Real-time detection of small and large molecules using a porous silicon grating-coupled Bloch surface wave label-free biosensor

2013 ◽  
Author(s):  
Gilberto A. Rodriguez ◽  
Judson D. Ryckman ◽  
Yang Jiao ◽  
Robert L. Fuller ◽  
Sharon M. Weiss
Keyword(s):  
Surface Wave ◽  
Porous Silicon ◽  
Real Time ◽  
Label Free ◽  
Large Molecules ◽  
Bloch Surface Wave ◽  
Real Time Detection

Label-Free and Regenerable Aptasensor for Real-Time Detection of Cadmium(II) by Dual Polarization Interferometry

2020 ◽  
Vol 92 (14) ◽  
pp. 10007-10015 ◽  
Author(s):  
Yu Xue ◽  
Yu Wang ◽  
Shuang Wang ◽  
Mengxia Yan ◽  
Jianshe Huang ◽  
...  
Keyword(s):  
Real Time ◽  
Label Free ◽  
Dual Polarization ◽  
Polarization Interferometry ◽  
Dual Polarization Interferometry ◽  
Real Time Detection

Combining label-free and fluorescence operation of Bloch surface wave optical sensors

2014 ◽  
Vol 39 (10) ◽  
pp. 2947 ◽  
Author(s):  
Alberto Sinibaldi ◽  
Antonio Fieramosca ◽  
Riccardo Rizzo ◽  
Aleksei Anopchenko ◽  
Norbert Danz ◽  
...  
Keyword(s):  
Surface Wave ◽  
Optical Sensors ◽  
Label Free ◽  
Bloch Surface Wave

scholarly journals A lectin-coupled porous silicon-based biosensor: label-free optical detection of bacteria in a real-time mode

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mona Yaghoubi ◽  
Fereshteh Rahimi ◽  
Babak Negahdari ◽  
Ali Hossein Rezayan ◽  
Azizollah Shafiekhani
Keyword(s):  
Fourier Transform ◽  
Porous Silicon ◽  
Real Time ◽  
Limit Of Detection ◽  
Principal Component ◽  
The Other ◽  
Detection Methods ◽  
Carbohydrate Binding ◽  
Label Free ◽  
E Coli

Abstract Accuracy and speed of detection, along with technical and instrumental simplicity, are indispensable for the bacterial detection methods. Porous silicon (PSi) has unique optical and chemical properties which makes it a good candidate for biosensing applications. On the other hand, lectins have specific carbohydrate-binding properties and are inexpensive compared to popular antibodies. We propose a lectin-conjugated PSi-based biosensor for label-free and real-time detection of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by reflectometric interference Fourier transform spectroscopy (RIFTS). We modified meso-PSiO2 (10–40 nm pore diameter) with three lectins of ConA (Concanavalin A), WGA (Wheat Germ Agglutinin), and UEA (Ulex europaeus agglutinin) with various carbohydrate specificities, as bioreceptor. The results showed that ConA and WGA have the highest binding affinity for E. coli and S. aureus respectively and hence can effectively detect them. This was confirmed by 6.8% and 7.8% decrease in peak amplitude of fast Fourier transform (FFT) spectra (at 105 cells mL−1 concentration). A limit of detection (LOD) of about 103 cells mL−1 and a linear response range of 103 to 105 cells mL−1 were observed for both ConA-E. coli and WGA-S. aureus interaction platforms that are comparable to the other reports in the literature. Dissimilar response patterns among lectins can be attributed to the different bacterial cell wall structures. Further assessments were carried out by applying the biosensor for the detection of Klebsiella aerogenes and Bacillus subtilis bacteria. The overall obtained results reinforced the conjecture that the WGA and ConA have a stronger interaction with Gram-positive and Gram-negative bacteria, respectively. Therefore, it seems that specific lectins can be suggested for bacterial Gram-typing or even serotyping. These observations were confirmed by the principal component analysis (PCA) model.

scholarly journals Protease detection using a porous silicon based Bloch surface wave optical biosensor

2010 ◽  
Vol 18 (14) ◽  
pp. 15174 ◽  
Author(s):  
Hong Qiao ◽  
Bin Guan ◽  
J. Justin Gooding ◽  
Peter J Reece
Keyword(s):  
Surface Wave ◽  
Porous Silicon ◽  
Optical Biosensor ◽  
Bloch Surface Wave ◽  
Silicon Based

The analytical performance of a porous silicon Bloch surface wave biosensors as protease biosensor

2015 ◽  
Vol 211 ◽  
pp. 469-475 ◽  
Author(s):  
Hong Qiao ◽  
Alexander H. Soeriyadi ◽  
Bin Guan ◽  
Peter J. Reece ◽  
J. Justin Gooding
Keyword(s):  
Surface Wave ◽  
Porous Silicon ◽  
Analytical Performance ◽  
Bloch Surface Wave
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