scholarly journals Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor

Biosensors ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 210
Author(s):  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Rafael A. Galiullin ◽  
Andrey F. Kozlov ◽  
Ivan D. Shumov ◽  
...  
Keyword(s):  
Protein Detection ◽  
Covalent Immobilization ◽  
Silicon On Insulator ◽  
Target Protein ◽  
Ca 125 ◽  
Minimum Concentration ◽  
Immobilized Antibodies ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Biospecific Interaction

The detection of CA 125 protein in a solution using a silicon-on-insulator (SOI)-nanowire biosensor with n-type chip has been experimentally demonstrated. The surface of nanowires was modified by covalent immobilization of antibodies against CA 125 in order to provide the biospecificity of the target protein detection. We have demonstrated that the biosensor signal, which results from the biospecific interaction between CA 125 and the covalently immobilized antibodies, increases with the increase in the protein concentration. At that, the minimum concentration, at which the target protein was detectable with the SOI-nanowire biosensor, amounted to 1.5 × 10−16 M.

scholarly journals Aptamer-Sensitized Nanoribbon Biosensor for Ovarian Cancer Marker Detection in Plasma

Chemosensors ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 222
Author(s):  
Yuri D. Ivanov ◽  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Rafael A. Galiullin ◽  
Andrey F. Kozlov ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Silicon On Insulator ◽  
Target Protein ◽  
Ca 125 ◽  
Buffer Solution ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cancer Marker Detection ◽  
Biospecific Interaction ◽  
Soi Substrates

The detection of CA 125 protein in buffer solution with a silicon-on-insulator (SOI)-based nanoribbon (NR) biosensor was experimentally demonstrated. In the biosensor, sensor chips, bearing an array of 12 nanoribbons (NRs) with n-type conductance, were employed. In the course of the analysis with the NR biosensor, the target protein was biospecifically captured onto the surface of the NRs, which was sensitized with covalently immobilized aptamers against CA 125. Atomic force microscopy (AFM) and mass spectrometry (MS) were employed in order to confirm the formation of the probe–target complexes on the NR surface. Via AFM and MS, the formation of aptamer–antigen complexes on the surface of SOI substrates with covalently immobilized aptamers against CA 125 was revealed, thus confirming the efficient immobilization of the aptamers onto the SOI surface. The biosensor signal, resulting from the biospecific interaction between CA 125 and the NR-immobilized aptamer probes, was shown to increase with an increase in the target protein concentration. The minimum detectable CA 125 concentration was as low as 1.5 × 10−17 M. Moreover, with the biosensor proposed herein, the detection of CA 125 in the plasma of ovarian cancer patients was demonstrated.

scholarly journals Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 147
Author(s):  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Vladimir P. Popov ◽  
Igor N. Kupriyanov ◽  
Rafael A. Galiullin ◽  
...  
Keyword(s):  
Production Quality ◽  
Autism Spectrum ◽  
Covalent Immobilization ◽  
Biological Macromolecules ◽  
Label Free ◽  
Raman Scattering Spectroscopy ◽  
Dna Oligonucleotides ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virtually defect-free, meaning they could be used for highly sensitive detection of biological macromolecules. The Si-NR chips were then used for the highly sensitive nanoelectronics detection of DNA oligonucleotides (oDNAs), which represent synthetic analogs of 106a-5p microRNA (miR-106a-5p), associated with the development of autism spectrum disorders in children. The specificity of the analysis was attained by the sensitization of the Si-NR chip sur-face by covalent immobilization of oDNA probes, whose nucleotide sequence was complementary to the known sequence of miR-106a-5p. The use of the Si-NR chip was demonstrated to al-low for the rapid label-free real-time detection of oDNA at ultra-low (~10−17 M) concentrations.

scholarly journals Ultrasensitive Detection of 2,4-Dinitrophenol Using Nanowire Biosensor

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yuri D. Ivanov ◽  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Rafael A. Galiullin ◽  
Andrey F. Kozlov ◽  
...  
Keyword(s):  
Field Effect Transistors ◽  
Silicon Nanowire ◽  
Silicon On Insulator ◽  
Amino Silane ◽  
Protective Layers ◽  
Highly Sensitive ◽  
Wide Range ◽  
Detectable Concentration ◽  
Surface Modification Techniques ◽  
Highly Sensitive Detection

The method for the detection of 2,4-dinitrophenol (DNP) in solution is proposed. This method employs the sensors based on silicon nanowire field-effect transistors with protective layers of high-k dielectrics, whose surface is functionalized with an amino silane. Direct highly sensitive detection of DNP has been demonstrated, and the lowest detectable concentration of DNP was determined to be 10−14 M. Silicon-on-insulator nanowire (SOI-NW) sensors can well be employed for the rapid detection of a wide range of toxic and explosive compounds by selection of sensor surface modification techniques.

scholarly journals Raman Spectroscopy-Based Quality Control of “Silicon-On-Insulator” Nanowire Chips for the Detection of Brain Cancer-Associated MicroRNA in Plasma

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1333
Author(s):  
Kristina A. Malsagova ◽  
Vladimir P. Popov ◽  
Igor N. Kupriyanov ◽  
Tatyana O. Pleshakova ◽  
Rafael A. Galiullin ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Brain Cancer ◽  
Covalent Immobilization ◽  
Experimental Series ◽  
Silicon On Insulator ◽  
Detection Sensitivity ◽  
Buffer Solution ◽  
Chip Surface ◽  
Synthetic Dna ◽  
Highly Sensitive Detection

Application of micro-Raman spectroscopy for the monitoring of quality of nanowire sensor chips fabrication has been demonstrated. Nanowire chips have been fabricated on the basis of «silicon-on-insulator» (SOI) structures (SOI-NW chips). The fabrication of SOI-NW chips was performed by optical litography with gas-phase etching. The so-fabricated SOI-NW chips are intended for highly sensitive detection of brain cancer biomarkers in humans. In our present study, two series of experiments have been conducted. In the first experimental series, detection of a synthetic DNA oligonucleotide (oDNA) analogue of brain cancer-associated microRNA miRNA-363 in purified buffer solution has been performed in order to demonstrate the high detection sensitivity. The second experimental series has been performed in order to reveal miRNA-363 itself in real human plasma samples. To provide detection biospecificity, the SOI-NW chip surface was modified by covalent immobilization of probe oligonucleotides (oDNA probes) complementary to the target biomolecules. Using the SOI-NW sensor chips proposed herein, the concentration detection limit of the target biomolecules at the level of 3.3 × 10−17 M has been demonstrated. Thus, the approach employing the SOI-NW chips proposed herein represents an attractive tool in biomedical practice, aimed at the early revelation of oncological diseases in humans.

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