scholarly journals Fluostatins Produced by the Heterologous Expression of a TAR Reassembled Environmental DNA Derived Type II PKS Gene Cluster

2010 ◽  
Vol 132 (34) ◽  
pp. 11902-11903 ◽  
Author(s):  
Zhiyang Feng ◽  
Jeff H. Kim ◽  
Sean F. Brady
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Environmental Dna ◽  
Type Ii ◽  
Type Ii Pks

Cloning and Heterologous Expression of Three Type II PKS Gene Clusters fromStreptomyces bottropensis

ChemBioChem ◽  
2011 ◽  
Vol 13 (2) ◽  
pp. 224-230 ◽  
Author(s):  
Xiaohui Yan ◽  
Katharina Probst ◽  
Anton Linnenbrink ◽  
Moritz Arnold ◽  
Thomas Paululat ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Gene Clusters ◽  
Type Ii ◽  
Type Ii Pks

Identification of the common biosynthetic gene cluster for both antimicrobial streptoaminals and antifungal 5-alkyl-1,2,3,4-tetrahydroquinolines

2019 ◽  
Vol 17 (9) ◽  
pp. 2370-2378 ◽  
Author(s):  
Taro Ozaki ◽  
Ryosuke Sugiyama ◽  
Morito Shimomura ◽  
Shinichi Nishimura ◽  
Shumpei Asamizu ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Type Ii ◽  
Biosynthetic Gene ◽  
The Common ◽  
Type Ii Pks

The new subfamily of type II PKS gene cluster is responsible for biosynthesis of structurally distinct streptoaminals (STAMs) and 5-alkyl-1,2,3,4-tetrahydroquinolines (5aTHQs).

Direct Cloning, Genetic Engineering, and Heterologous Expression of the Syringolin Biosynthetic Gene Cluster inE. colithrough Red/ET Recombineering

ChemBioChem ◽  
2012 ◽  
Vol 13 (13) ◽  
pp. 1946-1952 ◽  
Author(s):  
Xiaoying Bian ◽  
Fan Huang ◽  
Francis A. Stewart ◽  
Liqiu Xia ◽  
Youming Zhang ◽  
...  
Keyword(s):  
Genetic Engineering ◽  
Heterologous Expression ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Direct Cloning

Discovery of Polycyclic Macrolide Shuangdaolides by Heterologous Expression of a Cryptic trans-AT PKS Gene Cluster

2021 ◽  
Author(s):  
Yang Liu ◽  
Haibo Zhou ◽  
Qiyao Shen ◽  
Guangzhi Dai ◽  
Fu Yan ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster

Identification of the kinanthraquinone biosynthetic gene cluster by expression of an atypical response regulator

2021 ◽  
Vol 85 (3) ◽  
pp. 714-721
Author(s):  
Risa Takao ◽  
Katsuyuki Sakai ◽  
Hiroyuki Koshino ◽  
Hiroyuki Osada ◽  
Shunji Takahashi
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Secondary Metabolite ◽  
Response Regulator ◽  
Bac Library ◽  
Gene Clusters ◽  
Biosynthetic Gene Cluster ◽  
Gene Organization ◽  
Biosynthetic Gene ◽  
Streptomyces Sp

ABSTRACT Recent advances in genome sequencing have revealed a variety of secondary metabolite biosynthetic gene clusters in actinomycetes. Understanding the biosynthetic mechanism controlling secondary metabolite production is important for utilizing these gene clusters. In this study, we focused on the kinanthraquinone biosynthetic gene cluster, which has not been identified yet in Streptomyces sp. SN-593. Based on chemical structure, 5 type II polyketide synthase gene clusters were listed from the genome sequence of Streptomyces sp. SN-593. Among them, a candidate gene cluster was selected by comparing the gene organization with grincamycin, which is synthesized through an intermediate similar to kinanthraquinone. We initially utilized a BAC library for subcloning the kiq gene cluster, performed heterologous expression in Streptomyces lividans TK23, and identified the production of kinanthraquinone and kinanthraquinone B. We also found that heterologous expression of kiqA, which belongs to the DNA-binding response regulator OmpR family, dramatically enhanced the production of kinanthraquinones.

scholarly journals Mining of a streptothricin gene cluster from Streptomyces sp. TP-A0356 genome via heterologous expression

2013 ◽  
Vol 56 (7) ◽  
pp. 619-627 ◽  
Author(s):  
JinE Li ◽  
ZhengYan Guo ◽  
Wei Huang ◽  
XiangXi Meng ◽  
GuoMin Ai ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Streptomyces Sp

scholarly journals A Type II Protein Secretory Pathway Required for Levansucrase Secretion by Gluconacetobacter diazotrophicus

2004 ◽  
Vol 186 (15) ◽  
pp. 5031-5039 ◽  
Author(s):  
Juan G. Arrieta ◽  
Mailin Sotolongo ◽  
Carmen Menéndez ◽  
Dubiel Alfonso ◽  
Luis E. Trujillo ◽  
...  
Keyword(s):  
Codon Usage ◽  
Outer Membrane ◽  
Gene Cluster ◽  
Secretory Pathway ◽  
Cosmid Library ◽  
Polymorphism Analysis ◽  
Type Ii ◽  
Gluconacetobacter Diazotrophicus ◽  
Type Iv ◽  
Geographical Regions

ABSTRACT The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10) to utilize plant sucrose. LsdA, unlike other extracellular levansucrases from gram-negative bacteria, is transported to the periplasm by a signal-peptide-dependent pathway. We identified an unusually organized gene cluster encoding at least the components LsdG, -O, -E, -F, -H, -I, -J, -L, -M, -N, and -D of a type II secretory system required for LsdA translocation across the outer membrane. Another open reading frame, designated lsdX, is located between the operon promoter and lsdG, but it was not identified in BLASTX searches of the DDBJ/EMBL/GenBank databases. The lsdX, -G, and -O genes were isolated from a cosmid library of strain SRT4 by complementation of an ethyl methanesulfonate mutant unable to transport LsdA across the outer membrane. The downstream genes lsdE, -F, -H, -I, -J, -L, -M, -N, and -D were isolated through chromosomal walking. The high G+C content (64 to 74%) and the codon usage of the genes identified are consistent with the G+C content and codon usage of the standard G. diazotrophicus structural gene. Sequence analysis of the gene cluster indicated that a polycistronic transcript is synthesized. Targeted disruption of lsdG, lsdO, or lsdF blocked LsdA secretion, and the bacterium failed to grow on sucrose. Replacement of Cys162 by Gly at the C terminus of the pseudopilin LsdG abolished the protein functionality, suggesting that there is a relationship with type IV pilins. Restriction fragment length polymorphism analysis revealed conservation of the type II secretion operon downstream of the levansucrase-levanase (lsdA-lsdB) locus in 14 G. diazotrophicus strains representing 11 genotypes recovered from four different host plants in diverse geographical regions. To our knowledge, this is the first report of a type II pathway for protein secretion in the Acetobacteraceae.

Structural Diversification of Lyngbyatoxin A by Host-Dependent Heterologous Expression of thetleABCBiosynthetic Gene Cluster

ChemBioChem ◽  
2016 ◽  
Vol 17 (15) ◽  
pp. 1407-1411 ◽  
Author(s):  
Lihan Zhang ◽  
Shotaro Hoshino ◽  
Takayoshi Awakawa ◽  
Toshiyuki Wakimoto ◽  
Ikuro Abe
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Structural Diversification
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