Gold Nanorods as Absorption Contrast Agents for the Noninvasive Detection of Arterial Vascular Disorders Based on Diffusion Reflection Measurements

Nano Letters ◽  
2014 ◽  
Vol 14 (5) ◽  
pp. 2681-2687 ◽  
Author(s):  
Rinat Ankri ◽  
Dorit Leshem-Lev ◽  
Dror Fixler ◽  
Rachela Popovtzer ◽  
Menachem Motiei ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Gold Nanorods ◽  
Noninvasive Detection ◽  
Vascular Disorders ◽  
Diffusion Reflection ◽  
Absorption Contrast

scholarly journals Tissue-Like Phantoms as a Platform for Inserted Fluorescence Nano-Probes

2016 ◽  
Author(s):  
Tsviya Nayhoz ◽  
Eran A. Barnoy ◽  
Dror Fixler
Keyword(s):  
Optical Properties ◽  
Contrast Agents ◽  
Fluorescence Lifetime ◽  
Gold Nanorods ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Reflection Method ◽  
Lifetime Imaging ◽  
Live Tissue ◽  
Diffusion Reflection

Tissue-like phantoms are widely used as a model for mimicking the optical properties of live tissue. This paper presents the results of a diffusion reflection method as well as fluorescence lifetime imaging microscopy measurements of fluorescein-conjugated gold nanorods in solution as well as inserted in solid tissue-imitating phantoms. A lack of consistency between the fluorescence lifetime results of the solutions and the phantoms raises a question about the ability of tissue-like phantoms to maintain the optical properties of inserted contrast agents.

Polymeric/Inorganic Multifunctional Nanoparticles for Simultaneous Drug Delivery and Visualization

2010 ◽  
Vol 1257 ◽  
Author(s):  
Andrea Fornara ◽  
Alberto Recalenda ◽  
Jian Qin ◽  
Abhilash Sugunan ◽  
Fei Ye ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Fluorescence Microscopy ◽  
Contrast Agents ◽  
Gold Nanorods ◽  
In Vitro Cytotoxicity ◽  
In Vivo Studies ◽  
Multifunctional Nanoparticles ◽  
Cytotoxicity Studies

AbstractNanoparticles consisting of different biocompatible materials are attracting a lot of interest in the biomedical area as useful tools for drug delivery, photo-therapy and contrast enhancement agents in MRI, fluorescence and confocal microscopy. This work mainly focuses on the synthesis of polymeric/inorganic multifunctional nanoparticles (PIMN) based on biocompatible di-block copolymer poly(L,L-lactide-co-ethylene glycol) (PLLA-PEG) via an emulsion-evaporation method. Besides containing a hydrophobic drug (Indomethacin), these polymeric nanoparticles incorporate different visualization agents such as superparamagnetic iron oxide nanoparticles (SPION) and fluorescent Quantum Dots (QDs) that are used as contrast agents for Magnetic Resonance Imaging (MRI) and fluorescence microscopy together. Gold Nanorods are also incorporated in such nanostructures to allow simultaneous visualization and photodynamic therapy. MRI studies are performed with different loading of SPION into PIMN, showing an enhancement in T2 contrast superior to commercial contrast agents. Core-shell QDs absorption and emission spectra are recorded before and after their loading into PIMN. With these polymeric/inorganic multifunctional nanoparticles, both MRI visualization and confocal fluorescence microscopy studies can be performed. Gold nanorods are also synthesized and incorporated into PIMN without changing their longitudinal absorption peak usable for lased excitation and phototherapy. In-vitro cytotoxicity studies have also been performed to confirm the low cytotoxicity of PIMN for further in-vivo studies.

Biocompatible PEGylated gold nanorods as colored contrast agents for targetedin vivocancer applications

2010 ◽  
Vol 21 (31) ◽  
pp. 315101 ◽  
Author(s):  
Atcha Kopwitthaya ◽  
Ken-Tye Yong ◽  
Rui Hu ◽  
Indrajit Roy ◽  
Hong Ding ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Gold Nanorods

scholarly journals Micro-CT Imaging of RGD-Conjugated Gold Nanorods Targeting TumorIn Vivo

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Xiaochao Qu ◽  
Xiaoxiao Li ◽  
Jingning Liang ◽  
Yanran Wang ◽  
Muhan Liu ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Gold Nanorods ◽  
Tumor Targeting ◽  
Rgd Peptide ◽  
Ct Imaging ◽  
Micro Ct ◽  
Imaging Capability ◽  
U87 Cells ◽  
Ht 29

Gold nanomaterials as computed tomography (CT) contrast agents at lower X-ray dosage to get a higher contrast have advantages of longer imaging time and lower toxic side effects compared to current contrast agents. As a receptor for Cyclo (Arg-Gly-Asp-D-Phe-Lys) (RGD) peptide, integrinαvβ3is overexpressed on some tumor cells and tumor neovasculature. In this paper, we conjugated the RGD peptide on the surface of gold nanorods (AuNRs), designated as RGD-AuNRs, a promising candidate in applications such as tumor targeting and imaging capability for micro-CT imaging. Integrinαvβ3-positive U87 cells and integrinαvβ3-negative HT-29 cells were chosen to establish animal models relatedly and then texted the tumor targeting ability and imaging capability of RGD-AuNRsin vitroandin vivo. The MTT assay and stability measurement showed that RGD-conjugation eliminated their cytotoxicity and improved their biocompatibility and stability. Dark-field imaging of U87 cells and HT-29 cells testified the binding affinities and uptake abilities of RGD-AuNRs, and the results showed that RGD-AuNRs were more specifical to U87 cells. The enhanced micro-CT imaging contrast of intramuscular and subcutaneous injection illustrated the feasibility of RGD-AuNRs to be contrast agents. Furthermore, the micro-CT imaging of targeting U87 and HT-29 tumor models verified the targeting abilities of RGD-AuNRs.

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