scholarly journals HPLC Analysis of Chemical Composition of Selected Jordanian Medicinal Plants and Their Bioactive Properties

2018 ◽  
Vol 34 (5) ◽  
pp. 2397-2403
Author(s):  
Fuad Al-Rimawi ◽  
Fadi Alakhras ◽  
Wael A. Al-Zereini ◽  
Hammad K. Aldal'in ◽  
Saleh Abu-Lafi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Hplc Analysis ◽  
Room Temperature ◽  
Bacterial Strains ◽  
E Coli ◽  
Bioactive Properties

Three medicinal plants grown wild in Jordan, namely Achillea santolina L, Achillea fragrantisimma, Asteriscus graveolens (Forssk) Less, were extracted with ethyl acetate by continuous shaking at room temperature for three days. The antibacterial activity of the crude extract was evaluated. The extracts were analyzed for their phenolic and flavonoids content by HPLC-PDA. The HPLC analysis of the plant extracts revealed the presence of flavonoids and phenolic compounds in the three plant extracts. Results revealed a strong antibacterial activity of A. graveolens against three bacterial strains (B. subtilis, E. coli, and S.aureus) while A. fragrantissima inhibited the growth of B. subtilis. Bioactivities were attributed mainly to the immense content of phenol-based compounds in plants.

scholarly journals Identification and Antibacterial Evaluation of Selected Jordanian Medicinal Plants

2018 ◽  
Vol 34 (5) ◽  
pp. 2456-2463
Author(s):  
Wael A. Al-Zereini ◽  
Fuad Al-Rimawi ◽  
Saleh Abu-Lafi ◽  
Fadi Alakhras ◽  
Ghassab M. Al-Mazaideh ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Plant Extracts ◽  
Crude Extract ◽  
Hplc Analysis ◽  
Room Temperature ◽  
Aerial Parts ◽  
Antibacterial Evaluation

Dried aerial parts of three medicinal plants grown wild in Jordan, namely Ononis natrix L., Salvia spinosa L. and Salvia verbenace L., were extracted upon soaking with ethyl acetate by continuous shaking at room temperature for three days. The extracts were analyzed for their phenolic and flavonoids content by HPLC-PDA. The HPLC analysis of the plant extracts revealed the presence of flavonoids and phenolic compounds in the three plant extracts. The antibacterial activity of the crude extract was evaluated. The PDA wavelengths range was from 227-347 nm. Bioactivities were attributed mainly to the immense content of phenol-based compounds in plants.

scholarly journals Chemical Composition and Antibacterial Activity of Berries Essential Oil of Algerian Juniperus thurifera (Var. aurasiaca)

2018 ◽  
Vol 24 (3) ◽  
pp. 240-245
Author(s):  
Lamia Boudjedjou ◽  
Messaoud Ramdani ◽  
Azzeddine Zeraib ◽  
Tarek Benmeddour ◽  
Azzedine Fercha
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Synergistic Effects ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Juniperus Thurifera ◽  
E Coli ◽  
Conventional Antibiotics ◽  
Promising Source

Background: Over the past decade, most antibiotic research programs have focused on finding new compounds with antimicrobial activity. This study aims to investigate the chemical composition and antibacterial activity of the essential oil (EO) extracted from ripe berries of Algerian Juniperus thurifera var. aurasiaca. Methods: The chemical composition of J. thurifera EO extracted by hydrodistillation was analyzed by using the GC-MS technique. Antibacterial activity of EO alone and in combination with three conventional antibiotics was assessed by using disc diffusion method against four bacterial strains. Results: Thirty-five components were identified, representing ~87 % of the oil. The main components were m-mentha-6,8-diene (15.43 %), β-pinene (10.59 %), elemol (8.31 %) and terpinene-4-ol (7.44 %). The essential oil showed strong antibacterial activity against S. aureus and E. coli, but no activity against P. aeruginosa and B. subtilis. Synergistic effects were observed because of the combined application of EO with gentamicin against all strains tested, and with amoxicillin against B. subtilis. Furthermore, the combination of EO/cefazolin demonstrated an additive effect against B. subtilis. In contrast, the combination of EO with amoxicillin and céfazoline revealed antagonistic effects against S. aureus, E. coli, and P. aeruginosa. Conclusion: This is the first report on the chemical composition and antibacterial activity of Algerian juniper berries’ essential oil. The results indicate that the studied EO may be a promising source of antibacterial compounds that could be useful for pharmaceutical applications especially in combination with conventional antibiotics.

scholarly journals Étude de la Sensibilité Aux Huiles Essentielles de Cinnamomum Verum, Eucalyptus Globulus, et Glycyrrhiza Glabra L Ainsi qu’aux Antibiotiques de Certains Germes Issus de la Restauration Collective

2018 ◽  
Vol 14 (3) ◽  
pp. 584
Author(s):  
Milan Oba Samoussa ◽  
Abderrazak Abdellaoui ◽  
Anass Kettani ◽  
Rachid Saile ◽  
Houda Bennani
Keyword(s):  
Staphylococcus Aureus ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Eucalyptus Globulus ◽  
Glycyrrhiza Glabra ◽  
Bacterial Strains ◽  
Salmonella Spp ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Glycyrrhiza Glabra L

Food safety is still a very important topic of interest. The use of medicinal plants extracts can be an efficient alternative for fighting food-borne infections in the face of the increase of resistance to antibiotics. We have studied the sensitivity of bacterial strains isolated from food outlets using commonly used antibiotics (Amoxicillin, Vancomycin, Ceftriaxone, Teicoplanin, Rifampicin and Amikacin). This was done using an antibiogram. We have also tested their sensitivity against essential oils extracted from medicinal plants (Cinnamomum verum, Eucalyptus globulus, and Glycyrrhiza glabra L) using aromatogram. This study was conducted using 27 bacterial strains, including 9 Escherichia coli strains, 9 Staphylococcus aureus strains, 9 Salmonella spp. strains, and 3 ATCC strains (E. coli ATCC 25922, Staphylococcus aureus ATCC 25923 et Salmonella typhimurium ATCC 14028). Results revealed that two plant extracts has a substantial antibacterial activity with zones of inhibition ranging from 10 to 25 mm, and it reached 35 mm when using a cocktail of plant extracts. Regarding the antibiotics we used, all strains of Salmonella spp. demonstrated a resistance to amoxicillin and to ceftriaxone. The tested strains of E. coli and Staphylococcus aureus had a partial resistance to the tested antibiotics, which confirms the results of previous studies.

scholarly journals ANIBACTERIAL EFFICACY AND GAS CHROMATOGRAPHY-MASS SPECTROMETERY ANALYSIS OF BIOACTIVE COMPOUNDS PRESENT IN DIFFERENT EXTRACTS OF ALLIUM SATIVUM

2018 ◽  
Vol 11 (4) ◽  
pp. 280
Author(s):  
Dushyant Sharma ◽  
Reena Rani ◽  
Monika Chaturvedi ◽  
Jaya Parkash Yadav
Keyword(s):  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Allium Sativum ◽  
Phytochemical Analysis ◽  
Dodecanoic Acid ◽  
Bacterial Strains ◽  
E Coli ◽  
Agar Well Diffusion Assay ◽  
Mass Spectrometery

 Objective: Medicinal plants are rich libraries containing wide variety of compounds of therapeutic values. Allium sativum commonly known as garlic is a very well-known medicinal plants being used with food products. In the present study, the antibacterial activity of different extracts of A. sativum was investigated along with their phytochemical analysis by gas chromatography-mass spectrometery (GC-MS) to explore antimicrobial compounds present in extracts.Methods: The antibacterial activity of A. sativum was evaluated against 9 reference bacterial strains and 3 MDR bacterial strains including Escherichia coli MDREC1, Klebsiella pneumoniae MDRKP2, and Pseudomonas aeruginosa MDRPA3 by microbroth dilution and agar well diffusion method.Results: The results obtained from agar well diffusion assay showed the zone of inhibition from 12 to 26 mm for different extracts. The methanol and acetone extracts were found most potent against reference and MDR bacterial strains. MIC values were in the range of 1.87–7.5 mg/ml. Further, GCMS analysis confirmed the presence of 35 compounds including dodecanoic acid, hexadecanoic acid, and methyl ester in common.Conclusion: The varied antimicrobial activity of extracts was due to the presence of different concentrations of the identified compounds which can be isolated and used for the treatment of various infectious diseases caused by MDR strains of E. coli, P. aeruginosa, and K. pneumoniae.

scholarly journals Synthesis, antibiotic structure–activity relationships, and cellulose dissolution studies of new room-temperature ionic liquids derived from lignin

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Shihong Liu ◽  
Michael Gonzalez ◽  
Celine Kong ◽  
Scott Weir ◽  
Aaron M. Socha
Keyword(s):  
Antibacterial Activity ◽  
Ionic Liquids ◽  
Room Temperature ◽  
Oxidation Products ◽  
Cellulose Dissolution ◽  
Melting Points ◽  
E Coli ◽  
Alkyl Chains ◽  
Renewable Feedstocks ◽  
Commodity Chemicals

Abstract Background Ionic liquids (ILs) are promising pretreatment solvents for lignocellulosic biomass, but are largely prepared from petroleum precursors. Benzaldehydes from depolymerized lignin, such as vanillin, syringaldehyde, and 4-methoxy benzaldehyde, represent renewable feedstocks for the synthesis of ionic liquids. We herein report syntheses of novel lignin-derived ionic liquids, with extended N-alkyl chains, and examine their melting points, cellulose dissolution capacities, and toxicity profiles against Daphnia magna and E. coli strain 1A1. The latter organism has been engineered to produce isoprenol, a drop-in biofuel and precursor for commodity chemicals. Results The new N,N-diethyl and N,N-dipropyl methyl benzylammonium ILs were liquids at room temperature, showing 75–100 °C decreased melting points as compared to their N,N,N-trimethyl benzylammonium analog. Extension of N-alkyl chains also increased antibacterial activity threefold, while ionic liquids prepared from vanillin showed 2- to 4-fold lower toxicity as compared to those prepared from syringaldehyde and 4-methoxybenzaldehyde. The trend of antibacterial activity for anions of lignin-derived ILs was found to be methanesulfonate < acetate < hydroxide. Microcrystalline cellulose dissolution, from 2 to 4 wt% after 20 min at 100 °C, was observed in all new ILs using light microscopy and IR spectroscopy. Conclusions Ionic liquids prepared from H-, S- and G-lignin oxidation products provided differential cytotoxic activity against E. coli and D. magna, suggesting these compounds could be tailored for application specificity within a biorefinery.

scholarly journals Chemical Composition and Antibacterial and Antioxidant Activity of a Citrus Essential Oil and Its Fractions

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2888
Author(s):  
Carmen M. S. Ambrosio ◽  
Gloria L. Diaz-Arenas ◽  
Leidy P. A. Agudelo ◽  
Elena Stashenko ◽  
Carmen J. Contreras-Castillo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Biological Activities ◽  
E Coli ◽  
Beneficial Bacterium ◽  
Minor Compounds ◽  
Antibacterial And Antioxidant Activity ◽  
High Relative Amount

Essential oils (EOs) from Citrus are the main by-product of Citrus-processing industries. In addition to food/beverage and cosmetic applications, citrus EOs could also potentially be used as an alternative to antibiotics in food-producing animals. A commercial citrus EO—Brazilian Orange Terpenes (BOT)—was fractionated by vacuum fractional distillation to separate BOT into various fractions: F1, F2, F3, and F4. Next, the chemical composition and biological activities of BOT and its fractions were characterized. Results showed the three first fractions had a high relative amount of limonene (≥10.86), even higher than the whole BOT. Conversely, F4 presented a larger relative amount of BOT’s minor compounds (carvone, cis-carveol, trans-carveol, cis-p-Mentha-2,8-dien-1-ol, and trans-p-Mentha-2,8-dien-1-ol) and a very low relative amount of limonene (0.08–0.13). Antibacterial activity results showed F4 was the only fraction exhibiting this activity, which was selective and higher activity on a pathogenic bacterium (E. coli) than on a beneficial bacterium (Lactobacillus sp.). However, F4 activity was lower than BOT. Similarly, F4 displayed the highest antioxidant activity among fractions (equivalent to BOT). These results indicated that probably those minor compounds that detected in F4 would be more involved in conferring the biological activities for this fraction and consequently for the whole BOT, instead of the major compound, limonene, playing this role exclusively.

scholarly journals Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv. Syrah grape pomace extract

2019 ◽  
Vol 10 (4) ◽  
pp. 1856-1869 ◽  
Author(s):  
Joana R. Costa ◽  
Manuela Amorim ◽  
Ana Vilas-Boas ◽  
Renata V. Tonon ◽  
Lourdes M. C. Cabral ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
Human Health ◽  
Grape Pomace ◽  
Vitis Vinifera L ◽  
Gastrointestinal Digestion ◽  
Gastrointestinal Health ◽  
Bioactive Properties

Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including in gastrointestinal health.

scholarly journals Alkaloid-Rich Crude Extracts, Fractions and Piperamide Alkaloids of Piper guineense Possess Promising Antibacterial Effects

Antibiotics ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 98 ◽  
Author(s):  
Eunice Mgbeahuruike ◽  
Pia Fyhrquist ◽  
Heikki Vuorela ◽  
Riitta Julkunen-Tiitto ◽  
Yvonne Holm
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Hot Water ◽  
Bacterial Strains ◽  
Inhibitory Effects ◽  
Piper Guineense ◽  
E Coli ◽  
Growth Inhibitory ◽  
Derivatives Of

Piper guineense is a food and medicinal plant commonly used to treat infectious diseases in West-African traditional medicine. In a bid to identify new antibacterial compounds due to bacterial resistance to antibiotics, twelve extracts of P. guineense fruits and leaves, obtained by sequential extraction, as well as the piperine and piperlongumine commercial compounds were evaluated for antibacterial activity against human pathogenic bacteria. HPLC-DAD and UHPLC/Q-TOF MS analysis were conducted to characterize and identify the compounds present in the extracts with promising antibacterial activity. The extracts, with the exception of the hot water decoctions and macerations, contained piperamide alkaloids as their main constituents. Piperine, dihydropiperine, piperylin, dihydropiperylin or piperlonguminine, dihydropiperlonguminine, wisanine, dihydrowisanine and derivatives of piperine and piperidine were identified in a hexane extract of the leaf. In addition, some new piperamide alkaloids were identified, such as a piperine and a piperidine alkaloid derivative and two unknown piperamide alkaloids. To the best of our knowledge, there are no piperamides reported in the literature with similar UVλ absorption maxima and masses. A piperamide alkaloid-rich hexane leaf extract recorded the lowest MIC of 19 µg/mL against Sarcina sp. and gave promising growth inhibitory effects against S. aureus and E. aerogenes as well, inhibiting the growth of both bacteria with a MIC of 78 µg/mL. Moreover, this is the first report of the antibacterial activity of P. guineense extracts against Sarcina sp. and E. aerogenes. Marked growth inhibition was also obtained for chloroform extracts of the leaves and fruits against P. aeruginosa with a MIC value of 78 µg/mL. Piperine and piperlongumine were active against E. aerogenes, S. aureus, E. coli, S. enterica, P. mirabilis and B. cereus with MIC values ranging from 39–1250 µg/mL. Notably, the water extracts, which were almost devoid of piperamide alkaloids, were not active against the bacterial strains. Our results demonstrate that P. guineense contains antibacterial alkaloids that could be relevant for the discovery of new natural antibiotics.

scholarly journals Investigating In Vitro Antibacterial Activities of Medicinal Plants Having Folkloric Repute in Ethiopian Traditional Medicine

2019 ◽  
Vol 24 ◽  
pp. 2515690X1988627 ◽  
Author(s):  
Mekonnen Sisay ◽  
Negussie Bussa ◽  
Tigist Gashaw ◽  
Getnet Mengistu
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Antimicrobial Agents ◽  
Bacterial Isolates ◽  
Zone Of Inhibition ◽  
Traditional Use ◽  
Gram Negative ◽  
E Coli ◽  
Slow Development

Medicinal plants are targeted in the search for new antimicrobial agents. Nowadays, there is an alarmingly increasing antimicrobial resistance to available agents with a very slow development of new antimicrobials. It is, therefore, necessary to extensively search for new agents based on the traditional use of herbal medicines as potential source. The antibacterial activity of 80% methanol extracts of the leaves of Verbena officinalis (Vo-80ME), Myrtus communis (Mc-80ME), and Melilotus elegans (Me-80ME) was tested against 6 bacterial isolates using agar well diffusion technique. In each extract, 3 concentrations of 10, 20, and 40 mg/well were tested for each bacterium. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also determined. Vo-80ME and Mc-80ME exhibited promising antibacterial activity against Staphylococcus aureus with the highest zone of inhibition being 18.67 and 26.16 mm, respectively at concentration of 40 mg/well. Regarding gram-negative bacteria, Vo-80ME exhibited an appreciable activity against Escherichia coli and Salmonella typhi. Mc-80ME displayed remarkable activity against all isolates including Pseudomonas aeruginosa with the maximum zone of inhibition being 22.83 mm. Me-80ME exhibited better antibacterial activity against E coli, but its secondary metabolites had little or no activity against other gram-negative isolates. The MIC values of Vo-80ME ranged from 0.16 to 4.00 mg/mL. The lowest MIC was observed in Mc-80ME, with the value being 0.032 mg/mL. Mc-80ME had bactericidal activity against all tested bacterial isolates. Mc-80ME showed remarkable zone of inhibitions in all tested bacterial isolates. Besides, Vo-80ME showed good antibacterial activity against S aureus, E coli, and S typhi. Conversely, Me-80ME has shown good activity against E coli only. Generally, M communis L and V officinalis have good MIC and MBC results.

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