scholarly journals Ftx is dispensable for imprinted X-chromosome inactivation in preimplantation mouse embryos

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Miki Soma ◽  
Yoshitaka Fujihara ◽  
Masaru Okabe ◽  
Fumitoshi Ishino ◽  
Shin Kobayashi
Keyword(s):  
X Chromosome ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
Chromosome Inactivation ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse

scholarly journals Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation.

1990 ◽  
Vol 10 (9) ◽  
pp. 4987-4989 ◽  
Author(s):  
J Singer-Sam ◽  
M Grant ◽  
J M LeBon ◽  
K Okuyama ◽  
V Chapman ◽  
...  
Keyword(s):  
Dna Methylation ◽  
X Chromosome ◽  
Inner Cell Mass ◽  
Cpg Island ◽  
Cell Mass ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
Chromosome Inactivation ◽  
Individual Mouse ◽  
Polymerase Chain

A HpaII-PCR assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is less than or equal to 10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days; about the time of X-chromosome inactivation of the inner cell mass.

Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation

1990 ◽  
Vol 10 (9) ◽  
pp. 4987-4989
Author(s):  
J Singer-Sam ◽  
M Grant ◽  
J M LeBon ◽  
K Okuyama ◽  
V Chapman ◽  
...  
Keyword(s):  
Dna Methylation ◽  
X Chromosome ◽  
Inner Cell Mass ◽  
Cpg Island ◽  
Cell Mass ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
Chromosome Inactivation ◽  
Individual Mouse ◽  
Polymerase Chain

A HpaII-PCR assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is less than or equal to 10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days; about the time of X-chromosome inactivation of the inner cell mass.

X-chromosome activity in preimplantation mouse embryos from XX and XO mothers

Development ◽  
1978 ◽  
Vol 46 (1) ◽  
pp. 53-64
Author(s):  
Marilyn Monk ◽  
Mary Harper
Keyword(s):  
X Chromosome ◽  
Bimodal Distribution ◽  
Mouse Embryos ◽  
Activity Levels ◽  
Cell Stage ◽  
X Chromosomes ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
Morula Stage ◽  
The Mean

Embryos from XO female mice begin development with half the activity levels of an enzyme (HPRT) coded for by a gene on the X chromosome, compared with embryos from XX females. Groups of unfertilized eggs and individual embryos at the 8-cell, morula and blastocyst stages were assayed for HPRT activity. An autosomally coded enzyme (APRT) was assayed simultaneously in the same reaction mix as a control. There is a substantial increase in HPRT activity by the 8-cell stage. However, the mean activity of HPRT in embryos of XO mothers remains half that in embryos of XX mothers. This suggests a significant maternally inherited component of HPRT activity in 8-cell embryos. By the 9- to 16-cell morula stage the HPRT activities in the two groups of embryos become similar due, presumably, to a transition to embryo-coded activity; HPRT activities in individual morulae from XX mothers show a bimodal distribution consistent with the hypothesis that both X-chromosomes are active in XX embryos at this stage.

X chromosome inactivation in diploid parthenogenetic mouse embryos

Nature ◽  
1978 ◽  
Vol 271 (5645) ◽  
pp. 547-549 ◽  
Author(s):  
MATTHEW H. KAUFMAN ◽  
MARIJA GUC-CUBRILO ◽  
MARY F. LYON
Keyword(s):  
X Chromosome ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
Chromosome Inactivation ◽  
Parthenogenetic Mouse Embryos
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