scholarly journals Surface properties modulate protein corona formation and determine cellular uptake and cytotoxicity of silver nanoparticles

Nanoscale ◽  
2021 ◽  
Author(s):  
Marianna Barbalinardo ◽  
Jessika Bertacchini ◽  
Linda Bergamini ◽  
Maria Sara Magarò ◽  
Luca Ortolani ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Surface Properties ◽  
Cellular Uptake ◽  
Biomedical Applications ◽  
Protein Corona ◽  
Diagnosis And Treatment ◽  
Corona Formation ◽  
Basic Understanding

Nanoparticles (NPs) have been studied for biomedical applications, ranging from prevention, diagnosis and treatment of diseases. However, the lack of the basic understanding of how NPs interact with the biological...

scholarly journals Surface properties modulate protein corona formation and determine cellular uptake and cytotoxicity of silver nanoparticles

2021 ◽  
Author(s):  
Denis Gentili ◽  
Marianna Barbalinardo ◽  
Jessika Bertacchini ◽  
Linda Bergamini ◽  
Maria Sara Magarò ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Surface Properties ◽  
Cellular Uptake ◽  
Protein Corona ◽  
Corona Formation

Silver nanoparticles in complex biological media: assessment of colloidal stability and protein corona formation

2016 ◽  
Vol 18 (8) ◽  
Author(s):  
Simona Argentiere ◽  
Claudia Cella ◽  
Maura Cesaria ◽  
Paolo Milani ◽  
Cristina Lenardi
Keyword(s):  
Silver Nanoparticles ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Biological Media ◽  
Corona Formation

Protein corona formation and its influence on biomimetic magnetite nanoparticles

2020 ◽  
Vol 8 (22) ◽  
pp. 4870-4882 ◽  
Author(s):  
Ana Peigneux ◽  
Emanuel A. Glitscher ◽  
Rawan Charbaji ◽  
Christoph Weise ◽  
Stefanie Wedepohl ◽  
...  
Keyword(s):  
Human Plasma ◽  
Cellular Uptake ◽  
Magnetite Nanoparticles ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Corona Formation

Colloidal stability and cellular uptake of MamC-biomimetic magnetite nanoparticles (BMNPs) incubated with human plasma (PC-BMNPs).

Surface roughness influences the protein corona formation of glycosylated nanoparticles and alter their cellular uptake

Nanoscale ◽  
2019 ◽  
Vol 11 (48) ◽  
pp. 23259-23267 ◽  
Author(s):  
Alberto Piloni ◽  
Chin Ken Wong ◽  
Fan Chen ◽  
Megan Lord ◽  
Andreas Walther ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Cellular Uptake ◽  
Protein Corona ◽  
Biological Behavior ◽  
Protein Absorption ◽  
Corona Formation

Patterned nanoparticle surfaces can repel protein absorption and prevent the formation of a protein corona, which alters the biological behavior and therefore the fate of the nanoparticle.

scholarly journals Protein Corona Hinders N-CQDs Oxidative Potential and Favors Their Application as Nanobiocatalytic System

2021 ◽  
Vol 22 (15) ◽  
pp. 8136
Author(s):  
Joanna Czarnecka ◽  
Mateusz Kwiatkowski ◽  
Marek Wiśniewski ◽  
Katarzyna Roszek
Keyword(s):  
Biomedical Applications ◽  
Catalytic Efficiency ◽  
Protein Corona ◽  
Immobilized Enzymes ◽  
Oxidative Capacity ◽  
Carbon Quantum Dots ◽  
Oxidative Potential ◽  
Corona Formation ◽  
High Concentrations ◽  
Enzyme Application

The oxidative properties of nanomaterials arouse legitimate concerns about oxidative damage in biological systems. On the other hand, the undisputable benefits of nanomaterials promote them for biomedical applications; thus, the strategies to reduce oxidative potential are urgently needed. We aimed at analysis of nitrogen-containing carbon quantum dots (N-CQDs) in terms of their biocompatibility and internalization by different cells. Surprisingly, N-CQD uptake does not contribute to the increased oxidative stress inside cells and lacks cytotoxic influence even at high concentrations, primarily through protein corona formation. We proved experimentally that the protein coating effectively limits the oxidative capacity of N-CQDs. Thus, N-CQDs served as an immobilization support for three different enzymes with the potential to be used as therapeutics. Various kinetic parameters of immobilized enzymes were analyzed. Regardless of the enzyme structure and type of reaction catalyzed, adsorption on the nanocarrier resulted in increased catalytic efficiency. The enzymatic-protein-to-nanomaterial ratio is the pivotal factor determining the course of kinetic parameter changes that can be tailored for enzyme application. We conclude that the above properties of N-CQDs make them an ideal support for enzymatic drugs required for multiple biomedical applications, including personalized medical therapies.

scholarly journals Surface Charge-Dependent Cellular Uptake of Polystyrene Nanoparticles

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 1028 ◽  
Author(s):  
Soyeon Jeon ◽  
Jessica Clavadetscher ◽  
Dong-Keun Lee ◽  
Sunay Chankeshwara ◽  
Mark Bradley ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Surface Charge ◽  
Cellular Uptake ◽  
A549 Cells ◽  
Protein Corona ◽  
Polystyrene Nanoparticles ◽  
Biological Impact ◽  
Corona Formation ◽  
Different Types

The evaluation of the role of physicochemical properties in the toxicity of nanoparticles is important for the understanding of toxicity mechanisms and for controlling the behavior of nanoparticles. The surface charge of nanoparticles is suggested as one of the key parameters which decide their biological impact. In this study, we synthesized fluorophore-conjugated polystyrene nanoparticles (F-PLNPs), with seven different types of surface functional groups that were all based on an identical core, to evaluate the role of surface charge in the cellular uptake of nanoparticles. Phagocytic differentiated THP-1 cells or non-phagocytic A549 cells were incubated with F-PLNP for 4 h, and their cellular uptake was quantified by fluorescence intensity and confocal microscopy. The amount of internalized F-PLNPs showed a good positive correlation with the zeta potential of F-PLNPs in both cell lines (Pearson’s r = 0.7021 and 0.7852 for zeta potential vs. cellular uptake in THP-1 cells and nonphagocytic A549 cells, respectively). This result implies that surface charge is the major parameter determining cellular uptake efficiency, although other factors such as aggregation/agglomeration, protein corona formation, and compositional elements can also influence the cellular uptake partly or indirectly.

scholarly journals The Impact of Protein Corona Formation on the Macrophage Cellular Uptake and Biodistribution of Spherical Nucleic Acids

Small ◽  
2017 ◽  
Vol 13 (16) ◽  
pp. 1603847 ◽  
Author(s):  
Alyssa B. Chinen ◽  
Chenxia M. Guan ◽  
Caroline H. Ko ◽  
Chad A. Mirkin
Keyword(s):  
Nucleic Acids ◽  
Cellular Uptake ◽  
Protein Corona ◽  
Corona Formation ◽  
Spherical Nucleic Acids ◽  
The Impact

Protein Corona Formation on Colloidal Polymeric Nanoparticles and Polymeric Nanogels: Impact on Cellular Uptake, Toxicity, Immunogenicity, and Drug Release Properties

2017 ◽  
Vol 18 (6) ◽  
pp. 1762-1771 ◽  
Author(s):  
Katja Obst ◽  
Guy Yealland ◽  
Benjamin Balzus ◽  
Enrico Miceli ◽  
Mathias Dimde ◽  
...  
Keyword(s):  
Drug Release ◽  
Cellular Uptake ◽  
Polymeric Nanoparticles ◽  
Protein Corona ◽  
Corona Formation

scholarly journals Molecular Modeling of Protein Corona Formation and Its Interactions with Nanoparticles and Cell Membranes for Nanomedicine Applications

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 637
Author(s):  
Hwankyu Lee
Keyword(s):  
Surface Properties ◽  
Plasma Proteins ◽  
Cell Membranes ◽  
Competitive Adsorption ◽  
Critical Role ◽  
Protein Corona ◽  
Md Simulations ◽  
Atomic Scale ◽  
Adsorption And Desorption ◽  
Corona Formation

The conformations and surface properties of nanoparticles have been modified to improve the efficiency of drug delivery. However, when nanoparticles flow through the bloodstream, they interact with various plasma proteins, leading to the formation of protein layers on the nanoparticle surface, called protein corona. Experiments have shown that protein corona modulates nanoparticle size, shape, and surface properties and, thus, influence the aggregation of nanoparticles and their interactions with cell membranes, which can increases or decreases the delivery efficiency. To complement these experimental findings and understand atomic-level phenomena that cannot be captured by experiments, molecular dynamics (MD) simulations have been performed for the past decade. Here, we aim to review the critical role of MD simulations to understand (1) the conformation, binding site, and strength of plasma proteins that are adsorbed onto nanoparticle surfaces, (2) the competitive adsorption and desorption of plasma proteins on nanoparticle surfaces, and (3) the interactions between protein-coated nanoparticles and cell membranes. MD simulations have successfully predicted the competitive binding and conformation of protein corona and its effect on the nanoparticle–nanoparticle and nanoparticle–membrane interactions. In particular, simulations have uncovered the mechanism regarding the competitive adsorption and desorption of plasma proteins, which helps to explain the Vroman effect. Overall, these findings indicate that simulations can now provide predications in excellent agreement with experimental observations as well as atomic-scale insights into protein corona formation and interactions.

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