RP-HPLC Analysis of the Phenolic Compounds of Plant Extracts. Investigation of Their Antioxidant Capacity and Antimicrobial Activity

2005 ◽  
Vol 53 (4) ◽  
pp. 1190-1195 ◽  
Author(s):  
C. Proestos ◽  
N. Chorianopoulos ◽  
G.-J. E. Nychas ◽  
M. Komaitis
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Plant Extracts ◽  
Hplc Analysis ◽  
Rp Hplc

RP-HPLC analysis of phenolic compounds and flavonoids in beverages and plant extracts using a CoulArray detector

2005 ◽  
Vol 28 (9-10) ◽  
pp. 1005-1022 ◽  
Author(s):  
Pavel Jandera ◽  
Veronika Škeříková ◽  
Lucie Řehová ◽  
Tomáš Hájek ◽  
Lucie Baldriánová ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Plant Extracts ◽  
Hplc Analysis ◽  
Rp Hplc

scholarly journals Stimulation of phenolic compounds production in the in vitro cultivated Polyscias filicifolia Bailey shoots and evaluation of the antioxidant and cytotoxic potential of plant extracts

2018 ◽  
Vol 87 (2) ◽  
Author(s):  
Anita Agnieszka Śliwińska ◽  
Katarzyna Sykłowska-Baranek ◽  
Anita Kośmider ◽  
Sebastian Granica ◽  
Karolina Miszczak ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Cytotoxic Activity ◽  
Phenolic Compound ◽  
Plant Extracts ◽  
Detrimental Effect ◽  
A549 Cells ◽  
Ferulic Acids

<p>In this study, an efficient method to enhance phenolic compound production in the in vitro cultured shoots of <em>Polyscias filicifolia</em> was developed. The phenolic compound content in <em>P. filicifolia</em> has not yet been reported. Shoots were treated with methyl jasmonate (JM) or salicylic acid (SA) at doses of 50, 100, or 200 µM. HPLC-UV-VIS and LC-MS techniques were used for the determination of chlorogenic, caffeic, and ferulic acids. The total phenolics and flavonoids were quantified, and the antioxidant capacity of plant extracts was determined using DPPH and ABTS methods. Finally, the cytotoxic activity of <em>P. filicifolia</em> extracts in normal (HaCaT) and cancer (A549) cells was investigated. Further, the effect of the extracts on cisplatin cytotoxicity was assessed.</p><p>The elicitors significantly enhanced phenolic production compared to that in untreated shoots and leaves of intact plants. Chlorogenic acid was the most abundant compound with the highest yield of 5.03 ±0.25 mg/g DW after treatment with 50 µM SA. The total flavonoid and phenolic content was significantly and dose-dependently influenced by JM. The highest antioxidant capacity was noted in extracts derived from shoots grown on media supplemented with 50 µM SA and 200 µM JM; these doses were used for further cytotoxic activity investigations. The extracts from JM or SA treatments reduced cancer cell viability and increased their mortality, whereas the extract from JM treatment exhibited protective effect on normal cells. Moreover, the comparison of cytotoxic properties of plant extracts and cisplatin indicated that plant phenolic compounds in combination with anticancer drugs could reduce the detrimental effect of the latter on human cells.</p>

Oxidative Stability of Edible Vegetable Oils Enriched in Polyphenols with Olive Leaf Extract

2007 ◽  
Vol 13 (6) ◽  
pp. 413-421 ◽  
Author(s):  
F.N. Salta ◽  
A. Mylona ◽  
A. Chiou ◽  
G. Boskou ◽  
N.K. Andrikopoulos
Keyword(s):  
Phenolic Compounds ◽  
Oxidative Stability ◽  
Antioxidant Capacity ◽  
Hplc Analysis ◽  
Leaf Extract ◽  
Olive Leaf ◽  
Total Polyphenols ◽  
Olive Leaf Extract ◽  
Rancimat Method ◽  
Oil Sunflower

Commercially available oils (olive oil, sunflower oil, palm oil, and a vegetable shortening) were enriched in polyphenols, by adding olive leaf extract. Addition of the extract was performed in such way that the oils were enriched with 200 mg/kg polyphenols. Total polyphenols of both enriched and commercial oils were estimated by the Folin—Ciocalteau assay, while identification and quantification of individual simple phenolic compounds was performed by GC/MS and of oleuropein by HPLC analysis. The enrichment resulted in the supplementation of the commercial oils mainly with oleuropein, hydroxytyrosol, and quercetin. Antioxidant capacity and oxidative stability of the enriched oils and the commercial ones were assessed by the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•) scavenging assay and the Rancimat method, respectively. Both antioxidant capacity and oxidative stability were substantially improved for all the oils studied after supplementation. By the procedure adopted, oils rich in polyphenols, mainly in oleuropein, can be produced with olive leaf extract supplementation.

scholarly journals Comparison of Techniques and Solvents on the Antimicrobial and Antioxidant Potential of Extracts from Acacia dealbata and Olea europaea

Antibiotics ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 48 ◽  
Author(s):  
Anabela Borges ◽  
Helena José ◽  
Vera Homem ◽  
Manuel Simões
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Capacity ◽  
Plant Extracts ◽  
Olea Europaea ◽  
Antioxidant Activities ◽  
Extraction Yield ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Acacia Dealbata ◽  
Olea Europaéa

Ethnopharmacological use of plant natural extracts has been known since ancient times. The optimization of plant molecule extraction is fundamental in obtaining relevant extraction yields. The main purpose of this study was to understand the role of different extraction techniques (solid-liquid, ultrasound, Soxhlet, and microwave) and solvents (water, methanol, ethanol, acetone, dichloromethane, and hexane) on the antimicrobial and antioxidant activities of extracts from Olea europaea (olive) and Acacia dealbata (mimosa). Crude plant extracts were evaluated for their antimicrobial activity against Staphylococcus aureus and Escherichia coli by the disk diffusion method. The antioxidant capacity of the extracts was determined by ABTS (2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid)) and DPPH (2,2-diphenyl-1-picrylhydrazyl) methods. In terms of extraction yield, ultrasound extraction and the solvents methanol, acetone (O. europaea) or water (A. dealbata) were found to be the best options. However, ethanol and acetone proved to be the best solvents to extract compounds with antimicrobial activity and antioxidant capacity, respectively (regardless of the extraction method employed). Soxhlet and microwave were the best techniques to extract compounds with antimicrobial activity, whereas any of the tested techniques showed the ability to extract compounds with antioxidant capacity. In most of the cases, both plant extracts (mimosa and olive) were more efficient against S. aureus than E. coli. In the present study, both mimosa and olive leaf crude extracts proved to have antimicrobial and antioxidant activities, increasing the demand of these natural products as a source of compounds with health benefits.

scholarly journals HPLC Analysis of Chemical Composition of Selected Jordanian Medicinal Plants and Their Bioactive Properties

2018 ◽  
Vol 34 (5) ◽  
pp. 2397-2403
Author(s):  
Fuad Al-Rimawi ◽  
Fadi Alakhras ◽  
Wael A. Al-Zereini ◽  
Hammad K. Aldal'in ◽  
Saleh Abu-Lafi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Hplc Analysis ◽  
Room Temperature ◽  
Bacterial Strains ◽  
E Coli ◽  
Bioactive Properties

Three medicinal plants grown wild in Jordan, namely Achillea santolina L, Achillea fragrantisimma, Asteriscus graveolens (Forssk) Less, were extracted with ethyl acetate by continuous shaking at room temperature for three days. The antibacterial activity of the crude extract was evaluated. The extracts were analyzed for their phenolic and flavonoids content by HPLC-PDA. The HPLC analysis of the plant extracts revealed the presence of flavonoids and phenolic compounds in the three plant extracts. Results revealed a strong antibacterial activity of A. graveolens against three bacterial strains (B. subtilis, E. coli, and S.aureus) while A. fragrantissima inhibited the growth of B. subtilis. Bioactivities were attributed mainly to the immense content of phenol-based compounds in plants.

scholarly journals Identification and Antibacterial Evaluation of Selected Jordanian Medicinal Plants

2018 ◽  
Vol 34 (5) ◽  
pp. 2456-2463
Author(s):  
Wael A. Al-Zereini ◽  
Fuad Al-Rimawi ◽  
Saleh Abu-Lafi ◽  
Fadi Alakhras ◽  
Ghassab M. Al-Mazaideh ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Plant Extracts ◽  
Crude Extract ◽  
Hplc Analysis ◽  
Room Temperature ◽  
Aerial Parts ◽  
Antibacterial Evaluation

Dried aerial parts of three medicinal plants grown wild in Jordan, namely Ononis natrix L., Salvia spinosa L. and Salvia verbenace L., were extracted upon soaking with ethyl acetate by continuous shaking at room temperature for three days. The extracts were analyzed for their phenolic and flavonoids content by HPLC-PDA. The HPLC analysis of the plant extracts revealed the presence of flavonoids and phenolic compounds in the three plant extracts. The antibacterial activity of the crude extract was evaluated. The PDA wavelengths range was from 227-347 nm. Bioactivities were attributed mainly to the immense content of phenol-based compounds in plants.

scholarly journals Phenolic compounds, antimicrobial activity, cytotoxicity, and identification of phytochemicals present in Inga marginata Willd seeds

2021 ◽  
Vol 43 ◽  
pp. e78
Author(s):  
Déborah Cristina Barcelos Flores ◽  
Caroline Pagnossim Boeira ◽  
Clarice Madalena Bueno Rolim ◽  
Daniele Rubert Nogueira Librelotto ◽  
Frederico Luiz Reis ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Cytotoxicity Assays ◽  
Antibacterial And Antifungal Activities ◽  
Low Concentrations ◽  
Phytochemical Compounds ◽  
Antibacterial And Antifungal

Brazil has an invaluable source of plants and fruits rich in phenolic compounds important to health, some of which have yet to be discovered. Inga marginata Willd is a fruit that can be found throughout Brazil, and its seeds are rich in phenolic compounds and antimicrobial activity, thus making their extraction and characterization highly relevant. This study aimed to determine the total phenolic compounds, antioxidant capacity, antibacterial and antifungal activities, cytotoxicity evaluation, and characterize the phytochemical compounds present in the extract. The results indicated that extraction by agitation at 30 ºC obtained the highest yield of total phenolic compounds (48.23 mg GAE g-1), antioxidant capacity (40.34 mg TEAC g-1), and IC50 (2.60 mg mL-1). The extracts at 30 and 60 ºC inhibited all microorganisms tested, and the temperature of 30 ºC acted as a bactericide and fungicide at low concentrations. Ten phytochemical compounds were found, mainly being antioxidants and antimicrobials. The cytotoxicity assays showed that Inga marginata seeds do not present cellular cytotoxicity up to the concentration of 250 µg mL-1, maintaining cell viability above 90%.

scholarly journals HPLC ANALYSIS OF PHENOLIC COMPOUNDS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF AMORPHA FRUTICOSA L. EXTRACTS

2019 ◽  
Vol 26 ◽  
pp. 77-84
Author(s):  
Bianca IVANESCU ◽  
Cristina LUNGU ◽  
Laurian VLASE ◽  
Adina Catinca GRADINARU ◽  
Cristina TUCHILUS
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Hplc Analysis ◽  
Amorpha Fruticosa ◽  
Antioxidant And Antimicrobial Activity
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