scholarly journals Identification and Antibacterial Evaluation of Selected Jordanian Medicinal Plants

2018 ◽  
Vol 34 (5) ◽  
pp. 2456-2463
Author(s):  
Wael A. Al-Zereini ◽  
Fuad Al-Rimawi ◽  
Saleh Abu-Lafi ◽  
Fadi Alakhras ◽  
Ghassab M. Al-Mazaideh ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Plant Extracts ◽  
Crude Extract ◽  
Hplc Analysis ◽  
Room Temperature ◽  
Aerial Parts ◽  
Antibacterial Evaluation

Dried aerial parts of three medicinal plants grown wild in Jordan, namely Ononis natrix L., Salvia spinosa L. and Salvia verbenace L., were extracted upon soaking with ethyl acetate by continuous shaking at room temperature for three days. The extracts were analyzed for their phenolic and flavonoids content by HPLC-PDA. The HPLC analysis of the plant extracts revealed the presence of flavonoids and phenolic compounds in the three plant extracts. The antibacterial activity of the crude extract was evaluated. The PDA wavelengths range was from 227-347 nm. Bioactivities were attributed mainly to the immense content of phenol-based compounds in plants.

scholarly journals HPLC Analysis of Chemical Composition of Selected Jordanian Medicinal Plants and Their Bioactive Properties

2018 ◽  
Vol 34 (5) ◽  
pp. 2397-2403
Author(s):  
Fuad Al-Rimawi ◽  
Fadi Alakhras ◽  
Wael A. Al-Zereini ◽  
Hammad K. Aldal'in ◽  
Saleh Abu-Lafi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Hplc Analysis ◽  
Room Temperature ◽  
Bacterial Strains ◽  
E Coli ◽  
Bioactive Properties

Three medicinal plants grown wild in Jordan, namely Achillea santolina L, Achillea fragrantisimma, Asteriscus graveolens (Forssk) Less, were extracted with ethyl acetate by continuous shaking at room temperature for three days. The antibacterial activity of the crude extract was evaluated. The extracts were analyzed for their phenolic and flavonoids content by HPLC-PDA. The HPLC analysis of the plant extracts revealed the presence of flavonoids and phenolic compounds in the three plant extracts. Results revealed a strong antibacterial activity of A. graveolens against three bacterial strains (B. subtilis, E. coli, and S.aureus) while A. fragrantissima inhibited the growth of B. subtilis. Bioactivities were attributed mainly to the immense content of phenol-based compounds in plants.

scholarly journals Chemical Studies and Antibacterial Activity of the Root of Rumex abyssinicus

2020 ◽  
pp. 155-173
Author(s):  
Sisay Awoke ◽  
Chalachew Gedamu
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Chromatographic Separation ◽  
Pathogenic Bacteria ◽  
Endemic Plants ◽  
Chemical Studies ◽  
High Concentrations ◽  
Column Chromatographic ◽  
Antibacterial Evaluation

One of the Ethiopian endemic plants used traditionally as medicine for different diseases is Rumex abyssinicus. It is widely known as ‘Meqmeqo’ in Amharic. The aim of this study was to isolate and characterize compounds from the root of R. abyssinicus and to test their bioactivities of solvent fractions of the extract. The crude extract was extracted from dried powdered root of the plant with methanol. Then it was fractionated with n‑hexane and ethyl acetate with increasing polarity. The yellow powder obtained from ethyl acetate fractionation was applied to column chromatographic separation that led to the isolation of two compounds, diisobutyl phthalate and emodine. Finally, antibacterial evaluation of the ethyl acetate and methanol soluble portions were carried out against four pathogenic bacteria. Both methanol and ethyl acetate soluble portions inhibited all selected bacterial in better at high concentrations.

scholarly journals Biological Activities and Chemical Characterization ofCordia verbenaceaDC. as Tool to Validate the Ethnobiological Usage

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Edinardo Fagner Ferreira Matias ◽  
Erivânia Ferreira Alves ◽  
Beatriz Sousa Santos ◽  
Celestina Elba Sobral de Souza ◽  
João Victor de Alencar Ferreira ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Synergistic Effect ◽  
Crude Extract ◽  
Hplc Analysis ◽  
Antibacterial Effect ◽  
Biological Activities ◽  
Chemical Characterization ◽  
Analgesic Agents

Knowledge of medicinal plants is often the only therapeutic resource of many communities and ethnic groups. “Erva-baleeira”,Cordia verbenaceaDC., is one of the species of plants currently exploited for the purpose of producing a phytotherapeutic product extracted from its leaves. In Brazil, its major distribution is in the region of the Atlantic Forest and similar vegetation. The crude extract is utilized in popular cultures in the form of hydroalcoholic, decoctions and infusions, mainly as antimicrobial, antiinflammatory and analgesic agents. The aim of the present study was to establish a chemical and comparative profile of the experimental antibacterial activity and resistance modifying activity with ethnopharmacological reports. Phytochemical prospecting and HPLC analysis of the extract and fractions were in agreement with the literature with regard to the presence of secondary metabolites (tannins and flavonoids). The extract and fraction tested did not show clinically relevant antibacterial activity, but a synergistic effect was observed when combined with antibiotic, potentiating the antibacterial effect of aminoglycosides. We conclude that tests of antibacterial activity and modulating the resistance presented in this work results confirm the ethnobotanical and ethnopharmacological information, serving as a parameter in the search for new alternatives for the treatment of diseases.

scholarly journals Pharmacological Evaluation of Selected Medicinal Plants Used in the Management of Oral and Skin Infections in Ebem-Ohafia District, Abia State, Nigeria

2018 ◽  
Vol 2018 ◽  
pp. 1-16
Author(s):  
Blessing O. Oyedemi ◽  
Sunday O. Oyedemi ◽  
Johnson V. Chibuzor ◽  
Ifeoma I. Ijeh ◽  
Roger M. Coopoosamy ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Molecular Mechanisms ◽  
Current Trend ◽  
Radical Scavenging ◽  
Herbal Remedies ◽  
Dependent Manner ◽  
Skin Infections ◽  
Antioxidant Power

Oral and skin infections contribute significantly to the global health challenges responsible for the current trend of increased morbidity and premature death. The purpose of this study was to document medicinal plants used in the management of oral and skin infections in Ebem-Ohafia Local Government Area (LGA), Abia State, and to characterize the in vitro antioxidant and antibacterial activity. The thin layer chromatography (TLC) profiling of ten of the selected folklore medicine was carried out using a various solvent system of different polarity index. The antioxidant capacity of the plant extracts was evaluated using chemical-based methods, and its antibacterial effect was investigated using disc diffusion and microdilution methods. Sixty-one plant species belonging to 26 families were discovered, and the most frequently cited species are Euphorbiaceae (18.03%), Fabaceae (11.47%), and Asteraceae (11.47%). All the plant extracts showed a promising free radical scavenging activity and efficient ferric reducing antioxidant power in a concentration-dependent manner possibly due to their richness in polyphenol with TLC profiling showing maximum three bands of phytochemicals. Also, the plant extracts exhibited a mild to weak antibacterial activity against our panel of bacterial strains having MIC values ranging from 256 to > 512 μg/ mL reflected in their zone of inhibition at 10 μg/disc. The data obtained for Breynia nivosa (BN), Eleusine indica (EI), Cassia alata (CA), Chromolaena odorata (CO), and Acalypha hispida (AH) extracts substantiate the traditional use of these herbal remedies in the region and open the possibility for the development of cheaper and affordable drugs in the treatment of oral and skin infections. Further studies are needed to identify active ingredient with strong antibacterial and antioxidant capacities along with their molecular mechanisms.

Isolation of Quality Total RNA from the Aromatic Plant Origanum onites

2010 ◽  
Vol 65 (3-4) ◽  
pp. 266-270 ◽  
Author(s):  
Īsmail Poyraz ◽  
Emel Sözen ◽  
Muhittin Arslanyolu
Keyword(s):  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Reverse Transcription ◽  
Extraction Method ◽  
Room Temperature ◽  
Aromatic Plant ◽  
Guanidine Isothiocyanate ◽  
Total Rna ◽  
Root Tissues ◽  
Origanum Onites

We successfully used the guanidine isothiocyanate method for isolation of total RNA from leaf, stem, and root tissues of the aromatic plant Origanum onites. The RNA was extracted with TRI Reagent® at room temperature and was recovered by isopropanol precipitation. The isolated RNA was capable of reverse transcription. The extraction method described here does not require ultracentrifugation, and it is fast, simple, and effective. The procedure can be completed within 3 hours and may be applicable to other aromatic medicinal plants containing high amounts of phenolic compounds.

scholarly journals Antibacterial activity in the leaves of seven bitter medicinal plants of Bangladesh

1970 ◽  
Vol 18 ◽  
pp. 128-133 ◽  
Author(s):  
MTH Molla ◽  
MS Ahsan ◽  
MT Alam ◽  
ME Haque
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Ethanolic Extracts

Context: Development of resistance in human pathogens against conventional antibiotic necessitates searching indigenous medicinal plants having antibacterial property. Seven medicinal plants used actively in folklore, ayurvedic and traditional system of medicine were selected for the evaluation of their antimicrobial activity for this study.   Objectives: Evaluation of the effectiveness of some medicinal plant extracts against four Gram-positive and five Gram-negative bacteria.  Materials and Methods: The antibacterial activity of the crude ethanolic extracts obtained from the leaves of seven medicinal plants; viz., Andrographis paniculata, Catharanthus roseus, Adhatoda vasica, Vitex vegundo, Aloe vera, Flacortia ramontchi and Nyctanthes arbortristis were tested against nine bacteria at concentrations of 300-, 400- and 500 μg/ml. Standard antibiotic disc kanamycin (30μg/ml) was used for comparison. The minimum inhibitory concentration (MIC) of ethanolic extracts of the leaves of these medicinal plants were determined by testing the extracts on four Gram-positive and five Gram-negative bacteria by serial tube dilution method.   Results: All the extracts have notable antimicrobial activities against the test organisms. The ethanolic extracts of the leaves showed the highest antimicrobial activities against Bacillus megaterium and Shigella dysenteriae for An. paniculata, Ad. vasica and Al. vera; Bacillus subtilis and Salmonella typhi for C. roseus and N. arbortristis; Staphylociccus aureus and Salmonella typhi for V. vegundo; and Bacillus subtilis and Shigella sonnei for F. ramontchi respectively. The extract of the plants had MIC values ranging from 32 to 128 mg/ml. All plant extracts showed no MIC against Shigella shiga and against Sarcina lutea only C. roseus showed MIC 128 mg/ml.   Conclusion: The results revealed that the ethanolic extracts of the plants under present investigation have notable antimicrobial activities.   Keywords: medicinal plants; antimicrobial screening; MIC; bacteria. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8788 JBS 2010; 18(0): 128-133

scholarly journals Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes in Sophora flavescens Aiton

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Jeongyeo Lee ◽  
Jaeeun Jung ◽  
Seung-Hyun Son ◽  
Hyun-Bi Kim ◽  
Young-Hee Noh ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acid ◽  
Hplc Analysis ◽  
Developmental Stages ◽  
Biological Activities ◽  
Expression Patterns ◽  
Coumaric Acid ◽  
Sophora Flavescens ◽  
Catechin Hydrate ◽  
Aerial Parts

Sophorae Radix (Sophora flavescens Aiton) has long been used in traditional medicine in East Asia due to the various biological activities of its secondary metabolites. Endogenous contents of phenolic compounds (phenolic acid, flavonol, and isoflavone) and the main bioactive compounds of Sophorae Radix were analyzed based on the qualitative HPLC analysis and evaluated in different organs and at different developmental stages. In total, 11 compounds were detected, and the composition of the roots and aerial parts (leaves, stems, and flowers) was significantly different. trans-Cinnamic acid and p-coumaric acid were observed only in the aerial parts. Large amounts of rutin and maackiain were detected in the roots. Four phenolic acid compounds (benzoic acid, caffeic acid, ferulic acid, and chlorogenic acid) and four flavonol compounds (kaempferol, catechin hydrate, epicatechin, and rutin) were higher in aerial parts than in roots. To identify putative genes involved in phenolic compounds biosynthesis, a total of 41 transcripts were investigated. Expression patterns of these selected genes, as well as the multiple isoforms for the genes, varied by organ and developmental stage, implying that they are involved in the biosynthesis of various phenolic compounds both spatially and temporally.

scholarly journals Plant Extract Control of the Fungi Associated with Different Egyptian Wheat Cultivars Grains

2014 ◽  
Vol 54 (3) ◽  
pp. 231-237 ◽  
Author(s):  
Zakaria Awad Mohamed Baka
Keyword(s):  
Medicinal Plants ◽  
Plant Extract ◽  
Plant Extracts ◽  
Curvularia Lunata ◽  
Aqueous Extracts ◽  
Wheat Cultivars ◽  
Water Extracts ◽  
Aerial Parts ◽  
Root And Shoot Length ◽  
Antifungal Potential

Abstract Grain samples of 14 Egyptian wheat cultivars were tested for seed-borne fungi. The deep freezing method was used. Five seed-borne fungi viz., Aspergillus flavus, A. niger, Curvularia lunata, Fusarium moniliforme and Penicillium chrysogenum were isolated from the wheat cultivars viz., Bani Suef 4, Bani Suef 5, Gemmiza 7, Gemmiza 9, Gemmiza 10, Giza 168, Misr 1, Misr 2, Sakha 93, Sakha 94, Shandaweel 1, Sids 1, Sids 2 and Sids 3. A. flavus, A. niger and F. moniliforme were the most prevalent fungal species. Their incidence ranged from 21.0-53.5%, 16.0-37.5%, and 12.0-31.0%, respectively. The antifungal potential of water extracts from aerial parts of five wild medicinal plants (Asclepias sinaica, Farsetia aegyptia, Hypericum sinaicum, Phagnalon sinaicum, and Salvia aegyptiaca) were collected from the Sinai Peninsula, Egypt. The antifungal potential of water extracts from the aerial parts of these five plants were tested in the laboratory against the dominant fungi isolated from the wheat cultivars. All the aqueous plant extracts significantly (p ≤ 0.05) reduced the incidence of the tested seed-borne fungi. But the extract of Asclepias sinaica exhibited the most antifungal activity on tested fungi at all concentrations used when compared with other plant extracts. Maximum infested grain germination was observed in Giza 168 and minimum in Bani Suef 5. Treating grains with plant extract of A. sinaica (10%) enhanced the percentage of grain germination of all cultivars in both laboratory and pot experiments. Maximum root and shoot length of seedlings was recorded in Bani Suef 4 during fungal infestation or treatment by plant extract. For one hour before sowing or storage, the aqueous extract of A. sinaica can be used to treat wheat grains, to reduce the fungal incidence. Aqueous extracts of the aerial parts of selected medicinal plants, particularly A. sinaica, are promising for protecting Egyptian wheat grain cultivars against major seed-borne fungi. The aqueous extracts are expected to improve crops.

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