Exact kinetic analysis of passive transport across a polarized confluent MDCK cell monolayer modeled as a single barrier

2004 ◽  
Vol 93 (8) ◽  
pp. 2108-2123 ◽  
Author(s):  
Thuy Thanh Tran ◽  
Tracy Gales ◽  
Beverly Maleeff ◽  
Tanya Aldinger ◽  
Joseph W. Polli ◽  
...  
Keyword(s):  
Kinetic Analysis ◽  
Mdck Cell ◽  
Cell Monolayer ◽  
Passive Transport

Synthesis and phosphorylation of cytoskeletal proteins during the in vitro biogenesis of MDCK cell monolayers

1989 ◽  
Vol 93 (1) ◽  
pp. 53-61
Author(s):  
G. Benitez-King ◽  
F. Cazares ◽  
I. Meza
Keyword(s):  
Amino Acid ◽  
Mdck Cell ◽  
Cell Shape ◽  
Cell Monolayer ◽  
Amino Acid Uptake ◽  
Culture Conditions ◽  
Cytoskeletal Proteins ◽  
Acid Uptake ◽  
Suspended Cells

In vitro formation of a functional MDCK cell monolayer requires the dynamic participation of the cytoskeleton. Cell shape, contacts and polarity, as well as transepithelial electric resistance (TER), are actively modified during this differentiation process. We studied the distribution and rearrangement of cytokeratin, vimentin and actin filaments that occur in the monolayer concomitant with the synthesis and phosphorylation of these proteins. Cells cultured for short time in suspension, subconfluent or early confluent cells, showed striking differences in cell shape and arrangement of their cytoskeletal filaments. Subconfluent and early confluent cells synthesized proteins at high levels. In contrast, suspended cells maintained lower rates of protein synthesis. Labeled amino acid uptake was very similar in all these culture conditions. Gel electrophoresis analyses of the synthesized proteins showed increases in the synthesis of actin, vimentin and specific cytokeratins in subconfluent and early confluent cells. On the other hand a decrease in total protein, actin, vimentin and cytokeratin synthesis was observed in cells kept in suspension for 24 h or in 78-h or 174-h older confluent cultures. These cultures also showed a decrease in the uptake of labeled amino acid. Cytokeratin and vimentin phosphorylation rates were also modified during the in vitro formation of a monolayer. In suspended cells, cytokeratins were phosphorylated and three labeled isoelectric variants of the 40, 48 and 58K (K = 10(3) Mr) cytokeratins were present in intermediate filament extracts. In subconfluent and early confluent cells only two isoelectric phosphorylated variants of the 40, 48 and 58K cytokeratins were detected and vimentin was also phosphorylated.(ABSTRACT TRUNCATED AT 250 WORDS)

Zinc ions regulate opening of tight junction favouring efflux of macromoleculesviathe GSK3β/snail-mediated pathway

Metallomics ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 169-179 ◽  
Author(s):  
Ruyue Xiao ◽  
Lan Yuan ◽  
Weijiang He ◽  
Xiaoda Yang
Keyword(s):  
Tight Junction ◽  
Mdck Cell ◽  
Cell Monolayer ◽  
Zinc Ions

Zn2+-Induced asymmetric paracellular pore paths in MDCK cell monolayer favour efflux of macromoleculesviathe GSK3β/snail-mediated pathway.

scholarly journals Transepithelial Transport and Metabolism of Boronated Dipeptides Across Caco-2 and HCT-8 Cell Monolayers

1996 ◽  
Vol 3 (6) ◽  
pp. 277-286
Author(s):  
Amy L. Elkins ◽  
John G. Eley ◽  
Merrill C. Miller III ◽  
Iris H. Hall ◽  
Anup Sood ◽  
...  
Keyword(s):  
Oral Delivery ◽  
Cell Monolayer ◽  
Therapeutic Agents ◽  
Transepithelial Transport ◽  
Passive Transport ◽  
Cell Monolayers ◽  
Proteins And Peptides

Oral delivery of proteins and peptides as therapeutic agents is problematic due to their low bioavailability. This study examined the effect of boronation on the transepithelial transport and metabolism of three glycine-phenylalanine dipeptides in Caco-2 and HCT-8 cell monolayers. The three dipeptides exhibited passive transport characteristics in the monolayer systems. However, metabolism of the boronated dipeptides did occur, but to a lesser extent than the non-boronated glycine-phenylalanine dipeptide. The same metabolic scheme was seen in both cell monolayer system, but greater metabolism was seen in the HCT-8 cell monolayers.

scholarly journals Penetration of Salmonella through a polarized Madin-Darby canine kidney epithelial cell monolayer.

1988 ◽  
Vol 107 (1) ◽  
pp. 221-230 ◽  
Author(s):  
B B Finlay ◽  
B Gumbiner ◽  
S Falkow
Keyword(s):  
Mdck Cell ◽  
Pathogenic Bacteria ◽  
Mdck Cells ◽  
Cell Monolayer ◽  
Apical Surface ◽  
Madin Darby Canine Kidney ◽  
Intracellular Parasites ◽  
Epithelial Barriers ◽  
Darby Canine Kidney ◽  
Canine Kidney

Many intracellular parasites are capable of penetrating host epithelial barriers. To study this process in more detail we examined the interactions between the pathogenic bacteria Salmonella choleraesuis and polarized epithelial monolayers of Madin-Darby canine kidney (MDCK) cells grown on membrane filters. Association of bacteria with the MDCK cell apical surface was an active event, requiring bacterial RNA and protein synthesis, and was blocked by low temperatures. Salmonella were internalized within a membrane-bound vacuole and exhibited penetration through, but not between MDCK cells. A maximum of 14 Salmonella per MDCK cell crossed the monolayer per hour to the basolateral surface yet the monolayer remained viable and impermeable to Escherichia coli. Apical S. choleraesuis infection resulted in an increase in paracellular permeability but the MDCK intercellular contacts were not significantly disrupted. Basolateral S. choleraesuis infection was inefficient, and only small numbers of S. choleraesuis penetrated to the apical medium.

The permeability and transport mechanism of graphene quantum dots (GQDs) across the biological barrier

Nanoscale ◽  
2015 ◽  
Vol 7 (5) ◽  
pp. 2034-2041 ◽  
Author(s):  
Xin-yi Wang ◽  
Rong Lei ◽  
Hong-duang Huang ◽  
Na Wang ◽  
Lan Yuan ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Transport Properties ◽  
Mdck Cell ◽  
Transport Mechanism ◽  
Cell Monolayer ◽  
Graphene Quantum Dots ◽  
Biological Barrier ◽  
Membrane Permeabilities

This work details the preparation of graphene quantum dots and investigates their membrane permeabilities and transport properties across the MDCK cell monolayer.

Monitoring the transport of polymeric micelles across MDCK cell monolayer and exploring related mechanisms

2012 ◽  
Vol 158 (3) ◽  
pp. 413-423 ◽  
Author(s):  
Shanshan Zhao ◽  
Wenbing Dai ◽  
Bing He ◽  
Jiancheng Wang ◽  
Zhonggui He ◽  
...  
Keyword(s):  
Mdck Cell ◽  
Polymeric Micelles ◽  
Cell Monolayer

scholarly journals FITC labeling of human insulin and transport of FITC-insulin conjugates through MDCK cell monolayer

2019 ◽  
Vol 9 (6) ◽  
pp. 400-405 ◽  
Author(s):  
Darshana Shah ◽  
Yuxing Guo ◽  
Joseph Ocando ◽  
Jun Shao
Keyword(s):  
Human Insulin ◽  
Mdck Cell ◽  
Cell Monolayer
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