Fluorescence enhancement and multiple protein detection in ZnO nanostructure microfluidic devices

2016 ◽  
Vol 75 ◽  
pp. 285-292 ◽  
Author(s):  
Chen-Hsiang Sang ◽  
Shu-Jen Chou ◽  
F.M. Pan ◽  
Jeng-Tzong Sheu
Keyword(s):  
Fluorescence Enhancement ◽  
Protein Detection ◽  
Microfluidic Devices ◽  
Zno Nanostructure ◽  
Multiple Protein

A simple and rapid method for blood plasma separation driven by capillary force with an application in protein detection

2020 ◽  
Vol 12 (20) ◽  
pp. 2560-2570 ◽  
Author(s):  
Qingxue Gao ◽  
Yongjia Chang ◽  
Qingmei Deng ◽  
Hui You
Keyword(s):  
Blood Plasma ◽  
Rapid Method ◽  
Capillary Force ◽  
Protein Detection ◽  
Microfluidic Devices ◽  
Treatment Procedure ◽  
Plasma Separation ◽  
Pre Treatment ◽  
Blood Plasma Separation

Blood plasma separation is a vital sample pre-treatment procedure for microfluidic devices in blood diagnostics, and it requires reliability and speediness.

scholarly journals 6-Aza-2-Thio-Thymine Stabilized Gold Nanoclusters as Photoluminescent Probe for Protein Detection

Nanomaterials ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 281 ◽  
Author(s):  
Hao-Hua Deng ◽  
Xiao-Qiong Shi ◽  
Paramasivam Balasubramanian ◽  
Kai-Yuan Huang ◽  
Ying-Ying Xu ◽  
...  
Keyword(s):  
Efficient Method ◽  
Fluorescence Enhancement ◽  
Gold Nanoclusters ◽  
Protein Detection ◽  
Real Samples ◽  
Cell Extracts ◽  
Photoluminescence Enhancement ◽  
In Virtue Of ◽  
Enhancement Property ◽  
Good Agreement

This study puts forward an efficient method for protein detection in virtue of the tremendous fluorescence enhancement property of 6-aza-2-thio-thymine protected gold nanoclusters (ATT-AuNCs). In-depth studies of the protein-induced photoluminescence enhancement mechanism illustrate the mechanism of the interaction between ATT-AuNCs and protein. This new-established probe enables feasible and sensitive quantification of the concentrations of total protein in real samples, such as human serum, human plasma, milk, and cell extracts. The results of this proposed method are in good agreement with those determined by the classical bicinchoninic acid method (BCA method).

Multiplexed homogeneous digital immunoassay based on single-particle motion analysis

Lab on a Chip ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 2113-2121 ◽  
Author(s):  
Kenji Akama ◽  
Hiroyuki Noji
Keyword(s):  
Motion Analysis ◽  
Single Particle ◽  
Analytical Method ◽  
Particle Motion ◽  
Protein Detection ◽  
Biomarker Detection ◽  
Multiple Protein ◽  
Highly Sensitive ◽  
Simple Protocol ◽  
Single Particle Motion

Homogeneous digital immunoassay is a powerful analytical method for highly sensitive biomarker detection with a simple protocol. By using this method, we demonstrated the simultaneous multiple protein detection.

Plasmonic micro-beads for fluorescence enhanced, multiplexed protein detection with flow cytometry

2014 ◽  
Vol 5 (10) ◽  
pp. 4070-4075 ◽  
Author(s):  
Bo Zhang ◽  
Jiang Yang ◽  
Yingping Zou ◽  
Ming Gong ◽  
Hui Chen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Small Molecule ◽  
Fluorescence Enhancement ◽  
Protein Detection ◽  
Protein Biomarkers ◽  
Abundant Protein

The fluorescence enhancement of small molecule fluorophores was achieved on micro-beads through gold nano-island coating, enabling the detection of low-abundant protein biomarkers.

On-chip graphene oxide aptasensor for multiple protein detection

2015 ◽  
Vol 866 ◽  
pp. 1-9 ◽  
Author(s):  
Yuko Ueno ◽  
Kazuaki Furukawa ◽  
Kota Matsuo ◽  
Suzuyo Inoue ◽  
Katsuyoshi Hayashi ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Protein Detection ◽  
Multiple Protein ◽  
On Chip

A smartphone readable colorimetric sensing platform for rapid multiple protein detection

The Analyst ◽  
2017 ◽  
Vol 142 (17) ◽  
pp. 3177-3182 ◽  
Author(s):  
Feiyang Wang ◽  
Yuexiang Lu ◽  
Jiacheng Yang ◽  
Ying Chen ◽  
Wenjie Jing ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Sensor Array ◽  
Point Of Care ◽  
Protein Detection ◽  
Colorimetric Sensor ◽  
Colorimetric Sensing ◽  
Colorimetric Sensor Array ◽  
Sensing Platform ◽  
Multiple Protein

We have developed a very simple colorimetric sensor array by using only unmodified gold nanoparticles and NaCl salt for discrimination of multiple proteins. The inexpensive and convenient sensor array and the ubiquitous smartphone are coupled to achieve an immediate point-of-care diagnosis without additional devices.

scholarly journals Single cell manipulation, analytics, and label-free protein detection in microfluidic devices for systems nanobiology

2005 ◽  
Vol 26 (19) ◽  
pp. 3689-3696 ◽  
Author(s):  
Wibke Hellmich ◽  
Christoph Pelargus ◽  
Kai Leffhalm ◽  
Alexandra Ros ◽  
Dario Anselmetti
Keyword(s):  
Single Cell ◽  
Protein Detection ◽  
Microfluidic Devices ◽  
Cell Manipulation ◽  
Label Free ◽  
Free Protein ◽  
Single Cell Manipulation

Fabrication of a Microfluidic Device for the Detection of a Specific Biomolecule

2008 ◽  
Vol 57 ◽  
pp. 105-110
Author(s):  
Won Jin Jeon ◽  
Chee Burm Shin ◽  
Jong Heop Yi
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Superoxide Dismutase ◽  
Microfluidic Devices ◽  
Biomolecular Recognition ◽  
Protein Antigens ◽  
Molding Method ◽  
Multiple Protein ◽  
Spr Imaging ◽  
Lateral Sclerosis

Mutation and aggregation of superoxide dismutase (SOD) are reported as one of the causes of amyotrophic lateral sclerosis (ALS). To detect SOD1 protein from the motor neuron, surface plasmon resonance (SPR) analysis was adopted due to its advantages in the in-situ biomolecular recognition by surface analysis without labeling. For the patterning of protein antigens at Au surface for use in SPR imaging experiments, microfluidic devices were fabricated with polydimethylsiloxane (PDMS) by replica molding method. They were designed for the solution to flow by capillary force only without using any additional pumping equipments or flow controllers. Performance of microfluidic devices was verified by the simple microfluidic experiments, and multiple protein-patterned sensor surfaces were constructed by using these microfluidic devices.

An electrochemical molecular recognition-based aptasensor for multiple protein detection

2015 ◽  
Vol 491 ◽  
pp. 31-36 ◽  
Author(s):  
Lin Cheng ◽  
Jie Zhang ◽  
Yan Lin ◽  
Qiong Wang ◽  
XiuXiu Zhang ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Protein Detection ◽  
Multiple Protein
Sign in / Sign up

Export Citation Format

Share Document