An electrochemical molecular recognition-based aptasensor for multiple protein detection

2015 ◽  
Vol 491 ◽  
pp. 31-36 ◽  
Author(s):  
Lin Cheng ◽  
Jie Zhang ◽  
Yan Lin ◽  
Qiong Wang ◽  
XiuXiu Zhang ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Protein Detection ◽  
Multiple Protein

Multiplexed homogeneous digital immunoassay based on single-particle motion analysis

Lab on a Chip ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 2113-2121 ◽  
Author(s):  
Kenji Akama ◽  
Hiroyuki Noji
Keyword(s):  
Motion Analysis ◽  
Single Particle ◽  
Analytical Method ◽  
Particle Motion ◽  
Protein Detection ◽  
Biomarker Detection ◽  
Multiple Protein ◽  
Highly Sensitive ◽  
Simple Protocol ◽  
Single Particle Motion

Homogeneous digital immunoassay is a powerful analytical method for highly sensitive biomarker detection with a simple protocol. By using this method, we demonstrated the simultaneous multiple protein detection.

scholarly journals pH-Triggered Reversible Multiple Protein–Polymer Conjugation Based on Molecular Recognition

2015 ◽  
Vol 119 (36) ◽  
pp. 12066-12073 ◽  
Author(s):  
Juan Liu ◽  
Viktoriia Postupalenko ◽  
Jason T. Duskey ◽  
Cornelia G. Palivan ◽  
Wolfgang Meier
Keyword(s):  
Molecular Recognition ◽  
Protein Polymer ◽  
Multiple Protein ◽  
Polymer Conjugation

On-chip graphene oxide aptasensor for multiple protein detection

2015 ◽  
Vol 866 ◽  
pp. 1-9 ◽  
Author(s):  
Yuko Ueno ◽  
Kazuaki Furukawa ◽  
Kota Matsuo ◽  
Suzuyo Inoue ◽  
Katsuyoshi Hayashi ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Protein Detection ◽  
Multiple Protein ◽  
On Chip

A smartphone readable colorimetric sensing platform for rapid multiple protein detection

The Analyst ◽  
2017 ◽  
Vol 142 (17) ◽  
pp. 3177-3182 ◽  
Author(s):  
Feiyang Wang ◽  
Yuexiang Lu ◽  
Jiacheng Yang ◽  
Ying Chen ◽  
Wenjie Jing ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Sensor Array ◽  
Point Of Care ◽  
Protein Detection ◽  
Colorimetric Sensor ◽  
Colorimetric Sensing ◽  
Colorimetric Sensor Array ◽  
Sensing Platform ◽  
Multiple Protein

We have developed a very simple colorimetric sensor array by using only unmodified gold nanoparticles and NaCl salt for discrimination of multiple proteins. The inexpensive and convenient sensor array and the ubiquitous smartphone are coupled to achieve an immediate point-of-care diagnosis without additional devices.

scholarly journals Analysis of multiple protein detection methods in human osteoporotic bone extracellular matrix: From literature to practice

Bone ◽  
2020 ◽  
Vol 137 ◽  
pp. 115363
Author(s):  
Caterina Licini ◽  
Giorgia Montalbano ◽  
Gabriela Ciapetti ◽  
Giorgia Cerqueni ◽  
Chiara Vitale-Brovarone ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Protein Detection ◽  
Detection Methods ◽  
Osteoporotic Bone ◽  
Multiple Protein

Aptamer-based silver nanosensor for multiple protein detection

Lab on a Chip ◽  
2012 ◽  
Vol 12 (17) ◽  
pp. 3184 ◽  
Author(s):  
Ying Wang ◽  
Danke Xu ◽  
Hong-Yuan Chen
Keyword(s):  
Protein Detection ◽  
Multiple Protein

scholarly journals Simultaneous Detection of High-Sensitivity Cardiac Troponin I and Myoglobin by Modified Sandwich Lateral Flow Immunoassay: Proof of Principle

2011 ◽  
Vol 57 (12) ◽  
pp. 1732-1738 ◽  
Author(s):  
Jimin Zhu ◽  
Nengli Zou ◽  
Danian Zhu ◽  
Jin Wang ◽  
Qinghui Jin ◽  
...  
Keyword(s):  
Detection Limit ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Simultaneous Detection ◽  
Protein Detection ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Cardiac Troponin I ◽  
Multiple Protein ◽  
Proof Of Principle

BACKGROUNDAlthough numerous lateral flow immunoassays (LFIAs) have been developed and widely used, inadequate analytical sensitivity and the lack of multiple protein detection applications have limited their clinical utility. We developed a new LFIA device for the simultaneous detection of high-sensitivity cardiac troponin I (hs-cTnI) and myoglobin (Myo).METHODSWe used a gold nanoparticle (AuNP) doubly labeled complex, in which biotinylated single-stranded DNA was used as a linkage to integrate 2 AuNPs and streptavidin-labeled AuNP, as an amplifier to magnify extremely low signals.RESULTSThe detection limit of 1 ng/L achieved for hs-cTnI was 1000 times lower than that obtained in a conventional LFIA. The detection limit for simultaneously measured Myo was 1 μg/L. The linear measurement ranges for hs-cTnI and Myo were 1–10 000 ng/L and 1–10 000 μg/L, respectively. We observed concordant results between the LFIA and clinical assays in sera from 12 patients with acute myocardial infarction (hs-cTnI r = 0.96; Myo r = 0.98). Assay imprecision was <11% for both hs-TnI and myo.CONCLUSIONSThe described proof-of-principle LFIA method could be used as a point-of-care device in multiple protein quantification and semiquantitative analysis.

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