scholarly journals Highly Sensitive Protein Detection Based on Lanthanide Chelates with Antenna Ligands Providing a Linear Range of Five Orders of Magnitude

2009 ◽  
Vol 81 (22) ◽  
pp. 9449-9453 ◽  
Author(s):  
Thole Zuchner ◽  
Frank Schumer ◽  
Renate Berger-Hoffmann ◽  
Katrin Müller ◽  
Mathias Lukas ◽  
...  
Keyword(s):  
Protein Detection ◽  
Linear Range ◽  
Lanthanide Chelates ◽  
Highly Sensitive

scholarly journals Mass Spectrometry versus Conventional Techniques of Protein Detection: Zika Virus NS3 Protease Activity towards Cellular Proteins

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3732
Author(s):  
Agnieszka Dabrowska ◽  
Aleksandra Milewska ◽  
Joanna Ner-Kluza ◽  
Piotr Suder ◽  
Krzysztof Pyrc
Keyword(s):  
Mass Spectrometry ◽  
Zika Virus ◽  
Viral Infections ◽  
Protein Detection ◽  
Extensive Discussion ◽  
Protein Targets ◽  
Highly Sensitive ◽  
Infected Cells ◽  
Ns3 Protease ◽  
To Receive

Mass spectrometry (MS) used in proteomic approaches is able to detect hundreds of proteins in a single assay. Although undeniable high analytical power of MS, data acquired sometimes lead to confusing results, especially during a search of very selective, unique interactions in complex biological matrices. Here, we would like to show an example of such confusing data, providing an extensive discussion on the observed phenomenon. Our investigations focus on the interaction between the Zika virus NS3 protease, which is essential for virus replication. This enzyme is known for helping to remodel the microenvironment of the infected cells. Several reports show that this protease can process cellular substrates and thereby modify cellular pathways that are important for the virus. Herein, we explored some of the targets of NS3, clearly shown by proteomic techniques, as processed during infection. Unfortunately, we could not confirm the biological relevance of protein targets for viral infections detected by MS. Thus, although mass spectrometry is highly sensitive and useful in many instances, also being able to show directions where cell/virus interaction occurs, we believe that deep recognition of their biological role is essential to receive complete insight into the investigated process.

scholarly journals Highly sensitive and multiplexed in situ protein profiling with cleavable fluorescent streptavidin

2019 ◽  
Author(s):  
Renjie Liao ◽  
Diego Mastroeni ◽  
Paul D. Coleman ◽  
Jia Guo
Keyword(s):  
Signal Amplification ◽  
Individual Cell ◽  
Protein Detection ◽  
Protein Profiling ◽  
Detection Sensitivity ◽  
Layer By Layer ◽  
Protein Targets ◽  
Highly Sensitive ◽  
Wide Range

AbstractThe ability to perform highly sensitive and multiplexed in situ protein analysis is crucial to advance our understanding of normal physiology and disease pathogenesis. To achieve this goal, here we develop an approach using cleavable biotin conjugated antibodies and cleavable fluorescent streptavidin (CFS). In this approach, protein targets are first recognized by the cleavable biotin labeled antibodies. Subsequently, CFS is applied to stain the protein targets. Though layer-by-layer signal amplification using cleavable biotin conjugated orthogonal antibodies and CSF, the protein detection sensitivity can be enhanced by at least 10 fold, compared with the existing methods. After imaging, the fluorophores and the biotins unbound to streptavidin are removed by chemical cleavage. The leftover streptavidin is blocked by biotin. Upon reiterative analysis cycles, a large number of different proteins with a wide range of expression levels can be unambiguously detected in individual cell in situ.

scholarly journals Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor

Biosensors ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 210
Author(s):  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Rafael A. Galiullin ◽  
Andrey F. Kozlov ◽  
Ivan D. Shumov ◽  
...  
Keyword(s):  
Protein Detection ◽  
Covalent Immobilization ◽  
Silicon On Insulator ◽  
Target Protein ◽  
Ca 125 ◽  
Minimum Concentration ◽  
Immobilized Antibodies ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Biospecific Interaction

The detection of CA 125 protein in a solution using a silicon-on-insulator (SOI)-nanowire biosensor with n-type chip has been experimentally demonstrated. The surface of nanowires was modified by covalent immobilization of antibodies against CA 125 in order to provide the biospecificity of the target protein detection. We have demonstrated that the biosensor signal, which results from the biospecific interaction between CA 125 and the covalently immobilized antibodies, increases with the increase in the protein concentration. At that, the minimum concentration, at which the target protein was detectable with the SOI-nanowire biosensor, amounted to 1.5 × 10−16 M.

scholarly journals A highly sensitive fluorescent light-up probe for real-time detection of the endogenous protein target and its antagonism in live cells

2015 ◽  
Vol 3 (29) ◽  
pp. 5933-5937 ◽  
Author(s):  
Junlong Geng ◽  
Walter L. Goh ◽  
Chongjing Zhang ◽  
David P. Lane ◽  
Bin Liu ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Real Time ◽  
Fluorescent Probe ◽  
Protein Detection ◽  
Live Cells ◽  
Fluorescent Light ◽  
Mdm2 Protein ◽  
Protein Target ◽  
Endogenous Protein ◽  
Highly Sensitive

A target-specific switchable fluorescent probe for cellular Mdm2 protein detection (off–on) and drug discovery applications (on–off) targeting the p53 pathway.

Multiplexed homogeneous digital immunoassay based on single-particle motion analysis

Lab on a Chip ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 2113-2121 ◽  
Author(s):  
Kenji Akama ◽  
Hiroyuki Noji
Keyword(s):  
Motion Analysis ◽  
Single Particle ◽  
Analytical Method ◽  
Particle Motion ◽  
Protein Detection ◽  
Biomarker Detection ◽  
Multiple Protein ◽  
Highly Sensitive ◽  
Simple Protocol ◽  
Single Particle Motion

Homogeneous digital immunoassay is a powerful analytical method for highly sensitive biomarker detection with a simple protocol. By using this method, we demonstrated the simultaneous multiple protein detection.

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