scholarly journals Lumen Expansion Facilitates Epiblast-Primitive Endoderm Fate Specification during Mouse Blastocyst Formation

2019 ◽  
Vol 51 (6) ◽  
pp. 684-697.e4 ◽  
Author(s):  
Allyson Quinn Ryan ◽  
Chii Jou Chan ◽  
François Graner ◽  
Takashi Hiiragi
Keyword(s):  
Blastocyst Formation ◽  
Primitive Endoderm ◽  
Mouse Blastocyst ◽  
Fate Specification

scholarly journals Lumen expansion facilitates epiblast-primitive endoderm fate specification in the mouse blastocyst formation

2019 ◽  
Author(s):  
Allyson Quinn Ryan ◽  
Chii Jou Chan ◽  
François Graner ◽  
Takashi Hiiragi
Keyword(s):  
Cell Fate ◽  
Critical Role ◽  
Spatial Segregation ◽  
General Mechanism ◽  
Cell Fate Specification ◽  
Blastocyst Formation ◽  
Primitive Endoderm ◽  
Mouse Blastocyst ◽  
Fate Specification ◽  
Cell Fate Control

SummaryMouse blastocyst formation involves lumen formation and cell fate specification. While many studies have investigated how the blastocyst cell lineages are specified through genetics and signaling, studies into the potential role of the fluid lumen have yet to be conducted. We discover that blastocyst fluid emerges by secretion of cytoplasmic vesicles to intercellular space in addition to trans-epithelial flow. We observe that the beginning of epiblast and primitive endoderm spatial segregation directly follows lumen coalescence. Notably, we show that perturbing lumen expansion by pharmacological and biophysical means impair the specification and spatial segregation of primitive endoderm cells within the blastocyst. Combined, our results suggest that blastocyst lumen expansion plays a critical role in guiding cell fate specification and positioning. As epithelial tissues typically form lumina, lumen expansion may provide a general mechanism of cell fate control in many tissues.

Apoptosis during mouse blastocyst formation: evidence for a role of high levels of reactive oxygen species

2002 ◽  
Vol 78 ◽  
pp. S272-S273
Author(s):  
Navid Esfandiari ◽  
Ashok Agarwal ◽  
Rakesh K Sharma ◽  
Jeffery M Goldberg ◽  
David R Nelson ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Blastocyst Formation ◽  
Mouse Blastocyst

scholarly journals In Vivo Functional Analysis of Ezrin during Mouse Blastocyst Formation

2001 ◽  
Vol 233 (1) ◽  
pp. 161-173 ◽  
Author(s):  
Nicolas Dard ◽  
Sophie Louvet ◽  
Angélica Santa-Maria ◽  
Joël Aghion ◽  
Marianne Martin ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Blastocyst Formation ◽  
Mouse Blastocyst

SRC Expression and Activity Regulate Mouse Blastocyst Formation.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 234-234 ◽  
Author(s):  
Holly Giannatselis ◽  
Andrew J. Watson
Keyword(s):  
Blastocyst Formation ◽  
Mouse Blastocyst ◽  
Expression And Activity

scholarly journals FGF signal-dependent segregation of primitive endoderm and epiblast in the mouse blastocyst

Development ◽  
2010 ◽  
Vol 137 (5) ◽  
pp. 715-724 ◽  
Author(s):  
Y. Yamanaka ◽  
F. Lanner ◽  
J. Rossant
Keyword(s):  
Primitive Endoderm ◽  
Mouse Blastocyst

scholarly journals GATA6 Levels Modulate Primitive Endoderm Cell Fate Choice and Timing in the Mouse Blastocyst

2014 ◽  
Vol 29 (4) ◽  
pp. 454-467 ◽  
Author(s):  
Nadine Schrode ◽  
Néstor Saiz ◽  
Stefano Di Talia ◽  
Anna-Katerina Hadjantonakis
Keyword(s):  
Cell Fate ◽  
Primitive Endoderm ◽  
Mouse Blastocyst ◽  
Endoderm Cell
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