scholarly journals A glucose biosensor based on the immobilization of glucose oxidase and Au nanocomposites with polynorepinephrine

RSC Advances ◽  
2019 ◽  
Vol 9 (29) ◽  
pp. 16439-16446 ◽  
Author(s):  
Yang Liu ◽  
Xu Nan ◽  
Wei Shi ◽  
Xin Liu ◽  
Zi He ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
High Sensitivity ◽  
Fast Response ◽  
Glucose Biosensor ◽  
Au Electrode ◽  
Menten Constant

The PNE/GOD/AuNPs@PNE/Au electrode exhibited a low Michaelis–Menten constant, a fast response to glucose, outstanding anti-interference ability and high sensitivity.

Glucose Biosensor Based on Glucose Oxidase-Horseradish Peroxidase/Multiporous Tin Oxide (SnO2) Modified Electrode

2021 ◽  
Vol 21 (5) ◽  
pp. 3059-3064
Author(s):  
A. K. M. Kafi ◽  
Aizam Bin Kasri ◽  
Rajan Jose
Keyword(s):  
Horseradish Peroxidase ◽  
Glucose Oxidase ◽  
Tin Oxide ◽  
Electrode Potential ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Glucose Detection ◽  
Lower Detection Limit ◽  
Vis Spectroscopy ◽  
Lower Detection

The fabrication of a network of glucose oxidase-horseradish peroxidase/tin oxide (GOx-HRP/SnO2), immobilized onto a glassy carbon electrode (GCE) and its utilization as a biosensor for glucose detection is reported. The network established with GOx-HRP/SnO2 possess high sensitivity and stability by performing the electrocatalytic features in the sensing of glucose. The turbidity of fabrication had been scanned and analyzed using UV-vis spectroscopy. The morphology and composition of the fabricated GOx-HRP/SnO2 networks were characterized by scanning electron microscopy (SEM). Cyclic voltammetry and amperometry were employed to study the electrochemical properties of the proposed biosensor. The effect of applied electrode potential and pH were systemically investigated. The biosensor responds to glucose at work potential values between −400 mV, and exhibited a lower detection limit (0.025 mM) and long linear range (0.25 to 7.0 mM), and was resistant to common interferences.

Chitosan coated on the layers’ glucose oxidase immobilized on cysteamine/Au electrode for use as glucose biosensor

2014 ◽  
Vol 60 ◽  
pp. 271-276 ◽  
Author(s):  
Yawen Zhang ◽  
Yunqiu Li ◽  
Wenjian Wu ◽  
Yuren Jiang ◽  
Biru Hu
Keyword(s):  
Glucose Oxidase ◽  
Glucose Biosensor ◽  
Au Electrode

scholarly journals Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Sabriye Yusan ◽  
Mokhlesur M. Rahman ◽  
Nasir Mohamad ◽  
Tengku M. Arrif ◽  
Ahmad Zubaidi A. Latif ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Ph Value ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Calibration Plot ◽  
Carbon Paste ◽  
Linear Calibration ◽  
Effects Of Ph ◽  
Paste Electrode ◽  
Mcm 41

A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibration plot is obtained over a wide concentration range of glucose from 1 × 10−5 to 2 × 10−3 M. Under optimal conditions, the biosensor exhibits high sensitivity (0.34 µA·mM−1), low detection limit (1 × 10−4 M), high affinity to glucose (Km = 0.02 mM), and also good reproducibility (R.S.D. 2.8%, n=10) and a stability of about ten days when stored dry at +4°C. Besides, the effects of pH value, scan rate, mediator effects on the glucose current, and electroactive interference of the biosensor were also discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility.

scholarly journals Review on Immobilization of Nanoparticles for Fabrication of Glucose Biosensor

2015 ◽  
Vol 773-774 ◽  
pp. 720-724
Author(s):  
N.F.S. Anuar ◽  
Halina Misran ◽  
Abreeza Manap ◽  
S.Z. Othman
Keyword(s):  
Glucose Oxidase ◽  
Enzyme Immobilization ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Glucose Measurement ◽  
Diabetic Patients ◽  
Specific Substrate ◽  
Metabolic Reaction ◽  
Response Behavior ◽  
Biosensor Application

Glucose biosensor has been improvised from time to time in order to provide fast and accurate detection of glucose concentration especially for diabetic patients. Recently, nanoparticles have rising attention engaging with biosensor application. Nanoparticles serve as the carrier of enzyme immobilization whereas; enzyme is a biological catalyst that reacts with specific substrate in metabolic reaction. Glucose oxidase (GOx) in particular, is the most common enzyme used for fabrication of biosensor. Glucose measurement was done by using amperometric measurement that converted corresponding biochemical reaction between glucose and GOx into electrical output. Response behavior studies were conducted in order to compare the successfulness of GOx immobilization onto GOx-biosensor. Immobilization of glucose oxidase onto nanoparticles can lead towards tremendous impacts especially in new, high sensitivity biosensor.

scholarly journals Enhancement of amperometric response of glucose biosensor by electrodeposition of silver nanoparticles onto chitosan-modified electrode

2017 ◽  
Author(s):  
Hossein Zare ◽  
Ghasem Najafpour ◽  
Mohsen Jahanshahi ◽  
Mostafa Rahimnejad ◽  
Mohsen Rezvani
Keyword(s):  
Silver Nanoparticles ◽  
Modified Electrode ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Single Pulse ◽  
Glucose Biosensor ◽  
Amperometric Response ◽  
Highly Sensitive ◽  
Menten Constant ◽  
Electrodeposition Of Silver

A highly sensitive biosensor based on silver nanoparticles (AgNPs) was fabricated for glucose detection in aqueous phase. Firstly, a platinum (Pt) electrode was modified with the mixture of glucose oxidase and chitosan. AgNPs were electrodeposited into the modified electrode by single pulse potentiostatic method at –0.4 V. The electrochemical performance of the modified electrode was evaluated by cyclic voltammetry and amperometry. The fabricated biosensor had a high sensitivity of 58.6 μA mM−1 cm−2 and detection limit of 4.4 μM glucose at a signal to noise ratio of 3. In addition, the biosensor showed a short response time less than 5 s and a wide linear range of 0.05-11.5 mM. The apparent Michaelis–Menten constant (KM) was found to be 9.14 mM. In addition, thermal stability and anti-interference ability of the biosensor were investigated. The results demonstrated that AgNPs enhanced the analytical performance of the biosensor.

Rapid sub-micromolar amperometric enzyme biosensing with free substrate access but without nanomaterial signalling support: oxidase-based glucose detection as a proof-of-principle example

2020 ◽  
Vol 56 (52) ◽  
pp. 7132-7135
Author(s):  
Kittiya Sakdaphetsiri ◽  
Thana Thaweeskulchai ◽  
Albert Schulte
Keyword(s):  
Glucose Oxidase ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Glucose Detection ◽  
Gelatin Films ◽  
Pt Electrodes ◽  
Vapor Treatment ◽  
Proof Of Principle

Reported is a high-sensitivity glucose biosensor with a simple since nanomaterial-free layout of the signalling platform. Drop-dried glucose oxidase was covalently fixed to drop-dried gelatin films on Pt-electrodes, by glutaraldehyde vapor treatment.

Highly Sensitive Enzyme-Less Glucose Biosensor Based on α-Fe2O3 Nanoparticles

2018 ◽  
Vol 10 (3) ◽  
pp. 429-434 ◽  
Author(s):  
Ahmad Umar ◽  
Kulvinder Singh ◽  
S. K. Mehta ◽  
H. Fouad ◽  
Othman Y. Alothman
Keyword(s):  
Glucose Sensor ◽  
Hydrothermal Process ◽  
High Sensitivity ◽  
Fast Response ◽  
Glucose Biosensor ◽  
High Reproducibility ◽  
Highly Sensitive ◽  
Rhombohedral Crystal ◽  
Coefficient Of Regression ◽  
Facile Hydrothermal Process

This paper reports the synthesis, characterization and fabrication of enzyme-less glucose sensor based on iron oxide (α-Fe2O3) nanoparticles synthesized by facile hydrothermal process. The synthesized nanoparticles were characterized by various techniques to understand the morphologies, composition and scattering properties. The morphological and structural studies confirmed the successful formation of α-Fe2O3 nanoparticles in large quantity which exhibiting well-crystallinity and rhombohedral crystal structures. The fabricated enzyme-less glucose sensor based on α-Fe2O3 nanoparticles revealed high sensitivity of 30.89 μA/mM cm2 with a fast response time (5 sec). The fabricated glucose sensor exhibited high reproducibility and its electrochemical response found to be linear over a wide concentration range of glucose from 1 to 10 mM with coefficient of regression (R) = 0.9981.

DNA as a Support for Glucose Oxidase Immobilization at Prussian Blue-Modified Glassy Carbon Electrode in Biosensor Preparation

2006 ◽  
Vol 6 (11) ◽  
pp. 3539-3542
Author(s):  
A. K. M. Kafi ◽  
Dong-Yun Lee ◽  
Sang-Hyun Park ◽  
Young-Soo Kwon
Keyword(s):  
Prussian Blue ◽  
Glucose Oxidase ◽  
Glassy Carbon ◽  
Glucose Concentration ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Experimental Conditions ◽  
Sensor Performance ◽  
Relative Standard ◽  
Sensitivity And Selectivity

An amperometric glucose biosensor has been developed using DNA as a matrix of Glucose oxidase (GOx) at Prussian-blue (PB)-modified glassy carbon (GC) electrode. GC electrode was chemically modified by the PB. GOx was immobilized together with DNA at the working area of the PB-modified electrode by placing a drop of the mixture of DNA and GOx. The response of the biosensor for glucose was evaluated amperometrically. Upon immobilization of glucose oxidase with DNA, the biosensor showed rapid response toward the glucose. On the other hand, no significant response was obtained in the absence of DNA. Experimental conditions influencing the biosensor performance were optimized and assessed. This biosensor offered an excellent electrochemical response for glucose concentration in μ mol level with high sensitivity and selectivity and short response time. The levels of the relative standard deviation (RSDs), (<4%) for the entire analyses reflected a highly reproducible sensor performance. Through the use of optimized conditions, a linear relationship between current and glucose concentration was obtained up to 4 × 10−4 M. In addition, this biosensor showed high reproducibility and stability.

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