scholarly journals Development of targeted STORM for super resolution imaging of biological samples using digital micro-mirror device

2017 ◽  
Vol 404 ◽  
pp. 18-22 ◽  
Author(s):  
Liyana Valiya Peedikakkal ◽  
Victoria Steventon ◽  
Andrew Furley ◽  
Ashley J. Cadby
Keyword(s):  
Biological Samples ◽  
Super Resolution ◽  
Resolution Imaging

scholarly journals Three-Dimensional Super-resolution Imaging of Thick Biological Samples

2009 ◽  
Vol 15 (S2) ◽  
pp. 36-37
Author(s):  
A Vaziri ◽  
J Tang ◽  
H Shroff ◽  
C Shank
Keyword(s):  
Biological Samples ◽  
Three Dimensional ◽  
Super Resolution ◽  
Resolution Imaging

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009

scholarly journals Label-free super-resolution imaging below 90-nm using photon-reassignment

2021 ◽  
Vol 1 ◽  
pp. 3
Author(s):  
Alberto Aguilar ◽  
Adeline Boyreau ◽  
Pierre Bon
Keyword(s):  
Biological Samples ◽  
Optical Resolution ◽  
Super Resolution ◽  
Cost Effective ◽  
Label Free ◽  
Cost Effective Approach ◽  
Imaging Mode ◽  
Resolution Imaging ◽  
Fluorescent Tags ◽  
Super Resolution Microscopy

Background: Achieving resolutions below 100 nm is key for many fields, including biology and nanomaterial characterization. Although nearfield and electron microscopy are the gold standards for studying the nanoscale, optical microscopy has seen its resolution drastically improve in the last decades. So-called super-resolution microscopy is generally based on fluorescence photophysics and requires modification of the sample at least by adding fluorescent tags, an inevitably invasive step. Therefore, it remains very challenging and rewarding to achieve optical resolutions beyond the diffraction limit in label-free samples. Methods: Here, we present a breakthrough to unlock label-free 3D super-resolution imaging of any object including living biological samples. It is based on optical photon-reassignment in confocal reflectance imaging mode. Results: We demonstrate that we surpass the resolution of all fluorescence-based confocal systems by a factor ~1.5. We have obtained images with a 3D (x,y,z) optical resolution of (86x86x248) nm3 using a visible wavelength (445 nm) and a regular microscope objective (NA=1.3). The results are presented on nanoparticles as well as on (living) biological samples. Conclusions: This cost-effective approach double the resolution of reflectance confocal microscope with minimal modifications. It is therefore compatible with any microscope and sample, works in real-time, and does not require any signal processing.

scholarly journals Multilayer Three-dimensional Super-resolution Imaging of Thick Biological Samples

2009 ◽  
Vol 96 (3) ◽  
pp. 17a
Author(s):  
Alipasha Vaziri ◽  
Jianyong Tang ◽  
Hari Shroff ◽  
Charles Shank
Keyword(s):  
Biological Samples ◽  
Three Dimensional ◽  
Super Resolution ◽  
Resolution Imaging

Live-cell 3D super-resolution imaging in thick biological samples

2011 ◽  
Vol 8 (12) ◽  
pp. 1047-1049 ◽  
Author(s):  
Francesca Cella Zanacchi ◽  
Zeno Lavagnino ◽  
Michela Perrone Donnorso ◽  
Alessio Del Bue ◽  
Laura Furia ◽  
...  
Keyword(s):  
Biological Samples ◽  
Super Resolution ◽  
Live Cell ◽  
Resolution Imaging

A polymer index-matched to water enables diverse applications in fluorescence microscopy

Lab on a Chip ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 1549-1562
Author(s):  
Xiaofei Han ◽  
Yijun Su ◽  
Hamilton White ◽  
Kate M. O'Neill ◽  
Nicole Y. Morgan ◽  
...  
Keyword(s):  
Refractive Index ◽  
Fluorescence Microscopy ◽  
Biological Samples ◽  
Super Resolution ◽  
Resolution Imaging

Diffraction-limited and super-resolution imaging of biological samples using refractive-index matched polymers in microdevices.

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