scholarly journals Accelerated FRET-PAINT Microscopy

2018 ◽  
Author(s):  
Jongjin Lee ◽  
Sangjun Park ◽  
Sungchul Hohng
Keyword(s):  
Fluorescence Microscopy ◽  
Super Resolution ◽  
Dissociation Rate ◽  
Dna Probes ◽  
Speed Limit ◽  
High Quality ◽  
Resolution Imaging ◽  
Bleed Through ◽  
Imaging Speed

Recent development of FRET-PAINT microscopy significantly improved the imaging speed of DNA-PAINT, the previously reported super-resolution fluorescence microscopy with no photobleaching problem. Here we try to achieve the ultimate speed limit of FRET-PAINT by optimizing the camera speed, dissociation rate of DNA probes, and bleed-through of the donor signal to the acceptor channel, and further increase the imaging speed of FRET-PAINT by 8-fold. Super-resolution imaging of COS-7 microtubules shows that high-quality 40-nm resolution images can be obtained in just tens of seconds.

Enhanced high-quality super-resolution imaging in air using microsphere lens groups

2020 ◽  
Vol 45 (11) ◽  
pp. 2981
Author(s):  
Hao Luo ◽  
Haibo Yu ◽  
Yangdong Wen ◽  
Tianyao Zhang ◽  
Pan Li ◽  
...  
Keyword(s):  
Super Resolution ◽  
High Quality ◽  
Resolution Imaging

scholarly journals Recent Progress in Light Sheet Microscopy for Biological Applications

2018 ◽  
Vol 72 (8) ◽  
pp. 1137-1169 ◽  
Author(s):  
Krishnendu Chatterjee ◽  
Feby Wijaya Pratiwi ◽  
Frances Camille M. Wu ◽  
Peilin Chen ◽  
Bi-Chang Chen
Keyword(s):  
Developmental Biology ◽  
Fluorescence Microscopy ◽  
Single Cell ◽  
Single Molecule ◽  
Super Resolution ◽  
Light Sheet ◽  
High Signal ◽  
Spatiotemporal Resolution ◽  
Light Sheet Microscopy ◽  
Resolution Imaging

The introduction of light sheet fluorescence microscopy (LSFM) has overcome the challenges in conventional optical microscopy. Among the recent breakthroughs in fluorescence microscopy, LSFM had been proven to provide a high three-dimensional spatial resolution, high signal-to-noise ratio, fast imaging acquisition rate, and minuscule levels of phototoxic and photodamage effects. The aforementioned auspicious properties are crucial in the biomedical and clinical research fields, covering a broad range of applications: from the super-resolution imaging of intracellular dynamics in a single cell to the high spatiotemporal resolution imaging of developmental dynamics in an entirely large organism. In this review, we provided a systematic outline of the historical development of LSFM, detailed discussion on the variants and improvements of LSFM, and delineation on the most recent technological advancements of LSFM and its potential applications in single molecule/particle detection, single-molecule super-resolution imaging, imaging intracellular dynamics of a single cell, multicellular imaging: cell–cell and cell–matrix interactions, plant developmental biology, and brain imaging and developmental biology.

scholarly journals Super-resolution light-sheet fluorescence microscopy by SOFI

2020 ◽  
Author(s):  
Judith Mizrachi ◽  
Arun Narasimhan ◽  
Xiaoli Qi ◽  
Rhonda Drewes ◽  
Ramesh Palaniswamy ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Ampa Receptors ◽  
Super Resolution ◽  
Light Sheet ◽  
Cytoskeletal Proteins ◽  
The Body ◽  
Synaptic Proteins ◽  
Resolution Analysis ◽  
Resolution Imaging ◽  
Light Sheet Fluorescence Microscopy

Here we describe a new method, named LS-SOFI, that combines light-sheet fluorescence microscopy and super-resolution optical fluctuation imaging to achieve fast nanoscale-resolution imaging over large fields of view in native 3D tissues. We demonstrate the use of LS-SOFI in super-resolution analysis of neuronal structures and synaptic proteins, including cortical axons, dendritic spines, pre- and postsynaptic cytoskeletal proteins and postsynaptic AMPA receptors, in thick mouse brain sections. We also introduce an algorithm to determine the number of active fluorophore emitters detected, allowing the localization of individual molecules in LS-SOFI images. We conclude that LS-SOFI is a versatile method for fast super-resolution imaging from any tissue of the body using both commercial and custom LSFM instruments.

Super-resolution imaging of STAT3 cellular clustering during nuclear transport

RSC Advances ◽  
2016 ◽  
Vol 6 (59) ◽  
pp. 54597-54607 ◽  
Author(s):  
Jing Gao ◽  
Feng Wang ◽  
Junling Chen ◽  
Jianzhong Wang ◽  
Mingjun Cai ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Nuclear Transport ◽  
Super Resolution ◽  
Resolution Imaging

STAT3 cellular clustering revealed by super-resolution fluorescence microscopy.

scholarly journals N-Aryl Pyrido Cyanine derivatives: nuclear and organelle DNA markers for two-photon and super-resolution imaging

2020 ◽  
Author(s):  
Kakishi Uno ◽  
Nagisa Sugimoto ◽  
Yoshikatsu Sato
Keyword(s):  
Nuclear Dna ◽  
Uv Light ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Dna Probes ◽  
Stimulated Emission ◽  
Cell Permeability ◽  
Organelle Dna ◽  
Two Photon ◽  
Resolution Imaging

AbstractLive cell imaging using DNA-binding fluorescent probes is an essential molecular tool in various biological and biomedical fields. The major challenges in currently used DNA probes are to avoid UV light photo-excitation with high DNA selectivity and cell-permeability and are the availability of the cutting-edge imaging techniques such as a super-resolution microscopy. Herein we report new orange to red fluorogenic DNA probes having N-aryl pyrido cyanine (PC) moiety as a basic skeleton. Their DNA selectivity and cell-permeabilities are so high that organelle DNA as well as nuclear DNA can be clearly stained in various cell types and plant tissues with wash-free manner. PC dyes are also compatible with a stimulated emission depletion fluorescent lifetime imaging microscopy (STED-FLIM) for super-resolution imaging as well as two-photon microscopy for deep tissue imaging, should release the utilization limitation of synthetic DNA probes.

A polymer index-matched to water enables diverse applications in fluorescence microscopy

Lab on a Chip ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 1549-1562
Author(s):  
Xiaofei Han ◽  
Yijun Su ◽  
Hamilton White ◽  
Kate M. O'Neill ◽  
Nicole Y. Morgan ◽  
...  
Keyword(s):  
Refractive Index ◽  
Fluorescence Microscopy ◽  
Biological Samples ◽  
Super Resolution ◽  
Resolution Imaging

Diffraction-limited and super-resolution imaging of biological samples using refractive-index matched polymers in microdevices.

Performance and applications of a field-emission gun TEM/STEM

1992 ◽  
Vol 50 (2) ◽  
pp. 942-943
Author(s):  
Judith M. Brock ◽  
Max T. Otten ◽  
Marc. J.C. de Jong
Keyword(s):  
Field Emission ◽  
High Resolution Imaging ◽  
High Quality ◽  
Small Energy ◽  
High Brightness ◽  
Small Probe ◽  
Resolution Imaging ◽  
Convergent Beam ◽  
Beam Patterns ◽  
Beam Diffraction

A Field Emission Gun (FEG) on a TEM/STEM instrument provides a major improvement in performance relative to one equipped with a LaB6 emitter. The improvement is particularly notable for small-probe techniques: EDX and EELS microanalysis, convergent beam diffraction and scanning. The high brightness of the FEG (108 to 109 A/cm2srad), compared with that of LaB6 (∼106), makes it possible to achieve high probe currents (∼1 nA) in probes of about 1 nm, whilst the currents for similar probes with LaB6 are about 100 to 500x lower. Accordingly the small, high-intensity FEG probes make it possible, e.g., to analyse precipitates and monolayer amounts of segregation on grain boundaries in metals or ceramics (Fig. 1); obtain high-quality convergent beam patterns from heavily dislocated materials; reliably detect 1 nm immuno-gold labels in biological specimens; and perform EDX mapping at nm-scale resolution even in difficult specimens like biological tissue.The high brightness and small energy spread of the FEG also bring an advantage in high-resolution imaging by significantly improving both spatial and temporal coherence.

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