scholarly journals Vanishing Thyroid Tumors: A Diagnostic Dilemma After Ultrasonography-Guided Fine-Needle Aspiration

Thyroid ◽  
2013 ◽  
Vol 23 (2) ◽  
pp. 194-200 ◽  
Author(s):  
Ogechukwu P. Eze ◽  
Guoping Cai ◽  
Zubair W. Baloch ◽  
Ashraf Khan ◽  
Renu Virk ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Thyroid Tumors ◽  
Diagnostic Dilemma ◽  
Fine Needle

A Rapid Method for DNA Extraction from Fine-Needle Aspiration Biopsies of Thyroid Tumors, and Subsequent RET Mutation Analysis

1998 ◽  
Vol 22 (6) ◽  
pp. 544-548 ◽  
Author(s):  
Jan Zedenius ◽  
Trisha Dwight ◽  
Bruce G. Robinson ◽  
Leigh Delbridge ◽  
Martin Backdahl ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Rapid Method ◽  
Mutation Analysis ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Thyroid Tumors ◽  
Fine Needle ◽  
Ret Mutation

scholarly journals A focal nodular Hürthle cell hyperplasia in Hashimoto′s thyroiditis: A diagnostic dilemma on fine needle aspiration

2014 ◽  
Vol 31 (4) ◽  
pp. 236
Author(s):  
ShirishS Chandanwale ◽  
TanmayiV Kulkarni ◽  
RuchirJ Patel ◽  
Dhaval Thakkar
Keyword(s):  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Cell Hyperplasia ◽  
Diagnostic Dilemma ◽  
Fine Needle ◽  
Hürthle Cell

scholarly journals Combined quantitation of HMGA2 mRNA, microRNAs, and mitochondrial-DNA content enables the identification and typing of thyroid tumors in fine-needle aspiration smears

BMC Cancer ◽  
2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Sergei E. Titov ◽  
Mikhail K. Ivanov ◽  
Pavel S. Demenkov ◽  
Gevork A. Katanyan ◽  
Eugenia S. Kozorezova ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Molecular Markers ◽  
Fine Needle Aspiration ◽  
Real Time Pcr ◽  
Thyroid Nodules ◽  
Nuclear Dna ◽  
Malignant Tumors ◽  
Needle Aspiration ◽  
Thyroid Tumors ◽  
Fine Needle

Abstract Background Analysis of molecular markers in addition to cytological analysis of fine-needle aspiration (FNA) samples is a promising way to improve the preoperative diagnosis of thyroid nodules. Nonetheless, in clinical practice, applications of existing diagnostic solutions based on the detection of somatic mutations or analysis of gene expression are limited by their high cost and difficulties with clinical interpretation. The aim of our work was to develop an algorithm for the differential diagnosis of thyroid nodules on the basis of a small set of molecular markers analyzed by real-time PCR. Methods A total of 494 preoperative FNA samples of thyroid goiters and tumors from 232 patients with known histological reports were analyzed: goiter, 105 samples (50 patients); follicular adenoma, 101 (48); follicular carcinoma, 43 (28); Hürthle cell carcinoma, 25 (11); papillary carcinoma, 121 (56); follicular variant of papillary carcinoma, 80 (32); and medullary carcinoma, 19 (12). Total nucleic acids extracted from dried FNA smears were analyzed for five somatic point mutations and two translocations typical of thyroid tumors as well as for relative concentrations of HMGA2 mRNA and 13 microRNAs and the ratio of mitochondrial to nuclear DNA by real-time PCR. A decision tree–based algorithm was built to discriminate benign and malignant tumors and to type the thyroid cancer. Leave-p-out cross-validation with five partitions was performed to estimate prediction quality. A comparison of two independent samples by quantitative traits was carried out via the Mann–Whitney U test. Results A minimum set of markers was selected (levels of HMGA2 mRNA and miR-375, − 221, and -146b in combination with the mitochondrial-to-nuclear DNA ratio) and yielded highly accurate discrimination (sensitivity = 0.97; positive predictive value = 0.98) between goiters with benign tumors and malignant tumors and accurate typing of papillary, medullary, and Hürthle cell carcinomas. The results support an alternative classification of follicular tumors, which differs from the histological one. Conclusions The study shows the feasibility of the preoperative differential diagnosis of thyroid nodules using a panel of several molecular markers by a simple PCR-based method. Combining markers of different types increases the accuracy of classification.

scholarly journals Vanishing Thyroid Nodules: A Rare Diagnostic Dilemma Induced by Ultrasound-Guided Fine-Needle Aspiration

2012 ◽  
Vol 138 (suppl 1) ◽  
pp. A206-A206
Author(s):  
Ogechukwu P. Eze ◽  
Guoping Cai ◽  
Zubair W. Baloch ◽  
Ashraf Khan ◽  
Lynwood W. Hammers ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Thyroid Nodules ◽  
Needle Aspiration ◽  
Ultrasound Guided ◽  
Diagnostic Dilemma ◽  
Fine Needle

scholarly journals Diagnostic difficulties and pitfalls in rapid on-site evaluation of endobronchial ultrasound guided fine needle aspiration

CytoJournal ◽  
2010 ◽  
Vol 7 ◽  
pp. 9 ◽  
Author(s):  
Sara E. Monaco* ◽  
Matthew J. Schuchert ◽  
Walid E. Khalbuss
Keyword(s):  
Fine Needle Aspiration ◽  
Final Diagnosis ◽  
Needle Aspiration ◽  
Endobronchial Ultrasound ◽  
Diagnostic Dilemma ◽  
Fine Needle ◽  
Preliminary Diagnosis ◽  
Diagnostic Difficulties ◽  
Site Evaluation ◽  
Background Material

Background: One of the novel techniques utilizing fine needle aspiration (FNA) in the diagnosis of mediastinal and lung lesions is the endobronchial ultrasound (EBUS)-guided FNA. In this study, we describe five cases which had a discrepancy between on-site evaluation and final diagnosis, or a diagnostic dilemma when rendering the preliminary diagnosis, in order to illustrate some of the diagnostic difficulties and pitfalls that can occur in EBUS FNA. Methods: A total of five EBUS FNA cases from five patients were identified in our records with a discrepancy between the rapid on-site evaluation (ROSE) and final diagnosis, or that addressed a diagnostic dilemma. All of the cases had histological confirmation or follow-up. The cytomorphology in the direct smears, cell block, and immunohistochemical stains were reviewed, along with the clinical history and other available information. Results: Two cases were identified with a nondefinitive diagnosis at ROSE that were later diagnosed as malignant (metastatic signet-ring cell adenocarcinoma and metastatic renal cell carcinoma (RCC)) on the final cytological diagnosis. Three additional cases were identified with a ROSE and final diagnosis of malignant (large cell neuroendocrine carcinoma (LCNEC) and two squamous cell carcinomas), but raised important diagnostic dilemmas. These cases highlight the importance of recognizing discohesive malignant cells and bland neoplasms on EBUS FNA, which may lead to a negative or a nondefinitive preliminary diagnosis. Neuroendocrine tumors can also be difficult due to the wide range of entities in the differential diagnosis, including benign lymphocytes, lymphomas, small and nonsmall cell carcinomas, and the lack of immunohistochemical stains at the time of ROSE. Finally, the background material in EBUS FNAs may be misleading and unrelated to the cells of interest. Conclusions: This study illustrates the cytomorphology of five EBUS FNA cases that address some of the diagnostic challenges witnessed while examining these specimens during ROSE. Many of the difficulties faced can be attributed to the baseline cellularity of the aspirates, the bronchial contamination, the difficulty identifying neoplasms with bland cytology, the wide spectrum of diseases that can occur in the mediastinum with overlapping cytomorphologic features, the mismatch between the background material and the cell populations present, and the overall unfamiliarity with these types of specimens.

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