Highly sensitive evanescent wave combination tapered fiber optic fluorosensor for protein detection

2008 ◽  
Author(s):  
Vincent Nardone ◽  
Rakesh Kapoor
Keyword(s):  
Evanescent Wave ◽  
Fiber Optic ◽  
Protein Detection ◽  
Tapered Fiber ◽  
Highly Sensitive

scholarly journals Passive Fiber Optic Evanescent Wave Sensor for the Measurement of Refractive Index at Various Temperatures using a Tunable Light Source

2019 ◽  
Vol 8 (6S) ◽  
pp. 681-691
Keyword(s):  
Refractive Index ◽  
Light Source ◽  
Evanescent Wave ◽  
Fiber Optic ◽  
Fiber Optic Sensor ◽  
Depth Of Penetration ◽  
The Core ◽  
Passive Fiber ◽  
Highly Sensitive ◽  
Shaped Glass

The study of refractive index of liquids over a range of 10oC to 60oC shows very interesting results to design and develop a highly sensitive passive fiber optic sensor based on a U-shaped glass probe. The depth of penetration of light that escaping from the core of the fiber into the cladding plays a crucial role in the development of a highly sensitive fiber optic evanescent wave sensor. The depth of penetration of an optical fiber striped off its cladding is directly related to the wavelength of the light, the index of refraction of the surrounding medium, the angle of incidence of light, the bending radius and thickness of the U-shaped probe. In the design of the sensor a U-shaped glass probe is used to replace the core of the fiber in the region of sensing, the diameter of which is same as that of the cores of a pair of insensitive fibers which in-turn connected to a tunable light source and an optical detector. The sensor is highly reliable, robust and easy to configure using multimode PCS fibers and the source operating at the wavelengths of 630nm, 660nm, 820nm and 850nm.

Detection of Listeria Monocytogenes Using an Automated Fiber-Optic Biosensor: RAPTOR

2006 ◽  
Vol 321-323 ◽  
pp. 1168-1171 ◽  
Author(s):  
Gi Young Kim ◽  
Mark T. Morgan ◽  
Daniel Ess ◽  
Byoung Kwon Hahm ◽  
Aparna Kothapalli ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Evanescent Wave ◽  
Fiber Optic ◽  
Detection Method ◽  
Laser Light ◽  
Fiber Surface ◽  
Fluorescent Light ◽  
Tapered Fiber ◽  
Excitation Laser

Fiber-optic biosensor uses light transmittable tapered fiber to send excitation laser light and receive emitted fluorescent light. The fluorescent light excited by an evanescent wave generated by the laser is quantitatively related to biomolecules immobilized on the fiber surface [1]. An automated fiber-optic biosensor based detection method for Listeria monocytogenes was developed in this research. Detections of Listeria monocytogenes in hotdog sample were performed to evaluate the method. By using the detection method with automated fiber-optic biosensor, 5.4×107 cfu/ml of Listeria monocytogenes was able to detect.

scholarly journals Mass Spectrometry versus Conventional Techniques of Protein Detection: Zika Virus NS3 Protease Activity towards Cellular Proteins

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3732
Author(s):  
Agnieszka Dabrowska ◽  
Aleksandra Milewska ◽  
Joanna Ner-Kluza ◽  
Piotr Suder ◽  
Krzysztof Pyrc
Keyword(s):  
Mass Spectrometry ◽  
Zika Virus ◽  
Viral Infections ◽  
Protein Detection ◽  
Extensive Discussion ◽  
Protein Targets ◽  
Highly Sensitive ◽  
Infected Cells ◽  
Ns3 Protease ◽  
To Receive

Mass spectrometry (MS) used in proteomic approaches is able to detect hundreds of proteins in a single assay. Although undeniable high analytical power of MS, data acquired sometimes lead to confusing results, especially during a search of very selective, unique interactions in complex biological matrices. Here, we would like to show an example of such confusing data, providing an extensive discussion on the observed phenomenon. Our investigations focus on the interaction between the Zika virus NS3 protease, which is essential for virus replication. This enzyme is known for helping to remodel the microenvironment of the infected cells. Several reports show that this protease can process cellular substrates and thereby modify cellular pathways that are important for the virus. Herein, we explored some of the targets of NS3, clearly shown by proteomic techniques, as processed during infection. Unfortunately, we could not confirm the biological relevance of protein targets for viral infections detected by MS. Thus, although mass spectrometry is highly sensitive and useful in many instances, also being able to show directions where cell/virus interaction occurs, we believe that deep recognition of their biological role is essential to receive complete insight into the investigated process.

scholarly journals Experimental Study of a Real-Time and Highly Sensitive Fiber-Optic Porous Silicon Temperature Sensing Probe

2018 ◽  
Vol 18 (13) ◽  
pp. 5361-5367
Author(s):  
Raffaele Caroselli ◽  
David Martin Sanchez ◽  
Salvador Ponce-Alcantara ◽  
Francisco Prats Quilez ◽  
Luis Torrijos Moran ◽  
...  
Keyword(s):  
Experimental Study ◽  
Porous Silicon ◽  
Real Time ◽  
Fiber Optic ◽  
Temperature Sensing ◽  
Highly Sensitive

scholarly journals Evanescent Wave Absorption Based S-shaped Fiber-optic Biosensor for Immunosensing Applications

2016 ◽  
Vol 168 ◽  
pp. 117-120 ◽  
Author(s):  
S. Chauhan ◽  
N. Punjabi ◽  
D. Sharma ◽  
S. Mukherji
Keyword(s):  
Evanescent Wave ◽  
Fiber Optic ◽  
Wave Absorption

Label-Free Thrombin Detection Using a Tapered Fiber-Optic Interferometric Aptasensor

2019 ◽  
Vol 37 (11) ◽  
pp. 2756-2761 ◽  
Author(s):  
Dandan Sun ◽  
Li-Peng Sun ◽  
Tuan Guo ◽  
Bai-Ou Guan
Keyword(s):  
Fiber Optic ◽  
Label Free ◽  
Tapered Fiber
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