scholarly journals Fluorescence enhancement of a bleach-resistant solution for use in microfluidic devices

2018 ◽  
Vol 8 (3) ◽  
pp. 676 ◽  
Author(s):  
Mitsunori Saito ◽  
Takahiro Koketsu
Keyword(s):  
Fluorescence Enhancement ◽  
Microfluidic Devices

Review of Microfluidic Devices for On-Chip Chemical Sensing

2016 ◽  
Vol 136 (6) ◽  
pp. 244-249
Author(s):  
Takahiro Watanabe ◽  
Fumihiro Sassa ◽  
Yoshitaka Yoshizumi ◽  
Hiroaki Suzuki
Keyword(s):  
Chemical Sensing ◽  
Microfluidic Devices ◽  
On Chip

A Photoactivable Formaldehyde Donor with Fluorescence Monitoring Reveals Threshold to Arrest Cell Migration

2019 ◽  
Author(s):  
Lukas P Smaga ◽  
Nicholas W Pino ◽  
Gabriela E Ibarra ◽  
Vishnu Krishnamurthy ◽  
Jefferson Chan
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Fluorescence Enhancement ◽  
Biological Effects ◽  
Cellular Systems ◽  
Live Cells ◽  
First Report ◽  
Cell Lysate ◽  
Intracellular Release ◽  
Biological Analytes

Controlled light-mediated delivery of biological analytes enables the investigation of highly reactivity molecules within cellular systems. As many biological effects are concentration dependent, it is critical to determine the location, time, and quantity of analyte donation. In this work, we have developed the first photoactivatable donor for formaldehyde (FA). Our optimized photoactivatable donor, photoFAD-3, is equipped with a fluorescence readout that enables monitoring of FA release with a concomitant 139-fold fluorescence enhancement. Tuning of photostability and cellular retention enabled quantification of intracellular FA release through cell lysate calibration. Application of photoFAD-3 uncovered the concentration range necessary for arresting wound healing in live cells. This marks the first report where a photoactivatable donor for any analyte has been used to quantify intracellular release.

The Difference in Sensing Properties Towards Anions between Two Pyridinium Amide-Based Receptors with a Subtle Change of H-Bond Position

2020 ◽  
Vol 17 (6) ◽  
pp. 472-478
Author(s):  
Wei-tao Gong ◽  
Wei-dong Qu ◽  
Guiling Ning
Keyword(s):  
Fluorescence Enhancement ◽  
Small Difference ◽  
Fluorescence Emission ◽  
Subtle Change ◽  
Sensing Properties ◽  
Naked Eye ◽  
Recognition Ability ◽  
The Difference ◽  
L1 And L2

Two pyridinium amide-based receptors L1 and L2 with a small difference of H-bond position of the amide have been synthesized and characterized. Interestingly, they exhibited a huge difference in sensing towards AcO- and H2PO4 -, respectively. Receptor L1 was found to be ‘naked-eye’ selective for AcO- anions, while receptor L2 showed clear fluorescence enhancement selective to H2PO4 - anion. The recognition ability has been established by fluorescence emission, UV-vis spectra, and 1HNMR titration.

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