Molecular analyses of thyroid tumors for diagnosis of malignancy on fine-needle aspiration biopsies and for prognosis of invasiveness on surgical specimens

2011 ◽  
Vol 72 (2) ◽  
pp. 125-128 ◽  
Author(s):  
B. Rousset ◽  
L. Ziercher ◽  
F. Borson-Chazot
Keyword(s):  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Thyroid Tumors ◽  
Molecular Analyses ◽  
Fine Needle

A Rapid Method for DNA Extraction from Fine-Needle Aspiration Biopsies of Thyroid Tumors, and Subsequent RET Mutation Analysis

1998 ◽  
Vol 22 (6) ◽  
pp. 544-548 ◽  
Author(s):  
Jan Zedenius ◽  
Trisha Dwight ◽  
Bruce G. Robinson ◽  
Leigh Delbridge ◽  
Martin Backdahl ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Rapid Method ◽  
Mutation Analysis ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Thyroid Tumors ◽  
Fine Needle ◽  
Ret Mutation

scholarly journals Molecular analyses of prostate tumors for diagnosis of malignancy on fine-needle aspiration biopsies

Oncotarget ◽  
2017 ◽  
Vol 8 (62) ◽  
pp. 104761-104771 ◽  
Author(s):  
Menglin Shan ◽  
Qianlin Xia ◽  
Dong Yan ◽  
Yanjun Zhu ◽  
Xuan Zhang ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Prostate Tumors ◽  
Molecular Analyses ◽  
Fine Needle

scholarly journals Combined quantitation of HMGA2 mRNA, microRNAs, and mitochondrial-DNA content enables the identification and typing of thyroid tumors in fine-needle aspiration smears

BMC Cancer ◽  
2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Sergei E. Titov ◽  
Mikhail K. Ivanov ◽  
Pavel S. Demenkov ◽  
Gevork A. Katanyan ◽  
Eugenia S. Kozorezova ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Molecular Markers ◽  
Fine Needle Aspiration ◽  
Real Time Pcr ◽  
Thyroid Nodules ◽  
Nuclear Dna ◽  
Malignant Tumors ◽  
Needle Aspiration ◽  
Thyroid Tumors ◽  
Fine Needle

Abstract Background Analysis of molecular markers in addition to cytological analysis of fine-needle aspiration (FNA) samples is a promising way to improve the preoperative diagnosis of thyroid nodules. Nonetheless, in clinical practice, applications of existing diagnostic solutions based on the detection of somatic mutations or analysis of gene expression are limited by their high cost and difficulties with clinical interpretation. The aim of our work was to develop an algorithm for the differential diagnosis of thyroid nodules on the basis of a small set of molecular markers analyzed by real-time PCR. Methods A total of 494 preoperative FNA samples of thyroid goiters and tumors from 232 patients with known histological reports were analyzed: goiter, 105 samples (50 patients); follicular adenoma, 101 (48); follicular carcinoma, 43 (28); Hürthle cell carcinoma, 25 (11); papillary carcinoma, 121 (56); follicular variant of papillary carcinoma, 80 (32); and medullary carcinoma, 19 (12). Total nucleic acids extracted from dried FNA smears were analyzed for five somatic point mutations and two translocations typical of thyroid tumors as well as for relative concentrations of HMGA2 mRNA and 13 microRNAs and the ratio of mitochondrial to nuclear DNA by real-time PCR. A decision tree–based algorithm was built to discriminate benign and malignant tumors and to type the thyroid cancer. Leave-p-out cross-validation with five partitions was performed to estimate prediction quality. A comparison of two independent samples by quantitative traits was carried out via the Mann–Whitney U test. Results A minimum set of markers was selected (levels of HMGA2 mRNA and miR-375, − 221, and -146b in combination with the mitochondrial-to-nuclear DNA ratio) and yielded highly accurate discrimination (sensitivity = 0.97; positive predictive value = 0.98) between goiters with benign tumors and malignant tumors and accurate typing of papillary, medullary, and Hürthle cell carcinomas. The results support an alternative classification of follicular tumors, which differs from the histological one. Conclusions The study shows the feasibility of the preoperative differential diagnosis of thyroid nodules using a panel of several molecular markers by a simple PCR-based method. Combining markers of different types increases the accuracy of classification.

scholarly journals Vanishing Thyroid Tumors: A Diagnostic Dilemma After Ultrasonography-Guided Fine-Needle Aspiration

Thyroid ◽  
2013 ◽  
Vol 23 (2) ◽  
pp. 194-200 ◽  
Author(s):  
Ogechukwu P. Eze ◽  
Guoping Cai ◽  
Zubair W. Baloch ◽  
Ashraf Khan ◽  
Renu Virk ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Thyroid Tumors ◽  
Diagnostic Dilemma ◽  
Fine Needle

Fine-needle aspiration of thyroid tumors: Identifying factors associated with adequacy rate in a large academic center in the Netherlands

2010 ◽  
Vol 40 (S1) ◽  
pp. E21-E26 ◽  
Author(s):  
Sijbrigje G. A. de Meer ◽  
Jennifer M. J. Schreinemakers ◽  
Pierre M. J. Zelissen ◽  
Gerard Stapper ◽  
Daisy M. D. S. Sie-Go ◽  
...  
Keyword(s):  
The Netherlands ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Thyroid Tumors ◽  
Fine Needle ◽  
Factors Associated ◽  
Academic Center

Fine Needle Aspiration Biopsy for Thyroid Tumors. Treatment of Suspicious (Class III) Cases.

2001 ◽  
Vol 52 (1) ◽  
pp. 24-27 ◽  
Author(s):  
Ichiro Tateya ◽  
Ryo Asato ◽  
Kazuhiko Shoji ◽  
Masato Inoue ◽  
Shigeru Hirano ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Biopsy ◽  
Needle Aspiration ◽  
Aspiration Biopsy ◽  
Thyroid Tumors ◽  
Class Iii ◽  
Fine Needle ◽  
Needle Aspiration Biopsy

scholarly journals Diagnosis of Hodgkin Lymphoma from Cell Block: A Reliable and Helpful Tool in “Selected” Diagnostic Practice

Diagnostics ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 748
Author(s):  
Paola Parente ◽  
Claudia Covelli ◽  
Magda Zanelli ◽  
Domenico Trombetta ◽  
Illuminato Carosi ◽  
...  
Keyword(s):  
Hodgkin Lymphoma ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Cell Block ◽  
Ultrasound Guided ◽  
Block Method ◽  
Molecular Analyses ◽  
Fine Needle ◽  
Conventional Cytology ◽  
Morphological Evaluation

Background: The diagnosis of lymphoma requires surgical specimens to perform morphological evaluation, immunohistochemical and molecular analyses. Ultrasound-guided fine needle aspiration may represent an appropriate first approach to obtain cytological samples in impalpable lesions and/or in patients unsuitable for surgical procedures. Although cytology has intrinsic limitations, the cell block method may increase the possibility of achieving an accurate diagnosis. Methods: We retrospectively selected a total of 47 ultrasound-guided fine needle aspiration and drainage samples taken from patients with effusion and deep-seated lesions which are clinically suspicious in terms of malignancy. Results: In 27 cases, both cell block and conventional cytology were performed: 21/27 cell blocks were adequate for the diagnosis of lymphoma and suitable for immunocytochemistry and molecular analyses vs. 12/20 samples to which only conventional cytology was applied. Moreover, in five patients we were able to make a diagnosis of Hodgkin lymphoma with the cell block (CB) technique. Conclusions: Contrary to conventional cytology, the cell block method may allow immunocytochemistry and molecular studies providing useful information for the diagnosis and subtypization of lymphoma in patients unsuitable for surgical procedure or with deep-seated lesions or extra-nodal diseases; additionally, it is a daily, simple and helpful approach. Moreover, we describe the usefulness of cell blocks in the diagnosis of Hodgkin lymphoma.

Fine-needle aspiration for nucleic acid-ased molecular analyses in breast cancer

2009 ◽  
Vol 117 (1) ◽  
pp. 32-39 ◽  
Author(s):  
Catherine Uzan ◽  
Fabrice Andre ◽  
Veronique Scott ◽  
Isabelle Laurent ◽  
Elie Azria ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Nucleic Acid ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Molecular Analyses ◽  
Fine Needle
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