X-chromosome inactivation in extra-embryonic membranes of diploid parthenogenetic mouse embryos demonstrated by differential staining

Nature ◽  
1980 ◽  
Vol 288 (5787) ◽  
pp. 172-173 ◽  
Author(s):  
S. Rastan ◽  
M. H. Kaufman ◽  
A. H. Handyside ◽  
M. F. Lyon
Keyword(s):  
X Chromosome ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
Chromosome Inactivation ◽  
Differential Staining ◽  
Parthenogenetic Mouse Embryos

X chromosome inactivation in diploid parthenogenetic mouse embryos

Nature ◽  
1978 ◽  
Vol 271 (5645) ◽  
pp. 547-549 ◽  
Author(s):  
MATTHEW H. KAUFMAN ◽  
MARIJA GUC-CUBRILO ◽  
MARY F. LYON
Keyword(s):  
X Chromosome ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
Chromosome Inactivation ◽  
Parthenogenetic Mouse Embryos

scholarly journals Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation.

1990 ◽  
Vol 10 (9) ◽  
pp. 4987-4989 ◽  
Author(s):  
J Singer-Sam ◽  
M Grant ◽  
J M LeBon ◽  
K Okuyama ◽  
V Chapman ◽  
...  
Keyword(s):  
Dna Methylation ◽  
X Chromosome ◽  
Inner Cell Mass ◽  
Cpg Island ◽  
Cell Mass ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
Chromosome Inactivation ◽  
Individual Mouse ◽  
Polymerase Chain

A HpaII-PCR assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is less than or equal to 10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days; about the time of X-chromosome inactivation of the inner cell mass.

Non-random X-chromosome inactivation in mouse X-autosome translocation embryos—location of the inactivation centre

Development ◽  
1983 ◽  
Vol 78 (1) ◽  
pp. 1-22
Author(s):  
Sohaila Rastan
Keyword(s):  
Simple Model ◽  
X Chromosome ◽  
X Chromosome Inactivation ◽  
Chromosome Inactivation ◽  
Differential Staining ◽  
Female Embryo ◽  
Reciprocal Translocations ◽  
Inactive X Chromosome ◽  
Autosome Translocation ◽  
Chromosome Material

X-chromosome inactivation was investigated cytologically using the modified Kanda method which differentially stains inactive X-chromosome material at metaphase in balanced 13½-day female embryos heterozygous for four X-autosome rearrangements, reciprocal translocations T(X;4)37H, T(X;11)38H and T(X;16)16H (Searle's translocation) and the insertion translocation Is(7;X)1Ct (Cattanach's translocation). In all cases non-random inactivation was found. In the reciprocal translocation heterozygotes only one translocation product ever showed Kanda staining. In addition in a proportion of cells from T(X;4)37H, T(X;11)38H and Is(7;X)1Ct the Kanda staining revealed differential staining of X-chromosome material and attached autosomal material within the translocation product. In a study of 8½-day female embryos doubly heterozygous for Searle's translocation and Cattanach's translocation two unbalanced types of embryo were found. In one type of unbalanced female embryo of the karyotype 40(X(7)/X16;16/16) no inactivated X-chromosomal material is found. A second unbalanced type of female embryo, of the presumptive karyotype 40(X(7)/XN;16x/l6) was found in which two inactivated chromosomes were present in the majority of metaphase spreads. A simple model for the initiation of X-chromosome inactivation based on the presence of a single inactivation centre distal to the breakpoint in Searle's translocation explains these findings.

Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation

1990 ◽  
Vol 10 (9) ◽  
pp. 4987-4989
Author(s):  
J Singer-Sam ◽  
M Grant ◽  
J M LeBon ◽  
K Okuyama ◽  
V Chapman ◽  
...  
Keyword(s):  
Dna Methylation ◽  
X Chromosome ◽  
Inner Cell Mass ◽  
Cpg Island ◽  
Cell Mass ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
Chromosome Inactivation ◽  
Individual Mouse ◽  
Polymerase Chain

A HpaII-PCR assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is less than or equal to 10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days; about the time of X-chromosome inactivation of the inner cell mass.

scholarly journals Ftx is dispensable for imprinted X-chromosome inactivation in preimplantation mouse embryos

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Miki Soma ◽  
Yoshitaka Fujihara ◽  
Masaru Okabe ◽  
Fumitoshi Ishino ◽  
Shin Kobayashi
Keyword(s):  
X Chromosome ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
Chromosome Inactivation ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse
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