Fine-needle aspiration of thyroid tumors: Identifying factors associated with adequacy rate in a large academic center in the Netherlands

2010 ◽  
Vol 40 (S1) ◽  
pp. E21-E26 ◽  
Author(s):  
Sijbrigje G. A. de Meer ◽  
Jennifer M. J. Schreinemakers ◽  
Pierre M. J. Zelissen ◽  
Gerard Stapper ◽  
Daisy M. D. S. Sie-Go ◽  
...  
Keyword(s):  
The Netherlands ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Thyroid Tumors ◽  
Fine Needle ◽  
Factors Associated ◽  
Academic Center

A Rapid Method for DNA Extraction from Fine-Needle Aspiration Biopsies of Thyroid Tumors, and Subsequent RET Mutation Analysis

1998 ◽  
Vol 22 (6) ◽  
pp. 544-548 ◽  
Author(s):  
Jan Zedenius ◽  
Trisha Dwight ◽  
Bruce G. Robinson ◽  
Leigh Delbridge ◽  
Martin Backdahl ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Rapid Method ◽  
Mutation Analysis ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Thyroid Tumors ◽  
Fine Needle ◽  
Ret Mutation

scholarly journals Combined quantitation of HMGA2 mRNA, microRNAs, and mitochondrial-DNA content enables the identification and typing of thyroid tumors in fine-needle aspiration smears

BMC Cancer ◽  
2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Sergei E. Titov ◽  
Mikhail K. Ivanov ◽  
Pavel S. Demenkov ◽  
Gevork A. Katanyan ◽  
Eugenia S. Kozorezova ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Molecular Markers ◽  
Fine Needle Aspiration ◽  
Real Time Pcr ◽  
Thyroid Nodules ◽  
Nuclear Dna ◽  
Malignant Tumors ◽  
Needle Aspiration ◽  
Thyroid Tumors ◽  
Fine Needle

Abstract Background Analysis of molecular markers in addition to cytological analysis of fine-needle aspiration (FNA) samples is a promising way to improve the preoperative diagnosis of thyroid nodules. Nonetheless, in clinical practice, applications of existing diagnostic solutions based on the detection of somatic mutations or analysis of gene expression are limited by their high cost and difficulties with clinical interpretation. The aim of our work was to develop an algorithm for the differential diagnosis of thyroid nodules on the basis of a small set of molecular markers analyzed by real-time PCR. Methods A total of 494 preoperative FNA samples of thyroid goiters and tumors from 232 patients with known histological reports were analyzed: goiter, 105 samples (50 patients); follicular adenoma, 101 (48); follicular carcinoma, 43 (28); Hürthle cell carcinoma, 25 (11); papillary carcinoma, 121 (56); follicular variant of papillary carcinoma, 80 (32); and medullary carcinoma, 19 (12). Total nucleic acids extracted from dried FNA smears were analyzed for five somatic point mutations and two translocations typical of thyroid tumors as well as for relative concentrations of HMGA2 mRNA and 13 microRNAs and the ratio of mitochondrial to nuclear DNA by real-time PCR. A decision tree–based algorithm was built to discriminate benign and malignant tumors and to type the thyroid cancer. Leave-p-out cross-validation with five partitions was performed to estimate prediction quality. A comparison of two independent samples by quantitative traits was carried out via the Mann–Whitney U test. Results A minimum set of markers was selected (levels of HMGA2 mRNA and miR-375, − 221, and -146b in combination with the mitochondrial-to-nuclear DNA ratio) and yielded highly accurate discrimination (sensitivity = 0.97; positive predictive value = 0.98) between goiters with benign tumors and malignant tumors and accurate typing of papillary, medullary, and Hürthle cell carcinomas. The results support an alternative classification of follicular tumors, which differs from the histological one. Conclusions The study shows the feasibility of the preoperative differential diagnosis of thyroid nodules using a panel of several molecular markers by a simple PCR-based method. Combining markers of different types increases the accuracy of classification.

scholarly journals Vanishing Thyroid Tumors: A Diagnostic Dilemma After Ultrasonography-Guided Fine-Needle Aspiration

Thyroid ◽  
2013 ◽  
Vol 23 (2) ◽  
pp. 194-200 ◽  
Author(s):  
Ogechukwu P. Eze ◽  
Guoping Cai ◽  
Zubair W. Baloch ◽  
Ashraf Khan ◽  
Renu Virk ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Thyroid Tumors ◽  
Diagnostic Dilemma ◽  
Fine Needle

scholarly journals Progression from on-site to point-of-care fine needle aspiration service: Opportunities and challenges

CytoJournal ◽  
2010 ◽  
Vol 7 ◽  
pp. 6 ◽  
Author(s):  
Prabodh K Gupta
Keyword(s):  
Patient Care ◽  
Fine Needle Aspiration ◽  
Point Of Care ◽  
Standard Of Care ◽  
Needle Aspiration ◽  
Care Needs ◽  
Fine Needle ◽  
Laboratory Staff ◽  
Academic Center ◽  
Set Up

Background Standard-of-care requires the availability of an efficient, economical and accurate on-site fine needle aspiration (FNA) service. Presence of a trained individual during the procedure ensures an improved patient care. Appropriate selection of the equipment, interaction with the clinicians and compliance with the various regulations during the procedure is essential. This is often done by an on-site FNA service. Organization and implementation of such a system in a large academic center is challenging. Method we reviewed the ambulatory care needs in the new Perelman Center for Advanced Medicine (PeCAM). Multiple (9) FNA sites have been established keeping in view the patient's convenience, clinic demands, various regulatory requirements and laboratory staff. Each location has dedicated FNA station with microscopes and supplies. In addition, state- ofthe -art technologies including a mobile FNA cart (Penn-A- Cart), remote specimen evaluation (TeleCyP) have been incorporated. Results The new set up is extremely efficient and much valued by the patients and the clinicians. It has improved patient care. Conclusion With necessary investments and resources a point-of-care FNA service has been created which has improved patient care. This, albeit with certain modifications may serve as a model for FNA service.

Endoscopic ultrasound fine needle aspiration of solid pancreatic lesions in a Venezuelan academic center

2009 ◽  
Vol 69 (2) ◽  
pp. S234-S235
Author(s):  
Dervis Bandres ◽  
Olaya Brewer ◽  
Victor Bracho ◽  
Carolina Díaz ◽  
Victoria García ◽  
...  
Keyword(s):  
Endoscopic Ultrasound ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Fine Needle ◽  
Academic Center
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