scholarly journals In Vivo Functional Analysis of Ezrin during Mouse Blastocyst Formation

2001 ◽  
Vol 233 (1) ◽  
pp. 161-173 ◽  
Author(s):  
Nicolas Dard ◽  
Sophie Louvet ◽  
Angélica Santa-Maria ◽  
Joël Aghion ◽  
Marianne Martin ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Blastocyst Formation ◽  
Mouse Blastocyst

scholarly journals Functional analysis of the integration host factor site of the σ54Pu promoter of Pseudomonas putida by in vivo UV imprinting

2011 ◽  
Vol 82 (3) ◽  
pp. 591-601 ◽  
Author(s):  
Marc Valls ◽  
Rafael Silva-Rocha ◽  
Ildefonso Cases ◽  
Amalia Muñoz ◽  
Víctor de Lorenzo
Keyword(s):  
Functional Analysis ◽  
Pseudomonas Putida ◽  
Integration Host Factor ◽  
Host Factor

Functional analysis of the SUMOylation pathway in Drosophila

2008 ◽  
Vol 36 (5) ◽  
pp. 868-873 ◽  
Author(s):  
Ana Talamillo ◽  
Jonatan Sánchez ◽  
Rosa Barrio
Keyword(s):  
Functional Analysis ◽  
Fine Tuning ◽  
Model Systems ◽  
Post Translational Modification ◽  
Reversible Process ◽  
Cellular Processes ◽  
Tuning Mechanism ◽  
Sumo Conjugation

SUMOylation, a reversible process used as a ‘fine-tuning’ mechanism to regulate the role of multiple proteins, is conserved throughout evolution. This post-translational modification affects several cellular processes by the modulation of subcellular localization, activity or stability of a variety of substrates. A growing number of proteins have been identified as targets for SUMOylation, although, for many of them, the role of SUMO conjugation on their function is unknown. The use of model systems might facilitate the study of SUMOylation implications in vivo. In the present paper, we have compiled what is known about SUMOylation in Drosophila melanogaster, where the use of genetics provides new insights on SUMOylation's biological roles.

Inhibition of EMP2 expression prevents mouse blastocyst attachment in vitro and implantation in vivo

2002 ◽  
Vol 78 ◽  
pp. S4
Author(s):  
Molina B Dayal ◽  
Madhuri Wadehra ◽  
Teri Ord ◽  
Jonathan Braun ◽  
Carmen J Williams
Keyword(s):  
Mouse Blastocyst

scholarly journals In vivo functional analysis reveals specific roles for the integrin-binding sites of talin

2011 ◽  
Vol 124 (11) ◽  
pp. 1844-1856 ◽  
Author(s):  
S. J. Ellis ◽  
M. Pines ◽  
M. J. Fairchild ◽  
G. Tanentzapf
Keyword(s):  
Functional Analysis ◽  
Binding Sites

scholarly journals Functional Analysis of Coordinated Cleavage in V(D)J Recombination

1998 ◽  
Vol 18 (8) ◽  
pp. 4679-4688 ◽  
Author(s):  
Deok Ryong Kim ◽  
Marjorie A. Oettinger
Keyword(s):  
Functional Analysis ◽  
Divalent Cation ◽  
Chromosomal Translocations ◽  
Cleavage Reaction ◽  
Chemically Modified ◽  
Double Stranded Dna ◽  
Cleavage Complex ◽  
Helical Turn

ABSTRACT V(D)J recombination in vivo requires a pair of signals with distinct spacer elements of 12 and 23 bp that separate conserved heptamer and nonamer motifs. Cleavage in vitro by the RAG1 and RAG2 proteins can occur at individual signals when the reaction buffer contains Mn2+, but cleavage is restricted to substrates containing two signals when Mg2+ is the divalent cation. By using a novel V(D)J cleavage substrate, we show that while the RAG proteins alone establish a moderate preference for a 12/23 pair versus a 12/12 pair, a much stricter dependence of cleavage on the 12/23 signal pair is produced by the inclusion of HMG1 and competitor double-stranded DNA. The competitor DNA serves to inhibit the cleavage of substrates carrying a 12/12 or 23/23 pair, as well as the cutting at individual signals in 12/23 substrates. We show that a 23/33 pair is more efficiently recombined than a 12/33 pair, suggesting that the 12/23 rule can be generalized to a requirement for spacers that differ from each other by a single helical turn. Furthermore, we suggest that a fixed spatial orientation of signals is required for cleavage. In general, the same signal variants that can be cleaved singly can function under conditions in which a signal pair is required. However, a chemically modified substrate with one noncleavable signal enables us to show that formation of a functional cleavage complex is mechanistically separable from the cleavage reaction itself and that although cleavage requires a pair of signals, cutting does not have to occur simultaneously at both. The implications of these results are discussed with respect to the mechanism of V(D)J recombination and the generation of chromosomal translocations.

scholarly journals Functional analysis of Toxoplasma lactate dehydrogenases suggests critical roles of lactate fermentation for parasite growth in vivo

2017 ◽  
Vol 20 (1) ◽  
pp. e12794 ◽  
Author(s):  
Ningbo Xia ◽  
Jichao Yang ◽  
Shu Ye ◽  
Lihong Zhang ◽  
Yanqin Zhou ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Parasite Growth ◽  
Lactate Fermentation ◽  
Lactate Dehydrogenases
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