Primary and secondary nonrandom X chromosome inactivation in early female mouse embryos carrying Searle's translocation T(X; 16)16H

Chromosoma ◽  
1980 ◽  
Vol 81 (3) ◽  
pp. 439-459 ◽  
Author(s):  
Nobuo Takagi
Keyword(s):  
X Chromosome ◽  
Female Mouse ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
Chromosome Inactivation

Both X chromosomes function before visible X-chromosome inactivation in female mouse embryos

Nature ◽  
1978 ◽  
Vol 274 (5670) ◽  
pp. 500-503 ◽  
Author(s):  
CHARLES J. EPSTEIN ◽  
SANDRA SMITH ◽  
BRUCE TRAVIS ◽  
GEORGIANNE TUCKER
Keyword(s):  
X Chromosome ◽  
Female Mouse ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
Chromosome Inactivation ◽  
X Chromosomes

Timing of X-chromosome inactivation in postimplantation mouse embryos

Development ◽  
1982 ◽  
Vol 71 (1) ◽  
pp. 11-24
Author(s):  
Sohaila Rastan
Keyword(s):  
X Chromosome ◽  
Mouse Embryo ◽  
Female Mouse ◽  
Primitive Streak ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
X Inactivation ◽  
Chromosome Inactivation ◽  
Inactive X Chromosome ◽  
Dark Staining

The onset of X-chromosome inactivation was investigated cytologically in postimplantation female mouse embryos of age 5½, 6½ and 7½ days post-coitum (d.p.c.) and in the isolated epiblasts of 6 d.p.c. embryos before primitive streak formation using a heat/hypotonic technique to reveal the inactive X chromosome by differentially dark staining with Giemsa. The results indicate that X inactivation has taken place in all the cells of the so-called ‘undifferentiated’ epiblast by 6 d.p.c. before primitive streak formation. Further evidence is presented to suggest that X inactivation is complete in all cells of the mouse embryo by 5½ d.p.c.

scholarly journals Mouse endogenous X-linked genes do not show lineage-specific delayed inactivation during development

1995 ◽  
Vol 65 (3) ◽  
pp. 223-227 ◽  
Author(s):  
Jeanne M. Lebon ◽  
Patrick P. L. Tam ◽  
Judith Singer-Sam ◽  
Arthur D. Riggs ◽  
Seong-Seng Tan
Keyword(s):  
X Chromosome ◽  
Female Mouse ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
Primer Extension ◽  
Rt Pcr ◽  
Single Nucleotide ◽  
Allele Specific ◽  
Endogenous Genes ◽  
Single Nucleotide Primer Extension

SummaryX chromosome inactivation (XCI) has been assumed to be complete in all cells of female mouse embryos at about 6 d post coitum (dpc). However, a recent study on β-galactosidase expression of an X-linkedlacZtransgene suggests that XCI is probably not complete several days after this time in some lineages. To help resolve this issue, we analysed XCI in embryos which carry the T(X;16)16H (Searle's) translocation and are heterozygous at the X-linkedHprtandPgk-1genes. The quantitative RT-PCR single nucleotide primer extension (SNuPE) assay was used to measureHprtandPgk-1allele-specific transcripts in embryos 9·5 dpc. No transcripts from the normal X chromosome were found in any of the tissues tested, indicating that inactivation was complete for these endogenous genes.

scholarly journals SPEN is required for Xist upregulation during initiation of X chromosome inactivation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Teresa Robert-Finestra ◽  
Beatrice F. Tan ◽  
Hegias Mira-Bontenbal ◽  
Erika Timmers ◽  
Cristina Gontan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
X Chromosome ◽  
Female Mouse ◽  
Antisense Transcript ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
X Chromosome Inactivation ◽  
Chromosome Inactivation ◽  
Chromatin Remodelers

AbstractAt initiation of X chromosome inactivation (XCI), Xist is monoallelically upregulated from the future inactive X (Xi) chromosome, overcoming repression by its antisense transcript Tsix. Xist recruits various chromatin remodelers, amongst them SPEN, which are involved in silencing of X-linked genes in cis and establishment of the Xi. Here, we show that SPEN plays an important role in initiation of XCI. Spen null female mouse embryonic stem cells (ESCs) are defective in Xist upregulation upon differentiation. We find that Xist-mediated SPEN recruitment to the Xi chromosome happens very early in XCI, and that SPEN-mediated silencing of the Tsix promoter is required for Xist upregulation. Accordingly, failed Xist upregulation in Spen−/− ESCs can be rescued by concomitant removal of Tsix. These findings indicate that SPEN is not only required for the establishment of the Xi, but is also crucial in initiation of the XCI process.

scholarly journals Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation.

1990 ◽  
Vol 10 (9) ◽  
pp. 4987-4989 ◽  
Author(s):  
J Singer-Sam ◽  
M Grant ◽  
J M LeBon ◽  
K Okuyama ◽  
V Chapman ◽  
...  
Keyword(s):  
Dna Methylation ◽  
X Chromosome ◽  
Inner Cell Mass ◽  
Cpg Island ◽  
Cell Mass ◽  
X Chromosome Inactivation ◽  
Mouse Embryos ◽  
Chromosome Inactivation ◽  
Individual Mouse ◽  
Polymerase Chain

A HpaII-PCR assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is less than or equal to 10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days; about the time of X-chromosome inactivation of the inner cell mass.

scholarly journals Reduced proportion of Purkinje cells expressing paternally derived mutant Mecp2308 allele in female mouse cerebellum is not due to a skewed primary pattern of X-chromosome inactivation

2005 ◽  
Vol 14 (13) ◽  
pp. 1851-1861 ◽  
Author(s):  
Catherine M. Watson ◽  
Gregory J. Pelka ◽  
Tatiana Radziewic ◽  
Mona D. Shahbazian ◽  
John Christodoulou ◽  
...  
Keyword(s):  
Purkinje Cells ◽  
X Chromosome ◽  
Female Mouse ◽  
X Chromosome Inactivation ◽  
Chromosome Inactivation ◽  
Mouse Cerebellum ◽  
Primary Pattern
Sign in / Sign up

Export Citation Format

Share Document