Permeation of vanadium(III, IV, V)-dipicolinate complexes across MDCK cell monolayer and comparison with Caco-2 cells

2005 ◽  
Vol 50 (17) ◽  
pp. 1854 ◽  
Author(s):  
Yue ZHANG
Keyword(s):  
Mdck Cell ◽  
Cell Monolayer

Exact kinetic analysis of passive transport across a polarized confluent MDCK cell monolayer modeled as a single barrier

2004 ◽  
Vol 93 (8) ◽  
pp. 2108-2123 ◽  
Author(s):  
Thuy Thanh Tran ◽  
Tracy Gales ◽  
Beverly Maleeff ◽  
Tanya Aldinger ◽  
Joseph W. Polli ◽  
...  
Keyword(s):  
Kinetic Analysis ◽  
Mdck Cell ◽  
Cell Monolayer ◽  
Passive Transport

Synthesis and phosphorylation of cytoskeletal proteins during the in vitro biogenesis of MDCK cell monolayers

1989 ◽  
Vol 93 (1) ◽  
pp. 53-61
Author(s):  
G. Benitez-King ◽  
F. Cazares ◽  
I. Meza
Keyword(s):  
Amino Acid ◽  
Mdck Cell ◽  
Cell Shape ◽  
Cell Monolayer ◽  
Amino Acid Uptake ◽  
Culture Conditions ◽  
Cytoskeletal Proteins ◽  
Acid Uptake ◽  
Suspended Cells

In vitro formation of a functional MDCK cell monolayer requires the dynamic participation of the cytoskeleton. Cell shape, contacts and polarity, as well as transepithelial electric resistance (TER), are actively modified during this differentiation process. We studied the distribution and rearrangement of cytokeratin, vimentin and actin filaments that occur in the monolayer concomitant with the synthesis and phosphorylation of these proteins. Cells cultured for short time in suspension, subconfluent or early confluent cells, showed striking differences in cell shape and arrangement of their cytoskeletal filaments. Subconfluent and early confluent cells synthesized proteins at high levels. In contrast, suspended cells maintained lower rates of protein synthesis. Labeled amino acid uptake was very similar in all these culture conditions. Gel electrophoresis analyses of the synthesized proteins showed increases in the synthesis of actin, vimentin and specific cytokeratins in subconfluent and early confluent cells. On the other hand a decrease in total protein, actin, vimentin and cytokeratin synthesis was observed in cells kept in suspension for 24 h or in 78-h or 174-h older confluent cultures. These cultures also showed a decrease in the uptake of labeled amino acid. Cytokeratin and vimentin phosphorylation rates were also modified during the in vitro formation of a monolayer. In suspended cells, cytokeratins were phosphorylated and three labeled isoelectric variants of the 40, 48 and 58K (K = 10(3) Mr) cytokeratins were present in intermediate filament extracts. In subconfluent and early confluent cells only two isoelectric phosphorylated variants of the 40, 48 and 58K cytokeratins were detected and vimentin was also phosphorylated.(ABSTRACT TRUNCATED AT 250 WORDS)

Zinc ions regulate opening of tight junction favouring efflux of macromoleculesviathe GSK3β/snail-mediated pathway

Metallomics ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 169-179 ◽  
Author(s):  
Ruyue Xiao ◽  
Lan Yuan ◽  
Weijiang He ◽  
Xiaoda Yang
Keyword(s):  
Tight Junction ◽  
Mdck Cell ◽  
Cell Monolayer ◽  
Zinc Ions

Zn2+-Induced asymmetric paracellular pore paths in MDCK cell monolayer favour efflux of macromoleculesviathe GSK3β/snail-mediated pathway.

scholarly journals Penetration of Salmonella through a polarized Madin-Darby canine kidney epithelial cell monolayer.

1988 ◽  
Vol 107 (1) ◽  
pp. 221-230 ◽  
Author(s):  
B B Finlay ◽  
B Gumbiner ◽  
S Falkow
Keyword(s):  
Mdck Cell ◽  
Pathogenic Bacteria ◽  
Mdck Cells ◽  
Cell Monolayer ◽  
Apical Surface ◽  
Madin Darby Canine Kidney ◽  
Intracellular Parasites ◽  
Epithelial Barriers ◽  
Darby Canine Kidney ◽  
Canine Kidney

Many intracellular parasites are capable of penetrating host epithelial barriers. To study this process in more detail we examined the interactions between the pathogenic bacteria Salmonella choleraesuis and polarized epithelial monolayers of Madin-Darby canine kidney (MDCK) cells grown on membrane filters. Association of bacteria with the MDCK cell apical surface was an active event, requiring bacterial RNA and protein synthesis, and was blocked by low temperatures. Salmonella were internalized within a membrane-bound vacuole and exhibited penetration through, but not between MDCK cells. A maximum of 14 Salmonella per MDCK cell crossed the monolayer per hour to the basolateral surface yet the monolayer remained viable and impermeable to Escherichia coli. Apical S. choleraesuis infection resulted in an increase in paracellular permeability but the MDCK intercellular contacts were not significantly disrupted. Basolateral S. choleraesuis infection was inefficient, and only small numbers of S. choleraesuis penetrated to the apical medium.

The permeability and transport mechanism of graphene quantum dots (GQDs) across the biological barrier

Nanoscale ◽  
2015 ◽  
Vol 7 (5) ◽  
pp. 2034-2041 ◽  
Author(s):  
Xin-yi Wang ◽  
Rong Lei ◽  
Hong-duang Huang ◽  
Na Wang ◽  
Lan Yuan ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Transport Properties ◽  
Mdck Cell ◽  
Transport Mechanism ◽  
Cell Monolayer ◽  
Graphene Quantum Dots ◽  
Biological Barrier ◽  
Membrane Permeabilities

This work details the preparation of graphene quantum dots and investigates their membrane permeabilities and transport properties across the MDCK cell monolayer.

Monitoring the transport of polymeric micelles across MDCK cell monolayer and exploring related mechanisms

2012 ◽  
Vol 158 (3) ◽  
pp. 413-423 ◽  
Author(s):  
Shanshan Zhao ◽  
Wenbing Dai ◽  
Bing He ◽  
Jiancheng Wang ◽  
Zhonggui He ◽  
...  
Keyword(s):  
Mdck Cell ◽  
Polymeric Micelles ◽  
Cell Monolayer

scholarly journals FITC labeling of human insulin and transport of FITC-insulin conjugates through MDCK cell monolayer

2019 ◽  
Vol 9 (6) ◽  
pp. 400-405 ◽  
Author(s):  
Darshana Shah ◽  
Yuxing Guo ◽  
Joseph Ocando ◽  
Jun Shao
Keyword(s):  
Human Insulin ◽  
Mdck Cell ◽  
Cell Monolayer

Nanoparticulate Formulations for Paclitaxel Delivery Across MDCK Cell Monolayer

2010 ◽  
Vol 16 (21) ◽  
pp. 2331-2340 ◽  
Author(s):  
Jingwei Xie ◽  
Chenlu Lei ◽  
Yong Hu ◽  
Gary Kaizhong Gay ◽  
Nazrul Hadi Bin Jamali ◽  
...  
Keyword(s):  
Mdck Cell ◽  
Cell Monolayer

Fine Structure of Cytochalasin Induced Polyploid Cells

1968 ◽  
Vol 26 ◽  
pp. 122-123
Author(s):  
Awtar Krishan ◽  
Nestor Bohonos
Keyword(s):  
Fine Structure ◽  
Cytochalasin B ◽  
Present Report ◽  
Cell Monolayer ◽  
Polyploid Cell ◽  
Specific Cell ◽  
Nuclear Surface ◽  
Unsuccessful Attempt ◽  
Daughter Cells ◽  
Polyploid Cells

Cytochalasin B, a mould metabolite from Helminthosporium dermatioideum has been shown to interfere with specific cell activities such as cytoplasmic cleavage and cell movement. Cells undergoing nuclear division in the presence of cytochalasin B are unable to complete the separation of the resulting daughter cells. In time-lapse studies, the daughter cells coalesce after an initial unsuccessful attempt at separation and form large multinucleate polyploid cells. The present report describes the fine structure of the large polyploid cells induced in Earle's L-cell monolayer cultures by exposure to cytochalasin B (lγ/ml) for 92 hours.In the present material we have seen as many as 7 nuclei in these polyploid cells. Treatment with cytochalasin B for longer periods of time (6 to 7 days, with one medium change on the 3rd day) did not increase the number of nuclei beyond the 7 nuclei stage. Figure 1 shows a large polyploid cell with four nuclei. These nuclei are indistinguishable in their fine structure from those of the cells from control cultures but often show unusually large numbers of cytoplasmic invaginations and extensions of the nuclear surface (Figure 2).

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