A smart device for label-free and real-time detection of gene point mutations based on the high dark phase contrast of vapor condensation

Lab on a Chip ◽  
2015 ◽  
Vol 15 (19) ◽  
pp. 3891-3896 ◽  
Author(s):  
Junqi Zhang ◽  
Rongxin Fu ◽  
Liping Xie ◽  
Qi Li ◽  
Wenhan Zhou ◽  
...  
Keyword(s):  
Real Time ◽  
Point Mutation ◽  
Phase Contrast ◽  
Point Mutations ◽  
Vapor Condensation ◽  
Smart Device ◽  
Dark Phase ◽  
Label Free ◽  
Real Time Detection

A smart device for label-free and real-time detection of gene point mutation-related diseases was developed based on the high dark phase contrast of vapor condensation.

Label-Free and Regenerable Aptasensor for Real-Time Detection of Cadmium(II) by Dual Polarization Interferometry

2020 ◽  
Vol 92 (14) ◽  
pp. 10007-10015 ◽  
Author(s):  
Yu Xue ◽  
Yu Wang ◽  
Shuang Wang ◽  
Mengxia Yan ◽  
Jianshe Huang ◽  
...  
Keyword(s):  
Real Time ◽  
Label Free ◽  
Dual Polarization ◽  
Polarization Interferometry ◽  
Dual Polarization Interferometry ◽  
Real Time Detection

scholarly journals Real-time halo correction in phase contrast imaging

2017 ◽  
Author(s):  
Mikhail E. Kandel ◽  
Michael Fanous ◽  
Catherine Best-Popescu ◽  
Gabriel Popescu
Keyword(s):  
Real Time ◽  
Phase Contrast ◽  
A Priori ◽  
Spatial Coherence ◽  
Low Frequency ◽  
Nondestructive Method ◽  
Label Free ◽  
Contrast Imaging ◽  
Tissue Slices ◽  
Time Operation

AbstractAs a label-free, nondestructive method, phase contrast is by far the most popular microscopy technique for routine inspection of cell cultures. Yet, features of interest such as extensions near cell bodies are often obscured by a glow, which came to be known as the halo. Advances in modeling image formation have shown that this artifact is due to the limited spatial coherence of the illumination. Yet, the same incoherent illumination is responsible for superior sensitivity to fine details in the phase contrast geometry. Thus, there exists a trade-off between high-detail (incoherent) and low-detail (coherent) imaging systems. In this work, we propose a method to break this dichotomy, by carefully mixing corrected low-frequency and high-frequency data in a way that eliminates the edge effect. Specifically, our technique is able to remove halo artifacts at video rates, requiring no manual interaction ora prioripoint spread function measurements. To validate our approach, we imaged standard spherical beads, sperm cells, tissue slices, and red blood cells. We demonstrate the real-time operation with a time evolution study of adherent neuron cultures whose neurites are revealed by our halo correction. We show that with our novel technique, we can quantify cell growth in large populations, without the need for thresholds and calibration.

scholarly journals Real-time label-free microscopy with adjustable phase-contrast

2020 ◽  
Vol 28 (19) ◽  
pp. 27524
Author(s):  
Amber Galeana ◽  
Rosario Porras-Aguilar
Keyword(s):  
Real Time ◽  
Phase Contrast ◽  
Label Free

Nanophotonics based label free detection mechanism for real-time monitoring of interleukin-6

Nanoscale ◽  
2020 ◽  
Vol 12 (16) ◽  
pp. 9194-9207 ◽  
Author(s):  
Munezza A. Khan ◽  
Mohammad Mujahid ◽  
Say Chye Joachim Loo ◽  
Vidya N. Chamundeswari
Keyword(s):  
Photonic Crystals ◽  
Real Time ◽  
Interleukin 6 ◽  
High Fidelity ◽  
Label Free ◽  
Real Time Monitoring ◽  
Detection Mechanism ◽  
Label Free Detection ◽  
Real Time Detection

Magneto-photonic crystals/MPCs are promising candidates for devising high-fidelity embedded biosensor systems which offer facile & real time detection of diagnostic proteins.

scholarly journals Real-time detection of telomerase activity in cancer cells using a label-free electrochemical impedimetric biosensing microchip

RSC Advances ◽  
2014 ◽  
Vol 4 (94) ◽  
pp. 52357-52365 ◽  
Author(s):  
Lisandro Cunci ◽  
Marina Martinez Vargas ◽  
Roman Cunci ◽  
Ramon Gomez-Moreno ◽  
Ivan Perez ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Real Time ◽  
Telomerase Activity ◽  
Label Free ◽  
Real Time Detection ◽  
Impedimetric Biosensor

Impedimetric biosensor microchip for the detection of telomerase activity as a biomarker for cancer.

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