Reconstitution of a Highly Reducing Type II PKS System Reveals 6π-Electrocyclization Is Required for o-Dialkylbenzene Biosynthesis

2021 ◽  
Vol 143 (7) ◽  
pp. 2962-2969
Author(s):  
Jie Zhang ◽  
Satoshi Yuzawa ◽  
Wei Li Thong ◽  
Tetsuro Shinada ◽  
Makoto Nishiyama ◽  
...  
Keyword(s):  
Type Ii ◽  
Type Ii Pks

scholarly journals Genome mining and biosynthesis of kitacinnamycins as a STING activator

2019 ◽  
Vol 10 (18) ◽  
pp. 4839-4846 ◽  
Author(s):  
Jing Shi ◽  
Cheng Li Liu ◽  
Bo Zhang ◽  
Wen Jie Guo ◽  
Jiapeng Zhu ◽  
...  
Keyword(s):  
Genome Mining ◽  
Type Ii ◽  
Unique Type ◽  
Type Ii Pks

Genome mining targeting unique type II PKS and NRPS led to the identification of a novel class of glycopeptides named kitacinnamycins.

Cloning and Heterologous Expression of Three Type II PKS Gene Clusters fromStreptomyces bottropensis

ChemBioChem ◽  
2011 ◽  
Vol 13 (2) ◽  
pp. 224-230 ◽  
Author(s):  
Xiaohui Yan ◽  
Katharina Probst ◽  
Anton Linnenbrink ◽  
Moritz Arnold ◽  
Thomas Paululat ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Gene Clusters ◽  
Type Ii ◽  
Type Ii Pks

Post Polyketide Synthase Carbon–Carbon Bond Formation in Type-II PKS-Derived Natural Products fromStreptomyces venezuelae

2018 ◽  
Vol 83 (4) ◽  
pp. 1876-1890 ◽  
Author(s):  
Andrew W. Robertson ◽  
Jeanna M. MacLeod ◽  
Logan W. MacIntyre ◽  
Stephanie. M. Forget ◽  
Steven R. Hall ◽  
...  
Keyword(s):  
Natural Products ◽  
Polyketide Synthase ◽  
Bond Formation ◽  
Type Ii ◽  
Carbon Bond ◽  
Carbon Carbon Bond ◽  
Type Ii Pks

scholarly journals Ketide Synthase (KS) Domain Prediction and Analysis of Iterative Type II PKS Gene in Marine Sponge-Associated Actinobacteria Producing Biosurfactants and Antimicrobial Agents

2016 ◽  
Vol 7 ◽  
Author(s):  
Joseph Selvin ◽  
Ganesan Sathiyanarayanan ◽  
Anuj N. Lipton ◽  
Naif Abdullah Al-Dhabi ◽  
Mariadhas Valan Arasu ◽  
...  
Keyword(s):  
Marine Sponge ◽  
Antimicrobial Agents ◽  
Type Ii ◽  
Domain Prediction ◽  
Type Ii Pks

scholarly journals Genome-based analysis of the type II PKS biosynthesis pathway of xanthones in Streptomyces caelestis and their antifungal activity

RSC Advances ◽  
2019 ◽  
Vol 9 (64) ◽  
pp. 37376-37383
Author(s):  
Ling-Li Liu ◽  
Hong-Fei Liu ◽  
Hua-Hua Gao ◽  
Zheng-Zhong Yang ◽  
Xiao-Lan Feng ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi ◽  
Type Ii ◽  
Biosynthesis Pathway ◽  
Antifungal Activities ◽  
Liquid Fermentation ◽  
Type Ii Pks

The ethyl acetate extract from the liquid fermentation of S. caelestis Aw99c exhibited high and broad antifungal activities against plant pathogenic fungi.

scholarly journals Biogeography of Actinomycete Communities and Type II Polyketide Synthase Genes in Soils Collected in New Jersey and Central Asia

2007 ◽  
Vol 73 (9) ◽  
pp. 2982-2989 ◽  
Author(s):  
Boris Wawrik ◽  
Djumaniyaz Kutliev ◽  
Urinova A. Abdivasievna ◽  
Jerome J. Kukor ◽  
Gerben J. Zylstra ◽  
...  
Keyword(s):  
New Jersey ◽  
16S Rrna ◽  
Central Asia ◽  
Polyketide Synthase ◽  
Bacterial Species ◽  
Soil Microbial Communities ◽  
Polymorphism Analysis ◽  
Type Ii ◽  
Soil Microbial ◽  
Type Ii Pks

ABSTRACT Soil microbial communities are believed to be comprised of thousands of different bacterial species. One prevailing idea is that “everything is everywhere, and the environment selects,” implying that all types of bacteria are present in all environments where their growth requirements are met. We tested this hypothesis using actinomycete communities and type II polyketide synthase (PKS) genes found in soils collected from New Jersey and Uzbekistan (n = 91). Terminal restriction fragment length polymorphism analysis using actinomycete 16S rRNA and type II PKS genes was employed to determine community profiles. The terminal fragment frequencies in soil samples had a lognormal distribution, indicating that the majority of actinomycete phylotypes and PKS pathways are present infrequently in the environment. Less than 1% of peaks were detected in more than 50% of samples, and as many as 18% of the fragments were unique and detected in only one sample. Actinomycete 16S rRNA fingerprints clustered by country of origin, indicating that unique populations are present in North America and Central Asia. Sequence analysis of type II PKS gene fragments cloned from Uzbek soil revealed 35 novel sequence clades whose levels of identity to genes in the GenBank database ranged from 68 to 92%. The data indicate that actinomycetes are patchily distributed but that distinct populations are present in North American and Central Asia. These results have implications for microbial bioprospecting and indicate that the cosmopolitan actinomycete species and PKS pathways may account for only a small proportion of the total diversity in soil.

Identification of the common biosynthetic gene cluster for both antimicrobial streptoaminals and antifungal 5-alkyl-1,2,3,4-tetrahydroquinolines

2019 ◽  
Vol 17 (9) ◽  
pp. 2370-2378 ◽  
Author(s):  
Taro Ozaki ◽  
Ryosuke Sugiyama ◽  
Morito Shimomura ◽  
Shinichi Nishimura ◽  
Shumpei Asamizu ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Type Ii ◽  
Biosynthetic Gene ◽  
The Common ◽  
Type Ii Pks

The new subfamily of type II PKS gene cluster is responsible for biosynthesis of structurally distinct streptoaminals (STAMs) and 5-alkyl-1,2,3,4-tetrahydroquinolines (5aTHQs).

scholarly journals Enantioselective synthesis and stereochemical determination of the highly reduced polyketide ishigamide

2021 ◽  
Vol 85 (1) ◽  
pp. 148-153
Author(s):  
Mitsuki Seo ◽  
Danyao Du ◽  
Yohei Katsuyama ◽  
Ryo Katsuta ◽  
Arata Yajima ◽  
...  
Keyword(s):  
Wittig Reaction ◽  
Enantioselective Synthesis ◽  
Chiral Hplc ◽  
Type Ii ◽  
Stille Coupling ◽  
Acid Moiety ◽  
Vinyl Iodide ◽  
Type Ii Pks

Abstract Ishigamide was isolated as a metabolite of a recombinant strain of Streptomyces sp. MSC090213JE08 and its unsaturated fatty acid moiety has been confirmed in vitro to be synthesized by a type II PKS. Biosynthesis of such a highly reduced polyketide by a type II PKS is worthy of note. However, absolute configuration of ishigamide remained unknown. (R)-Ishigamide was synthesized enantioselectively employing Stille coupling and Wittig reaction between three units, vinyl iodide, stannyldienal, and Wittig salt. Stereochemistry of natural ishigamide was determined to be R by chiral HPLC analysis comparing with the synthesized standard.

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