◽  
Author(s):  
Lihua He ◽  
Kang Ma ◽  
Xiaonan Liu ◽  
Huixia Li ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Immune System ◽  
Physicochemical Properties ◽  
Biomedical Applications ◽  
Protein Corona ◽  
System P ◽  
Corona Formation ◽  
Composite Nanoparticle ◽  
Shell Composition

The great interest in using nanoparticles (NPs) for biomedical applications is transversal to various materials despite the poorly understood correlation between their physicochemical properties and effects on the immune system....

scholarly journals Zwitterionic Modification of Ultrasmall Iron Oxide Nanoparticles for Reduced Protein Corona Formation

ChemPlusChem ◽  
2017 ◽  
Vol 82 (4) ◽  
pp. 638-646 ◽  
Author(s):  
Karina Pombo-García ◽  
Carmen L. Rühl ◽  
Raymond Lam ◽  
José A. Barreto ◽  
Ching-Seng Ang ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Protein Corona ◽  
Oxide Nanoparticles ◽  
Corona Formation

Iron Oxide Nanoparticles: An Insight into their Biomedical Applications

2015 ◽  
Vol 22 (15) ◽  
pp. 1808-1828 ◽  
Author(s):  
Diana Couto ◽  
Marisa Freitas ◽  
Felix Carvalho ◽  
Eduarda Fernandes
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Biomedical Applications ◽  
Oxide Nanoparticles ◽  
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