Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry

2007 ◽  
Vol 13 (34) ◽  
pp. 9486-9494 ◽  
Author(s):  
Johannes Gierlich ◽  
Katrin Gutsmiedl ◽  
Philipp M. E. Gramlich ◽  
Alexandra Schmidt ◽  
Glenn A. Burley ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Modified Dna

Click Chemistry as a Reliable Method for the High-Density Postsynthetic Functionalization of Alkyne-Modified DNA

2006 ◽  
Vol 8 (17) ◽  
pp. 3639-3642 ◽  
Author(s):  
Johannes Gierlich ◽  
Glenn A. Burley ◽  
Philipp M. E. Gramlich ◽  
David M. Hammond ◽  
Thomas Carell
Keyword(s):  
Click Chemistry ◽  
Reliable Method ◽  
High Density ◽  
Modified Dna

scholarly journals A triazole linkage that mimics the DNA phosphodiester group in living systems

2015 ◽  
Vol 48 (4) ◽  
pp. 429-436 ◽  
Author(s):  
Afaf H. El-Sagheer ◽  
Tom Brown
Keyword(s):  
Click Chemistry ◽  
Chemical Reaction ◽  
Chemical Process ◽  
Living Systems ◽  
Complementary Dna ◽  
Chemically Modified ◽  
Dna And Rna ◽  
Modified Dna ◽  
Dna Strands ◽  
Phosphodiester Group

AbstractWe describe the development of a chemical process based on the CuAAC reaction (click chemistry) to ligate DNA strands and produce an unnatural triazole backbone linkage. The chemical reaction is templated by a complementary DNA splint which accelerates the reaction and provides the required specificity. The resultant 1,4-triazole linkage is read through by DNA and RNA polymerases and is biocompatible in bacterial and human cells. This work has implications for the synthesis of chemically modified genes and other large modified DNA and RNA constructs.

Click Chemistry Expedited Radiosynthesis: Sulfur [18F]fluoride Exchange of Aryl Fluorosulfates

2019 ◽  
Author(s):  
Qinheng Zheng ◽  
Hongtao Xu ◽  
Hua Wang ◽  
Wen-Ge Han Du ◽  
Nan Wang ◽  
...  
Keyword(s):  
Click Chemistry ◽  
High Performance ◽  
Isotopic Exchange ◽  
Tumor Imaging ◽  
Radiochemical Yield ◽  
Exchange Method ◽  
Molar Activity ◽  
Positron Emission ◽  
Sulfur Fluoride

The lack of simple, efficient [<sup>18</sup>F]fluorination processes and new target-specific organofluorine probes remains the major challenge of fluorine-18-based positron emission tomography (PET). We report here a fast isotopic exchange method for the radiosynthesis of aryl [<sup>18</sup>F]fluorosulfate based PET agents enabled by the emerging sulfur fluoride exchange (SuFEx) click chemistry. The method has been applied to the fully-automated <sup>18</sup>F-radiolabeling of twenty-five structurally diverse aryl fluorosulfates with excellent radiochemical yield (83–100%) and high molar activity (up to 281 GBq µmol<sup>–1</sup>) at room temperature in 30 seconds. The purification of radiotracers requires no time-consuming high-performance liquid chromatography (HPLC), but rather a simple cartridge filtration. The utility of aryl [<sup>18</sup>F]fluorosulfate is demonstrated by the <i>in vivo</i> tumor imaging by targeting poly(ADP-ribose) polymerase 1 (PARP1).

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