scholarly journals A highly sensitive and selective viral protein detection method based on RNA oligonucleotide nanoparticle

2010 ◽  
pp. 323 ◽  
Author(s):  
Changhyun Roh
Keyword(s):  
Viral Protein ◽  
Detection Method ◽  
Protein Detection ◽  
Highly Sensitive

scholarly journals Mass Spectrometry versus Conventional Techniques of Protein Detection: Zika Virus NS3 Protease Activity towards Cellular Proteins

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3732
Author(s):  
Agnieszka Dabrowska ◽  
Aleksandra Milewska ◽  
Joanna Ner-Kluza ◽  
Piotr Suder ◽  
Krzysztof Pyrc
Keyword(s):  
Mass Spectrometry ◽  
Zika Virus ◽  
Viral Infections ◽  
Protein Detection ◽  
Extensive Discussion ◽  
Protein Targets ◽  
Highly Sensitive ◽  
Infected Cells ◽  
Ns3 Protease ◽  
To Receive

Mass spectrometry (MS) used in proteomic approaches is able to detect hundreds of proteins in a single assay. Although undeniable high analytical power of MS, data acquired sometimes lead to confusing results, especially during a search of very selective, unique interactions in complex biological matrices. Here, we would like to show an example of such confusing data, providing an extensive discussion on the observed phenomenon. Our investigations focus on the interaction between the Zika virus NS3 protease, which is essential for virus replication. This enzyme is known for helping to remodel the microenvironment of the infected cells. Several reports show that this protease can process cellular substrates and thereby modify cellular pathways that are important for the virus. Herein, we explored some of the targets of NS3, clearly shown by proteomic techniques, as processed during infection. Unfortunately, we could not confirm the biological relevance of protein targets for viral infections detected by MS. Thus, although mass spectrometry is highly sensitive and useful in many instances, also being able to show directions where cell/virus interaction occurs, we believe that deep recognition of their biological role is essential to receive complete insight into the investigated process.

Highly sensitive detection method of retinoblastoma genetic predisposition and biomarkers

2021 ◽  
Author(s):  
Jessica Le Gall ◽  
Catherine Dehainault ◽  
Camille Benoist ◽  
Alexandre Matet ◽  
Livia Lumbroso-Le Rouic ◽  
...  
Keyword(s):  
Genetic Predisposition ◽  
Detection Method ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

scholarly journals Aptamer Based Point of Care Diagnostic for the Detection of Food Allergens

2021 ◽  
Author(s):  
Sarah Stidham ◽  
Valerie Villareal ◽  
Vasant Chellappa ◽  
Lucas Yoder ◽  
Olivia Alley ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Detection Method ◽  
Point Of Care ◽  
Protein Detection ◽  
Peanut Protein ◽  
The Novel ◽  
Children And Families ◽  
Peanut Allergens ◽  
Consumer Device ◽  
Detection Technologies

Abstract Aptamers, due to their small size, strong target affinity, and ease of chemical modification, are ideally suited for molecular detection technologies. Here, we describe successful use of aptamer technology in a consumer device for the detection of peanut antigen in food. The novel aptamer-based protein detection method is robust across a wide variety of food matrices and sensitive to peanut protein at concentrations as low as 12.5 ppm (37.5 µg peanut protein in the sample). Integration of the assay into a sensitive, stable, and consumer friendly portable device will empower users to easily and quickly assess the presence of peanut allergens in foods before eating. With most food reactions occurring outside the home, the type of technology described here has significant potential to improve lives for children and families.

scholarly journals Highly sensitive and multiplexed in situ protein profiling with cleavable fluorescent streptavidin

2019 ◽  
Author(s):  
Renjie Liao ◽  
Diego Mastroeni ◽  
Paul D. Coleman ◽  
Jia Guo
Keyword(s):  
Signal Amplification ◽  
Individual Cell ◽  
Protein Detection ◽  
Protein Profiling ◽  
Detection Sensitivity ◽  
Layer By Layer ◽  
Protein Targets ◽  
Highly Sensitive ◽  
Wide Range

AbstractThe ability to perform highly sensitive and multiplexed in situ protein analysis is crucial to advance our understanding of normal physiology and disease pathogenesis. To achieve this goal, here we develop an approach using cleavable biotin conjugated antibodies and cleavable fluorescent streptavidin (CFS). In this approach, protein targets are first recognized by the cleavable biotin labeled antibodies. Subsequently, CFS is applied to stain the protein targets. Though layer-by-layer signal amplification using cleavable biotin conjugated orthogonal antibodies and CSF, the protein detection sensitivity can be enhanced by at least 10 fold, compared with the existing methods. After imaging, the fluorophores and the biotins unbound to streptavidin are removed by chemical cleavage. The leftover streptavidin is blocked by biotin. Upon reiterative analysis cycles, a large number of different proteins with a wide range of expression levels can be unambiguously detected in individual cell in situ.

scholarly journals A Simple and Highly Sensitive Colorimetric Detection Method for Gaseous Formaldehyde

2010 ◽  
Vol 132 (12) ◽  
pp. 4046-4047 ◽  
Author(s):  
Liang Feng ◽  
Christopher J. Musto ◽  
Kenneth S. Suslick
Keyword(s):  
Detection Method ◽  
Colorimetric Detection ◽  
Highly Sensitive ◽  
Gaseous Formaldehyde

scholarly journals Highly Sensitive Protein Detection Based on Lanthanide Chelates with Antenna Ligands Providing a Linear Range of Five Orders of Magnitude

2009 ◽  
Vol 81 (22) ◽  
pp. 9449-9453 ◽  
Author(s):  
Thole Zuchner ◽  
Frank Schumer ◽  
Renate Berger-Hoffmann ◽  
Katrin Müller ◽  
Mathias Lukas ◽  
...  
Keyword(s):  
Protein Detection ◽  
Linear Range ◽  
Lanthanide Chelates ◽  
Highly Sensitive

scholarly journals A convenient detection system consisting of efficient Au@PtRu nanozymes and alcohol oxidase for highly sensitive alcohol biosensing

2020 ◽  
Vol 2 (4) ◽  
pp. 1583-1589
Author(s):  
Feng Lv ◽  
Yuzhu Gong ◽  
Yingying Cao ◽  
Yaoyao Deng ◽  
Shufeng Liang ◽  
...  
Keyword(s):  
Detection Method ◽  
Detection System ◽  
Alcohol Oxidase ◽  
Highly Sensitive

A convenient alcohol detection method involving efficient Au@PtRu nanozymes and alcohol oxidase was developed.

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