Click Chemistry as a Reliable Method for the High-Density Postsynthetic Functionalization of Alkyne-Modified DNA

2006 ◽  
Vol 8 (17) ◽  
pp. 3639-3642 ◽  
Author(s):  
Johannes Gierlich ◽  
Glenn A. Burley ◽  
Philipp M. E. Gramlich ◽  
David M. Hammond ◽  
Thomas Carell
Keyword(s):  
Click Chemistry ◽  
Reliable Method ◽  
High Density ◽  
Modified Dna

High Density Orthogonal Surface Immobilization via Photoactivated Copper-Free Click Chemistry

2010 ◽  
Vol 132 (32) ◽  
pp. 11024-11026 ◽  
Author(s):  
Sara V. Orski ◽  
Andrei A. Poloukhtine ◽  
Selvanathan Arumugam ◽  
Leidong Mao ◽  
Vladimir V. Popik ◽  
...  
Keyword(s):  
Click Chemistry ◽  
High Density ◽  
Surface Immobilization ◽  
Orthogonal Surface

High-Density DNA Functionalization by a Combination of Cu-Catalyzed and Cu-Free Click Chemistry

2010 ◽  
Vol 16 (23) ◽  
pp. 6877-6883 ◽  
Author(s):  
Katrin Gutsmiedl ◽  
Danila Fazio ◽  
Thomas Carell
Keyword(s):  
Click Chemistry ◽  
High Density ◽  
Dna Functionalization

Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry

2007 ◽  
Vol 13 (34) ◽  
pp. 9486-9494 ◽  
Author(s):  
Johannes Gierlich ◽  
Katrin Gutsmiedl ◽  
Philipp M. E. Gramlich ◽  
Alexandra Schmidt ◽  
Glenn A. Burley ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Modified Dna

scholarly journals A triazole linkage that mimics the DNA phosphodiester group in living systems

2015 ◽  
Vol 48 (4) ◽  
pp. 429-436 ◽  
Author(s):  
Afaf H. El-Sagheer ◽  
Tom Brown
Keyword(s):  
Click Chemistry ◽  
Chemical Reaction ◽  
Chemical Process ◽  
Living Systems ◽  
Complementary Dna ◽  
Chemically Modified ◽  
Dna And Rna ◽  
Modified Dna ◽  
Dna Strands ◽  
Phosphodiester Group

AbstractWe describe the development of a chemical process based on the CuAAC reaction (click chemistry) to ligate DNA strands and produce an unnatural triazole backbone linkage. The chemical reaction is templated by a complementary DNA splint which accelerates the reaction and provides the required specificity. The resultant 1,4-triazole linkage is read through by DNA and RNA polymerases and is biocompatible in bacterial and human cells. This work has implications for the synthesis of chemically modified genes and other large modified DNA and RNA constructs.

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