scholarly journals Lumen expansion facilitates epiblast-primitive endoderm fate specification in the mouse blastocyst formation

2019 ◽  
Author(s):  
Allyson Quinn Ryan ◽  
Chii Jou Chan ◽  
François Graner ◽  
Takashi Hiiragi
Keyword(s):  
Cell Fate ◽  
Critical Role ◽  
Spatial Segregation ◽  
General Mechanism ◽  
Cell Fate Specification ◽  
Blastocyst Formation ◽  
Primitive Endoderm ◽  
Mouse Blastocyst ◽  
Fate Specification ◽  
Cell Fate Control

SummaryMouse blastocyst formation involves lumen formation and cell fate specification. While many studies have investigated how the blastocyst cell lineages are specified through genetics and signaling, studies into the potential role of the fluid lumen have yet to be conducted. We discover that blastocyst fluid emerges by secretion of cytoplasmic vesicles to intercellular space in addition to trans-epithelial flow. We observe that the beginning of epiblast and primitive endoderm spatial segregation directly follows lumen coalescence. Notably, we show that perturbing lumen expansion by pharmacological and biophysical means impair the specification and spatial segregation of primitive endoderm cells within the blastocyst. Combined, our results suggest that blastocyst lumen expansion plays a critical role in guiding cell fate specification and positioning. As epithelial tissues typically form lumina, lumen expansion may provide a general mechanism of cell fate control in many tissues.

scholarly journals Lumen Expansion Facilitates Epiblast-Primitive Endoderm Fate Specification during Mouse Blastocyst Formation

2019 ◽  
Vol 51 (6) ◽  
pp. 684-697.e4 ◽  
Author(s):  
Allyson Quinn Ryan ◽  
Chii Jou Chan ◽  
François Graner ◽  
Takashi Hiiragi
Keyword(s):  
Blastocyst Formation ◽  
Primitive Endoderm ◽  
Mouse Blastocyst ◽  
Fate Specification

scholarly journals β-catenin has an ancestral role in cell fate specification but not cell adhesion

2019 ◽  
Author(s):  
Miguel Salinas-Saavedra ◽  
Athula H. Wikramanayake ◽  
Mark Q Martindale
Keyword(s):  
Cell Adhesion ◽  
Cell Fate ◽  
Target Genes ◽  
Critical Role ◽  
Mnemiopsis Leidyi ◽  
Epithelial Tissue ◽  
Cell Fate Specification ◽  
Animal Cells ◽  
Fate Specification

AbstractThe ß-catenin protein has two major known functions in animal cells. It keeps epithelial tissue homeostasis by its connection with Adherens Junctions (AJ), and it serves as a transcriptional cofactor along with Lef/Tcf to enter the nucleus and regulate target genes of the Wnt/ß-catenin (cWnt) signaling pathway. To assess the ancestral role of ß-catenin during development we examined its distribution and function in the ctenophore Mnemiopsis leidyi (one of the earliest branching animal phyla) by using ctenophore-specific antibodies and mRNA injection. We found that ß-catenin protein never localizes to cell-cell contacts during embryogenesis as it does in other metazoans, most likely because ctenophore-cadherins do not have the cytoplasmic domain required for interaction with the catenin proteins. Downregulation of zygotic Mlß-catenin signaling led to the loss of endodermal and mesodermal tissues indicating that nuclear ß-catenin may have a deep role in germ-layer evolution. Our results indicate that the ancestral role for ß-catenin was in the cell-fate specification and not in cell adhesion and also further emphasizes the critical role of this protein in the evolution of tissue layers in metazoans.

Sox proteins: regulators of cell fate specification and differentiation

Development ◽  
2013 ◽  
Vol 140 (20) ◽  
pp. 4129-4144 ◽  
Author(s):  
Y. Kamachi ◽  
H. Kondoh
Keyword(s):  
Cell Fate ◽  
Cell Fate Specification ◽  
Fate Specification ◽  
Sox Proteins

scholarly journals Differential requirement for Gli2 and Gli3 in ventral neural cell fate specification

2003 ◽  
Vol 259 (1) ◽  
pp. 150-161 ◽  
Author(s):  
Jun Motoyama ◽  
Ljiljana Milenkovic ◽  
Mizuho Iwama ◽  
Yayoi Shikata ◽  
Matthew P. Scott ◽  
...  
Keyword(s):  
Cell Fate ◽  
Neural Cell ◽  
Cell Fate Specification ◽  
Fate Specification ◽  
Neural Cell Fate
Sign in / Sign up

Export Citation Format

Share Document