scholarly journals Understanding the Influence of a Bifunctional Polyethylene Glycol Derivative in Protein Corona Formation around Iron Oxide Nanoparticles

Materials ◽  
2019 ◽  
Vol 12 (14) ◽  
pp. 2218 ◽  
Author(s):  
Amalia Ruiz ◽  
Adán Alpízar ◽  
Lilianne Beola ◽  
Carmen Rubio ◽  
Helena Gavilán ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Protein Corona ◽  
Raw 264.7 Cells ◽  
Oxide Nanoparticles ◽  
Mri Imaging ◽  
Corona Formation

Superparamagnetic iron oxide nanoparticles are one of the most prominent agents used in theranostic applications, with MRI imaging the main application assessed. The biomolecular interface formed on the surface of a nanoparticle in a biological medium determines its behaviour in vitro and in vivo. In this study, we have compared the formation of the protein corona on highly monodisperse iron oxide nanoparticles with two different coatings, dimercaptosuccinic acid (DMSA), and after conjugation, with a bifunctional polyethylene glycol (PEG)-derived molecule (2000 Da) in the presence of Wistar rat plasma. The protein fingerprints around the nanoparticles were analysed in an extensive proteomic study. The results presented in this work indicate that the composition of the protein corona is very difficult to predict. Proteins from different functional categories—cell components, lipoproteins, complement, coagulation, immunoglobulins, enzymes and transport proteins—were identified in all samples with very small variability. Although both types of nanoparticles have similar amounts of bonded proteins, very slight differences in the composition of the corona might explain the variation observed in the uptake and biotransformation of these nanoparticles in Caco-2 and RAW 264.7 cells. Cytotoxicity was also studied using a standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Controlling nanoparticles’ reactivity to the biological environment by deciding on its surface functionalization may suggest new routes in the control of the biodistribution, biodegradation and clearance of multifunctional nanomedicines.

Efficient and Rapid Labeling of Transplanted Cell Populations with Superparamagnetic Iron Oxide Nanoparticles Using Cell Surface Chemical Biotinylation for in Vivo Monitoring by MRI

2010 ◽  
Vol 19 (4) ◽  
pp. 419-429 ◽  
Author(s):  
Po-Wah So ◽  
Tammy Kalber ◽  
David Hunt ◽  
Michael Farquharson ◽  
Alia Al-Ebraheem ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cell Tracking ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Cell Populations ◽  
Oxide Nanoparticles ◽  
Signal Loss

Determination of the dynamics of specific cell populations in vivo is essential for the development of cell-based therapies. For cell tracking by magnetic resonance imaging (MRI), cells need to internalize, or be surface labeled with a MRI contrast agent, such as superparamagnetic iron oxide nanoparticles (SPIOs): SPIOs give rise to signal loss by gradient-echo and T2-weighted MRI techniques. In this study, cancer cells were chemically tagged with biotin and then magnetically labeled with anti-biotin SPIOs. No significant detrimental effects on cell viability or death were observed following cell biotinylation. SPIO-labeled cells exhibited signal loss compared to non-SPIO-labeled cells by MRI in vitro. Consistent with the in vitro MRI data, signal attenuation was observed in vivo from SPIO-labeled cells injected into the muscle of the hind legs, or implanted subcutaneously into the flanks of mice, correlating with iron detection by histochemical and X-ray fluorescence (XRF) methods. To further validate this approach, human mesenchymal stem cells (hMSCs) were also employed. Chemical biotinylation and SPIO labeling of hMSCs were confirmed by fluorescence microscopy and flow cytometry. The procedure did not affect proliferation and multipotentiality, or lead to increased cell death. The SPIO-labeled hMSCs were shown to exhibit MRI signal reduction in vitro and was detectable in an in vivo model. In this study, we demonstrate a rapid, robust, and generic methodology that may be a useful and practical adjuvant to existing methods of cell labeling for in vivo monitoring by MRI. Further, we have shown the first application of XRF to provide iron maps to validate MRI data in SPIO-labeled cell tracking studies.

Polyethylenimine-coated superparamagnetic iron oxide nanoparticles impair in vitro and in vivo angiogenesis

2019 ◽  
Vol 21 ◽  
pp. 102063 ◽  
Author(s):  
Vladimir Mulens-Arias ◽  
José Manuel Rojas ◽  
Laura Sanz-Ortega ◽  
Yadileiny Portilla ◽  
Sonia Pérez-Yagüe ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles

scholarly journals Tumor Cell Targeting by Iron Oxide Nanoparticles Is Dominated by Different Factors In Vitro versus In Vivo

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0115636 ◽  
Author(s):  
Christian NDong ◽  
Jennifer A. Tate ◽  
Warren C. Kett ◽  
Jaya Batra ◽  
Eugene Demidenko ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Tumor Cell ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Cell Targeting

scholarly journals Compact zwitterion-coated iron oxide nanoparticles for in vitro and in vivo imaging

2012 ◽  
Vol 5 (1) ◽  
pp. 108-114 ◽  
Author(s):  
He Wei ◽  
Oliver T. Bruns ◽  
Ou Chen ◽  
Moungi G. Bawendi
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
In Vivo Imaging ◽  
Oxide Nanoparticles

scholarly journals Effect of Surface Chemistry and Associated Protein Corona on the Long-Term Biodegradation of Iron Oxide Nanoparticles In Vivo

2018 ◽  
Vol 10 (5) ◽  
pp. 4548-4560 ◽  
Author(s):  
Grazyna Stepien ◽  
María Moros ◽  
Marta Pérez-Hernández ◽  
Marta Monge ◽  
Lucía Gutiérrez ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Surface Chemistry ◽  
Iron Oxide Nanoparticles ◽  
Protein Corona ◽  
Oxide Nanoparticles ◽  
Effect Of Surface

scholarly journals In Vitro/In Vivo Toxicity Evaluation and Quantification of Iron Oxide Nanoparticles

2015 ◽  
Vol 16 (10) ◽  
pp. 24417-24450 ◽  
Author(s):  
Ujwal Patil ◽  
Shiva Adireddy ◽  
Ashvin Jaiswal ◽  
Sree Mandava ◽  
Benjamin Lee ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Toxicity Evaluation ◽  
In Vivo Toxicity
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