A new electrochemiluminescence biosensor for the detection of glucose based on polypyrrole/polyluminol/Ni(OH)2–C3N4/glucose oxidase-modified graphite electrode

2018 ◽  
Vol 10 (47) ◽  
pp. 5723-5730 ◽  
Author(s):  
Maryam Hamtak ◽  
Morteza Hosseini ◽  
Lida Fotouhi ◽  
Mustafa Aghazadeh
Keyword(s):  
Glucose Oxidase ◽  
Acidic Medium ◽  
Graphite Electrode ◽  
Glucose Biosensor ◽  
Glucose Detection

A new electrochemiluminescence (ECL) glucose biosensor based on polypyrrole/polyluminol/C3N4–Ni(OH)2/glucose oxidase (Ppy/Plu/C3N4–Ni(OH)2/GOx) was fabricated by the electropolymerization of pyrrole and luminol in acidic medium for glucose detection.

Glucose Biosensor Based on Glucose Oxidase-Horseradish Peroxidase/Multiporous Tin Oxide (SnO2) Modified Electrode

2021 ◽  
Vol 21 (5) ◽  
pp. 3059-3064
Author(s):  
A. K. M. Kafi ◽  
Aizam Bin Kasri ◽  
Rajan Jose
Keyword(s):  
Horseradish Peroxidase ◽  
Glucose Oxidase ◽  
Tin Oxide ◽  
Electrode Potential ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Glucose Detection ◽  
Lower Detection Limit ◽  
Vis Spectroscopy ◽  
Lower Detection

The fabrication of a network of glucose oxidase-horseradish peroxidase/tin oxide (GOx-HRP/SnO2), immobilized onto a glassy carbon electrode (GCE) and its utilization as a biosensor for glucose detection is reported. The network established with GOx-HRP/SnO2 possess high sensitivity and stability by performing the electrocatalytic features in the sensing of glucose. The turbidity of fabrication had been scanned and analyzed using UV-vis spectroscopy. The morphology and composition of the fabricated GOx-HRP/SnO2 networks were characterized by scanning electron microscopy (SEM). Cyclic voltammetry and amperometry were employed to study the electrochemical properties of the proposed biosensor. The effect of applied electrode potential and pH were systemically investigated. The biosensor responds to glucose at work potential values between −400 mV, and exhibited a lower detection limit (0.025 mM) and long linear range (0.25 to 7.0 mM), and was resistant to common interferences.

Fabrication of a novel disposable glucose biosensor using an electrochemically reduced graphene oxide–glucose oxidase biocomposite

2016 ◽  
Vol 8 (38) ◽  
pp. 6974-6981 ◽  
Author(s):  
Kathiresan Vijayaraj ◽  
Suck Won Hong ◽  
Sung-Ho Jin ◽  
Seung-Cheol Chang ◽  
Deog-Su Park
Keyword(s):  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Electrochemical Method ◽  
Graphite Electrode ◽  
Cost Effective ◽  
Glucose Biosensor ◽  
Pencil Graphite Electrode ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

A disposable glucose biosensor has been fabricated on the surface of a cost-effective pencil graphite electrode (PGE) by an electrochemical method, using glucose oxidase (GOx) and reduced graphene oxide (rGO).

Rapid sub-micromolar amperometric enzyme biosensing with free substrate access but without nanomaterial signalling support: oxidase-based glucose detection as a proof-of-principle example

2020 ◽  
Vol 56 (52) ◽  
pp. 7132-7135
Author(s):  
Kittiya Sakdaphetsiri ◽  
Thana Thaweeskulchai ◽  
Albert Schulte
Keyword(s):  
Glucose Oxidase ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Glucose Detection ◽  
Gelatin Films ◽  
Pt Electrodes ◽  
Vapor Treatment ◽  
Proof Of Principle

Reported is a high-sensitivity glucose biosensor with a simple since nanomaterial-free layout of the signalling platform. Drop-dried glucose oxidase was covalently fixed to drop-dried gelatin films on Pt-electrodes, by glutaraldehyde vapor treatment.

scholarly journals Microelectrode glucose biosensor based on nanoporous platinum/graphene oxide nanostructure for rapid glucose detection of tomato and cucumber fruits

2022 ◽  
Author(s):  
Binfang Wu ◽  
Haitao Xu ◽  
Yufeng Shi ◽  
Zhijie Yao ◽  
Jiayu Yu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Three Dimensional ◽  
Glucose Biosensor ◽  
Detection Sensitivity ◽  
Nanoporous Structure ◽  
Glucose Detection ◽  
Glucose Content ◽  
Wide Range ◽  
Nanoporous Platinum

Abstract Microelectrode glucose biosensor based on three-dimensional hybrid nanoporous platinum/graphene oxide nanostructure was developed for rapid glucose detection of tomato and cucumber fruits. The nanostructure was fabricated by a two-step modification method on microelectrode for loading a larger amount of glucose oxidase. The nanoporous structure was prepared on the surface of the platinum microelectrode by electrochemical etching, and then graphene oxide was deposited on the prepared nanoporous electrode by electrochemical deposition. The nanoprorous platinum/graphene oxide nanostructure had the advantage of improving the effective surface area of the electrode and the loading quantity of glucose oxidase. As a result, the biosensor achieved a wide range of 0.1-20.0 mM in glucose detection, which had the ability to accurately detect the glucose content. It was found that the three-dimensional hybrid nanostructure on the electrode surface realized the rapid direct electrochemistry of glucose oxidase. Therefore, the biosensor achieved high glucose detection sensitivity (11.64 μA mM -1cm -2), low detection limit (13 μM) and rapid response time (reaching 95% steady-state response within 3 seconds), when calibrating in glucose standard solution. In agricultural application, the as-prepared biosensor was employed to detect the glucose concentration of tomato and cucumber samples. The results showed that the relative deviation of this method was less than 5% when compared with that of HPLC, implying high accuracy of the presented biosensor in glucose detection in plants.

Temperature-induced amperometric glucose biosensor based on a poly(N-vinylcaprolactam)/graphene oxide composite film

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 1960-1967 ◽  
Author(s):  
Chao Chen ◽  
Pengcheng Zhao ◽  
Meijun Ni ◽  
Chunyan Li ◽  
Yixi Xie ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Composite Film ◽  
Glucose Oxidase ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Glucose Biosensor ◽  
Carbon Electrode ◽  
Oxide Composite

A temperature-induced sensing film consisting of poly(N-vinylcaprolactam) (PVCL), graphene oxide (GO) and glucose oxidase (GOD) was fabricated and used to modify a glassy carbon electrode (GCE).

scholarly journals Electrochemical Response of Glucose Oxidase Adsorbed on Laser-Induced Graphene

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1893
Author(s):  
Sónia O. Pereira ◽  
Nuno F. Santos ◽  
Alexandre F. Carvalho ◽  
António J. S. Fernandes ◽  
Florinda M. Costa
Keyword(s):  
Electron Transfer ◽  
Glucose Oxidase ◽  
Ph Dependence ◽  
High Surface ◽  
High Surface Area ◽  
Cost Effective ◽  
Great Promise ◽  
Glucose Detection ◽  
Half Wave ◽  
Electrochemical Transducers

Carbon-based electrodes have demonstrated great promise as electrochemical transducers in the development of biosensors. More recently, laser-induced graphene (LIG), a graphene derivative, appears as a great candidate due to its superior electron transfer characteristics, high surface area and simplicity in its synthesis. The continuous interest in the development of cost-effective, more stable and reliable biosensors for glucose detection make them the most studied and explored within the academic and industry community. In this work, the electrochemistry of glucose oxidase (GOx) adsorbed on LIG electrodes is studied in detail. In addition to the well-known electroactivity of free flavin adenine dinucleotide (FAD), the cofactor of GOx, at the expected half-wave potential of −0.490 V vs. Ag/AgCl (1 M KCl), a new well-defined redox pair at 0.155 V is observed and shown to be related to LIG/GOx interaction. A systematic study was undertaken in order to understand the origin of this activity, including scan rate and pH dependence, along with glucose detection tests. Two protons and two electrons are involved in this reaction, which is shown to be sensitive to the concentration of glucose, restraining its origin to the electron transfer from FAD in the active site of GOx to the electrode via direct or mediated by quinone derivatives acting as mediators.

Immobilization of Glucose Oxidase on Plasma-Treated Polyethylene for Non-Invasive Glucose Detection

2021 ◽  
pp. 115509
Author(s):  
Georgina Fabregat ◽  
Sonia Lanzalaco ◽  
Jules Aït Saïd ◽  
Xavier Muñoz-Pascual ◽  
Jordi Llorca ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Glucose Detection ◽  
Non Invasive

Evaluation of Hierarchical Glucose Oxidase/Co3Mn-CO3 LDH Modified Electrodes for Glucose Detection

2021 ◽  
pp. 138050
Author(s):  
Hani Farhat ◽  
Joel Célier ◽  
Claude Forano ◽  
Christine Mousty
Keyword(s):  
Glucose Oxidase ◽  
Modified Electrodes ◽  
Glucose Detection
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