Monitoring passive transport of redox mediators across a confluent cell monolayer with single-cell resolution by means of scanning electrochemical microscopy

2012 ◽  
Vol 4 (3) ◽  
pp. 623-629 ◽  
Author(s):  
Stefan Bergner ◽  
Joachim Wegener ◽  
Frank-Michael Matysik
Keyword(s):  
Single Cell ◽  
Cell Monolayer ◽  
Scanning Electrochemical Microscopy ◽  
Redox Mediators ◽  
Passive Transport ◽  
Confluent Cell ◽  
Electrochemical Microscopy

scholarly journals Characterization of Inherently Chiral Electrosynthesized Oligomeric Films by Voltammetry and Scanning Electrochemical Microscopy (SECM)

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5368
Author(s):  
Margherita Donnici ◽  
Rosanna Toniolo ◽  
Serena Arnaboldi ◽  
Patrizia R. Mussini ◽  
Tiziana Benincori ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Scanning Electrochemical Microscopy ◽  
Redox Mediators ◽  
Feedback Effects ◽  
Au Electrode ◽  
Competition Mode ◽  
Inherently Chiral ◽  
Discriminating Ability ◽  
Electrochemical Microscopy

A voltammetric and scanning electrochemical microscopy (SECM) investigation was performed on an inherently chiral oligomer-coated gold electrode to establish its general properties (i.e., conductivity and topography), as well as its ability to discriminate chiral electroactive probe molecules. The electroactive monomer (S)-2,2′-bis(2,2′-bithiophene-5-yl)-3,3′-bibenzothiophene ((S)-BT2T4) was employed as reagent to electrodeposit, by cyclic voltammetry, the inherently chiral oligomer film of (S)-BT2T4 (oligo-(S)-BT2T4) onto the Au electrode surface (resulting in oligo-(S)-BT2T4-Au). SECM measurements, performed in either feedback or competition mode, using the redox mediators [Fe(CN)6]4− and [Fe(CN)6]3− in aqueous solutions, and ferrocene (Fc), (S)-FcEA, (R)-FcEA and rac-FcEA (FcEA is N,N-dimethyl-1-ferrocenylethylamine) in CH3CN solutions, indicated that the oligomer film, as produced, was uncharged. The use of [Fe(CN)6]3− allowed establishing that the oligomer film behaved as a porous insulating membrane, presenting a rather rough surface. This was inferred from both the approach curves and linear and bidimensional SECM scans, which displayed negative feedback effects. The oligomer film acquired semiconducting or fully conducting properties when the Au electrode was biased at potential more positive than 0.6 V vs. Ag|AgCl|KCl. Under the latter conditions, the approach curves displayed positive feedback effects. SECM measurements, performed in competition mode, allowed verifying the discriminating ability of the oligo-(S)-BT2T4 film towards the (S)-FcEA and (R)-FcEA redox mediators, which confirmed the results obtained by cyclic voltammetry. SECM linear scans indicated that the enantiomeric discriminating ability of the oligo-(S)-BT2T4 was even across its entire surface.

Single Cell Viability Measured by Scanning Electrochemical Microscopy and Live/DeadTM Staining

2008 ◽  
Vol 14 (S2) ◽  
pp. 952-953 ◽  
Author(s):  
KM Jeerage ◽  
TL Oreskovic ◽  
N Goldstein ◽  
DS Lauria
Keyword(s):  
New Mexico ◽  
Cell Viability ◽  
Single Cell ◽  
Scanning Electrochemical Microscopy ◽  
Electrochemical Microscopy

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008

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