Rapid sub-micromolar amperometric enzyme biosensing with free substrate access but without nanomaterial signalling support: oxidase-based glucose detection as a proof-of-principle example

2020 ◽  
Vol 56 (52) ◽  
pp. 7132-7135
Author(s):  
Kittiya Sakdaphetsiri ◽  
Thana Thaweeskulchai ◽  
Albert Schulte
Keyword(s):  
Glucose Oxidase ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Glucose Detection ◽  
Gelatin Films ◽  
Pt Electrodes ◽  
Vapor Treatment ◽  
Proof Of Principle

Reported is a high-sensitivity glucose biosensor with a simple since nanomaterial-free layout of the signalling platform. Drop-dried glucose oxidase was covalently fixed to drop-dried gelatin films on Pt-electrodes, by glutaraldehyde vapor treatment.

Glucose Biosensor Based on Glucose Oxidase-Horseradish Peroxidase/Multiporous Tin Oxide (SnO2) Modified Electrode

2021 ◽  
Vol 21 (5) ◽  
pp. 3059-3064
Author(s):  
A. K. M. Kafi ◽  
Aizam Bin Kasri ◽  
Rajan Jose
Keyword(s):  
Horseradish Peroxidase ◽  
Glucose Oxidase ◽  
Tin Oxide ◽  
Electrode Potential ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Glucose Detection ◽  
Lower Detection Limit ◽  
Vis Spectroscopy ◽  
Lower Detection

The fabrication of a network of glucose oxidase-horseradish peroxidase/tin oxide (GOx-HRP/SnO2), immobilized onto a glassy carbon electrode (GCE) and its utilization as a biosensor for glucose detection is reported. The network established with GOx-HRP/SnO2 possess high sensitivity and stability by performing the electrocatalytic features in the sensing of glucose. The turbidity of fabrication had been scanned and analyzed using UV-vis spectroscopy. The morphology and composition of the fabricated GOx-HRP/SnO2 networks were characterized by scanning electron microscopy (SEM). Cyclic voltammetry and amperometry were employed to study the electrochemical properties of the proposed biosensor. The effect of applied electrode potential and pH were systemically investigated. The biosensor responds to glucose at work potential values between −400 mV, and exhibited a lower detection limit (0.025 mM) and long linear range (0.25 to 7.0 mM), and was resistant to common interferences.

A new electrochemiluminescence biosensor for the detection of glucose based on polypyrrole/polyluminol/Ni(OH)2–C3N4/glucose oxidase-modified graphite electrode

2018 ◽  
Vol 10 (47) ◽  
pp. 5723-5730 ◽  
Author(s):  
Maryam Hamtak ◽  
Morteza Hosseini ◽  
Lida Fotouhi ◽  
Mustafa Aghazadeh
Keyword(s):  
Glucose Oxidase ◽  
Acidic Medium ◽  
Graphite Electrode ◽  
Glucose Biosensor ◽  
Glucose Detection

A new electrochemiluminescence (ECL) glucose biosensor based on polypyrrole/polyluminol/C3N4–Ni(OH)2/glucose oxidase (Ppy/Plu/C3N4–Ni(OH)2/GOx) was fabricated by the electropolymerization of pyrrole and luminol in acidic medium for glucose detection.

scholarly journals Novel amperometric glucose biosensor based on MXene nanocomposite

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
R. B. Rakhi ◽  
Pranati Nayak ◽  
Chuan Xia ◽  
Husam N. Alshareef
Keyword(s):  
Au Nanoparticles ◽  
Synergistic Effects ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Glucose Detection ◽  
Carbon Electrode ◽  
Potential Candidate ◽  
Amperometric Response ◽  
Excellent Stability ◽  
Electrochemical Transducer

Abstract A biosensor platform based on Au/MXene nanocomposite for sensitive enzymatic glucose detection is reported. The biosensor leverages the unique electrocatalytic properties and synergistic effects between Au nanoparticles and MXene sheets. An amperometric glucose biosensor is fabricated by the immobilization of glucose oxidase (GOx) enzyme on Nafion solubilized Au/ MXene nanocomposite over glassy carbon electrode (GCE). The biomediated Au nanoparticles play a significant role in facilitating the electron exchange between the electroactive center of GOx and the electrode. The GOx/Au/MXene/Nafion/GCE biosensor electrode displayed a linear amperometric response in the glucose concentration range from 0.1 to 18 mM with a relatively high sensitivity of 4.2 μAmM−1 cm−2 and a detection limit of 5.9 μM (S/N = 3). Furthermore, the biosensor exhibited excellent stability, reproducibility and repeatability. Therefore, the Au/MXene nanocomposite reported in this work is a potential candidate as an electrochemical transducer in electrochemical biosensors.

scholarly journals Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Sabriye Yusan ◽  
Mokhlesur M. Rahman ◽  
Nasir Mohamad ◽  
Tengku M. Arrif ◽  
Ahmad Zubaidi A. Latif ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Ph Value ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Calibration Plot ◽  
Carbon Paste ◽  
Linear Calibration ◽  
Effects Of Ph ◽  
Paste Electrode ◽  
Mcm 41

A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibration plot is obtained over a wide concentration range of glucose from 1 × 10−5 to 2 × 10−3 M. Under optimal conditions, the biosensor exhibits high sensitivity (0.34 µA·mM−1), low detection limit (1 × 10−4 M), high affinity to glucose (Km = 0.02 mM), and also good reproducibility (R.S.D. 2.8%, n=10) and a stability of about ten days when stored dry at +4°C. Besides, the effects of pH value, scan rate, mediator effects on the glucose current, and electroactive interference of the biosensor were also discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility.

A Novel and Simple Electrochemical Glucose Biosensor Based on a Silicon Nanowire Array Electrode

2014 ◽  
Author(s):  
Che-Wei Hsu ◽  
Wen-Chao Feng ◽  
Kang J. Chang ◽  
Gou-Jen Wang
Keyword(s):  
Low Cost ◽  
Silicon Nanowire ◽  
Nanowire Array ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Glucose Detection ◽  
Redox Peak ◽  
Array Electrode ◽  
Silicon Nanowire Array ◽  
Very High

In this study, a novel and simple electrochemical glucose biosensor based on a silicon nanowire array (SNA) electrode was proposed. Metal-assisted etching (MAE) method using an AgNO3 and HF mixing solution as the etchant was employed to grow the silicon nanowire array (SNA) electrode. A thin gold shell is then sputtered over each silicon nanowire. Potassium ferricyanide, glucose oxidase (GOx), and a Nafion thin film were then sequentially coated onto the fabricated SNA for glucose detection. The processing time of the MAE and sputtering as well as the GOx concentration were optimized in terms of the redox peak currents of the SNA electrode. Compared with the corresponding plane gold electrode, the effective sensing area of the synthesized SNA electrode was measured to be 6.12 folds. Actual glucose detections demonstrated that the proposed SNA array electrode could operate in a linear range of 0.55 mM-11.02 mM and a very high sensitivity of 346 μA mM−1 cm−2. The proposed SNA electrode based glucose biosensor possesses advantages of simple fabrication process, low cost, and high sensitivity. It is feasible for future clinical applications.

scholarly journals Review on Immobilization of Nanoparticles for Fabrication of Glucose Biosensor

2015 ◽  
Vol 773-774 ◽  
pp. 720-724
Author(s):  
N.F.S. Anuar ◽  
Halina Misran ◽  
Abreeza Manap ◽  
S.Z. Othman
Keyword(s):  
Glucose Oxidase ◽  
Enzyme Immobilization ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Glucose Measurement ◽  
Diabetic Patients ◽  
Specific Substrate ◽  
Metabolic Reaction ◽  
Response Behavior ◽  
Biosensor Application

Glucose biosensor has been improvised from time to time in order to provide fast and accurate detection of glucose concentration especially for diabetic patients. Recently, nanoparticles have rising attention engaging with biosensor application. Nanoparticles serve as the carrier of enzyme immobilization whereas; enzyme is a biological catalyst that reacts with specific substrate in metabolic reaction. Glucose oxidase (GOx) in particular, is the most common enzyme used for fabrication of biosensor. Glucose measurement was done by using amperometric measurement that converted corresponding biochemical reaction between glucose and GOx into electrical output. Response behavior studies were conducted in order to compare the successfulness of GOx immobilization onto GOx-biosensor. Immobilization of glucose oxidase onto nanoparticles can lead towards tremendous impacts especially in new, high sensitivity biosensor.

scholarly journals A glucose biosensor based on the immobilization of glucose oxidase and Au nanocomposites with polynorepinephrine

RSC Advances ◽  
2019 ◽  
Vol 9 (29) ◽  
pp. 16439-16446 ◽  
Author(s):  
Yang Liu ◽  
Xu Nan ◽  
Wei Shi ◽  
Xin Liu ◽  
Zi He ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
High Sensitivity ◽  
Fast Response ◽  
Glucose Biosensor ◽  
Au Electrode ◽  
Menten Constant

The PNE/GOD/AuNPs@PNE/Au electrode exhibited a low Michaelis–Menten constant, a fast response to glucose, outstanding anti-interference ability and high sensitivity.

DNA as a Support for Glucose Oxidase Immobilization at Prussian Blue-Modified Glassy Carbon Electrode in Biosensor Preparation

2006 ◽  
Vol 6 (11) ◽  
pp. 3539-3542
Author(s):  
A. K. M. Kafi ◽  
Dong-Yun Lee ◽  
Sang-Hyun Park ◽  
Young-Soo Kwon
Keyword(s):  
Prussian Blue ◽  
Glucose Oxidase ◽  
Glassy Carbon ◽  
Glucose Concentration ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Experimental Conditions ◽  
Sensor Performance ◽  
Relative Standard ◽  
Sensitivity And Selectivity

An amperometric glucose biosensor has been developed using DNA as a matrix of Glucose oxidase (GOx) at Prussian-blue (PB)-modified glassy carbon (GC) electrode. GC electrode was chemically modified by the PB. GOx was immobilized together with DNA at the working area of the PB-modified electrode by placing a drop of the mixture of DNA and GOx. The response of the biosensor for glucose was evaluated amperometrically. Upon immobilization of glucose oxidase with DNA, the biosensor showed rapid response toward the glucose. On the other hand, no significant response was obtained in the absence of DNA. Experimental conditions influencing the biosensor performance were optimized and assessed. This biosensor offered an excellent electrochemical response for glucose concentration in μ mol level with high sensitivity and selectivity and short response time. The levels of the relative standard deviation (RSDs), (<4%) for the entire analyses reflected a highly reproducible sensor performance. Through the use of optimized conditions, a linear relationship between current and glucose concentration was obtained up to 4 × 10−4 M. In addition, this biosensor showed high reproducibility and stability.

scholarly journals Microelectrode glucose biosensor based on nanoporous platinum/graphene oxide nanostructure for rapid glucose detection of tomato and cucumber fruits

2022 ◽  
Author(s):  
Binfang Wu ◽  
Haitao Xu ◽  
Yufeng Shi ◽  
Zhijie Yao ◽  
Jiayu Yu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Three Dimensional ◽  
Glucose Biosensor ◽  
Detection Sensitivity ◽  
Nanoporous Structure ◽  
Glucose Detection ◽  
Glucose Content ◽  
Wide Range ◽  
Nanoporous Platinum

Abstract Microelectrode glucose biosensor based on three-dimensional hybrid nanoporous platinum/graphene oxide nanostructure was developed for rapid glucose detection of tomato and cucumber fruits. The nanostructure was fabricated by a two-step modification method on microelectrode for loading a larger amount of glucose oxidase. The nanoporous structure was prepared on the surface of the platinum microelectrode by electrochemical etching, and then graphene oxide was deposited on the prepared nanoporous electrode by electrochemical deposition. The nanoprorous platinum/graphene oxide nanostructure had the advantage of improving the effective surface area of the electrode and the loading quantity of glucose oxidase. As a result, the biosensor achieved a wide range of 0.1-20.0 mM in glucose detection, which had the ability to accurately detect the glucose content. It was found that the three-dimensional hybrid nanostructure on the electrode surface realized the rapid direct electrochemistry of glucose oxidase. Therefore, the biosensor achieved high glucose detection sensitivity (11.64 μA mM -1cm -2), low detection limit (13 μM) and rapid response time (reaching 95% steady-state response within 3 seconds), when calibrating in glucose standard solution. In agricultural application, the as-prepared biosensor was employed to detect the glucose concentration of tomato and cucumber samples. The results showed that the relative deviation of this method was less than 5% when compared with that of HPLC, implying high accuracy of the presented biosensor in glucose detection in plants.

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