scholarly journals GATA6 Levels Modulate Primitive Endoderm Cell Fate Choice and Timing in the Mouse Blastocyst

2014 ◽  
Vol 29 (4) ◽  
pp. 454-467 ◽  
Author(s):  
Nadine Schrode ◽  
Néstor Saiz ◽  
Stefano Di Talia ◽  
Anna-Katerina Hadjantonakis
Keyword(s):  
Cell Fate ◽  
Primitive Endoderm ◽  
Mouse Blastocyst ◽  
Endoderm Cell

scholarly journals PDGF signaling is required for primitive endoderm cell survival in the inner cell mass of the mouse blastocyst

Stem Cells ◽  
2013 ◽  
Vol 31 (9) ◽  
pp. 1932-1941 ◽  
Author(s):  
Jérôme Artus ◽  
Minjung Kang ◽  
Michel Cohen-Tannoudji ◽  
Anna-Katerina Hadjantonakis
Keyword(s):  
Cell Survival ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Primitive Endoderm ◽  
Mouse Blastocyst ◽  
Endoderm Cell ◽  
Pdgf Signaling

scholarly journals Lumen expansion facilitates epiblast-primitive endoderm fate specification in the mouse blastocyst formation

2019 ◽  
Author(s):  
Allyson Quinn Ryan ◽  
Chii Jou Chan ◽  
François Graner ◽  
Takashi Hiiragi
Keyword(s):  
Cell Fate ◽  
Critical Role ◽  
Spatial Segregation ◽  
General Mechanism ◽  
Cell Fate Specification ◽  
Blastocyst Formation ◽  
Primitive Endoderm ◽  
Mouse Blastocyst ◽  
Fate Specification ◽  
Cell Fate Control

SummaryMouse blastocyst formation involves lumen formation and cell fate specification. While many studies have investigated how the blastocyst cell lineages are specified through genetics and signaling, studies into the potential role of the fluid lumen have yet to be conducted. We discover that blastocyst fluid emerges by secretion of cytoplasmic vesicles to intercellular space in addition to trans-epithelial flow. We observe that the beginning of epiblast and primitive endoderm spatial segregation directly follows lumen coalescence. Notably, we show that perturbing lumen expansion by pharmacological and biophysical means impair the specification and spatial segregation of primitive endoderm cells within the blastocyst. Combined, our results suggest that blastocyst lumen expansion plays a critical role in guiding cell fate specification and positioning. As epithelial tissues typically form lumina, lumen expansion may provide a general mechanism of cell fate control in many tissues.

scholarly journals Culture of Mouse Amniotic Fluid-Derived Cells on Irradiated STO Feeders Results in the Generation of Primitive Endoderm Cell Lines Capable of Self-Renewal in vitro

2013 ◽  
Vol 198 (2) ◽  
pp. 111-126 ◽  
Author(s):  
Aleksandar M. Babic ◽  
Sunyoung Jang ◽  
Eugenia Nicolov ◽  
Horatiu Voicu ◽  
Chance J. Luckey
Keyword(s):  
Amniotic Fluid ◽  
Cell Lines ◽  
Primitive Endoderm ◽  
Self Renewal ◽  
Endoderm Cell

scholarly journals FGF signal-dependent segregation of primitive endoderm and epiblast in the mouse blastocyst

Development ◽  
2010 ◽  
Vol 137 (5) ◽  
pp. 715-724 ◽  
Author(s):  
Y. Yamanaka ◽  
F. Lanner ◽  
J. Rossant
Keyword(s):  
Primitive Endoderm ◽  
Mouse Blastocyst

Indian hedgehog activates hematopoiesis and vasculogenesis and can respecify prospective neurectodermal cell fate in the mouse embryo

Development ◽  
2001 ◽  
Vol 128 (10) ◽  
pp. 1717-1730 ◽  
Author(s):  
M.A. Dyer ◽  
S.M. Farrington ◽  
D. Mohn ◽  
J.R. Munday ◽  
M.H. Baron
Keyword(s):  
Endothelial Cells ◽  
Cell Fate ◽  
Mouse Embryo ◽  
Hedgehog Signaling ◽  
Vascular System ◽  
Vascular Endothelial Cells ◽  
Indian Hedgehog ◽  
Vascular Endothelial ◽  
Primitive Endoderm ◽  
Genes Encoding

During gastrulation in the mouse, mesoderm is induced and patterned by secreted signaling molecules, giving rise first to primitive erythroblasts and vascular endothelial cells. We have demonstrated previously that development of these lineages requires a signal(s) secreted from the adjacent primitive endoderm. We now show that Indian hedgehog (Ihh) is a primitive endoderm-secreted signal that alone is sufficient to induce formation of hematopoietic and endothelial cells. Strikingly, as seen with primitive endoderm, Ihh can respecify prospective neural ectoderm (anterior epiblast) along hematopoietic and endothelial (posterior) lineages. Downstream targets of the hedgehog signaling pathway (the genes encoding patched, smoothened and Gli1) are upregulated in anterior epiblasts cultured in the presence of Ihh protein, as is Bmp4, which may mediate the effects of Ihh. Blocking Ihh function in primitive endoderm inhibits activation of hematopoiesis and vasculogenesis in the adjacent epiblast, suggesting that Ihh is an endogenous signal that plays a key role in the development of the earliest hemato-vascular system. To our knowledge, these are the earliest functions for a hedgehog protein in post-implantation development in the mouse embryo.

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