scholarly journals Transepithelial Transport and Metabolism of Boronated Dipeptides Across Caco-2 and HCT-8 Cell Monolayers

1996 ◽  
Vol 3 (6) ◽  
pp. 277-286
Author(s):  
Amy L. Elkins ◽  
John G. Eley ◽  
Merrill C. Miller III ◽  
Iris H. Hall ◽  
Anup Sood ◽  
...  
Keyword(s):  
Oral Delivery ◽  
Cell Monolayer ◽  
Therapeutic Agents ◽  
Transepithelial Transport ◽  
Passive Transport ◽  
Cell Monolayers ◽  
Proteins And Peptides

Oral delivery of proteins and peptides as therapeutic agents is problematic due to their low bioavailability. This study examined the effect of boronation on the transepithelial transport and metabolism of three glycine-phenylalanine dipeptides in Caco-2 and HCT-8 cell monolayers. The three dipeptides exhibited passive transport characteristics in the monolayer systems. However, metabolism of the boronated dipeptides did occur, but to a lesser extent than the non-boronated glycine-phenylalanine dipeptide. The same metabolic scheme was seen in both cell monolayer system, but greater metabolism was seen in the HCT-8 cell monolayers.

Transepithelial transport mechanisms of 7,8-dihydroxyflavone, a small molecular TrkB receptor agonist, in human intestinal Caco-2 cells

2019 ◽  
Vol 10 (8) ◽  
pp. 5215-5227 ◽  
Author(s):  
Yufeng Chen ◽  
Fan Xue ◽  
Guobin Xia ◽  
Zhenlei Zhao ◽  
Chun Chen ◽  
...  
Keyword(s):  
Receptor Agonist ◽  
Transepithelial Transport ◽  
Efflux Transporters ◽  
Transport Mechanisms ◽  
Passive Transport ◽  
Cell Monolayers ◽  
Trkb Receptor

7,8-DHF was transported across Caco-2 cell monolayers by passive transport with carrier-mediated influx and efflux transporters.

scholarly journals Intestinal Transportations of Main Chemical Compositions of Polygoni Multiflori Radix in Caco-2 Cell Model

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Jie Yu ◽  
Na Li ◽  
Pei Lin ◽  
Yunfei Li ◽  
Xiaojian Mao ◽  
...  
Keyword(s):  
Gibbs Energy ◽  
Cell Model ◽  
Cell Monolayer ◽  
Intestinal Transport ◽  
Transepithelial Transport ◽  
Chemical Compositions ◽  
Polygonum Multiflorum ◽  
Close Attention ◽  
Apparent Permeability ◽  
Cell Monolayers

Context. Polygoni Multiflori Radix (PMR) is originated from the root ofPolygonum multiflorumThunb. and used in oriental countries for centuries. However, little researches pay close attention to the absorption of its major constituents.Objective. Transepithelial transport of TSG, RL, PL, and four anthraquinones is carried out.Materials and Methods. Caco-2 cell monolayer, which represented a well-established model for the study of intestinal transport of nutrients and xenobiotics, was used in this paper.Results. The apparent permeability coefficients (Papp) in the Caco-2 cell monolayers were TSG (2.372 × 10−9) < EG (2.391 × 10−9) < EN (2.483 × 10−9) < PL (4.917 × 10−9) < RN (1.707 × 10−8) < RL (1.778 × 10−8) < AE (1.952 × 10−8). Thus, RN, RL, and AE were considered partly absorbed, while other constituents were hardly absorbed.Discussion and Conclusion. Glycosides showed poor permeabilities than aglycones. In the meantime, TSG and EN gave out poor recovery rates in this assay, which indicated that TSG and EN may accumulate or metabolise in the Caco-2 cells.In silicoprediction indicated that Gibbs energy (r=0.751,p<0.05) and heat of form (r=0.701,p<0.05) were strongly positively correlated withPapp.

Processing Immunoperoxidase Labeled Cell Monolayers and Cryostat Sesctions For Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 714-715
Author(s):  
K. Chien ◽  
R. Van de Velde ◽  
I.P. Shintaku ◽  
A.F. Sassoon
Keyword(s):  
Cell Monolayer ◽  
Cell Types ◽  
Surface Antigens ◽  
Immunoperoxidase Method ◽  
Reaction Step ◽  
Hot Plate ◽  
Air Drying ◽  
New Approach ◽  
Cell Monolayers ◽  
Glass Slides

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.

Copper repletion enhances apical iron uptake and transepithelial iron transport by Caco-2 cells

2002 ◽  
Vol 282 (3) ◽  
pp. G527-G533 ◽  
Author(s):  
Okhee Han ◽  
Marianne Wessling-Resnick
Keyword(s):  
Iron Uptake ◽  
Iron Transport ◽  
Cell Monolayer ◽  
Transepithelial Transport ◽  
Apical Surface ◽  
Divalent Metal Transporter 1 ◽  
Divalent Metal Transporter ◽  
Metal Transporter ◽  
Copper Status ◽  
Copper Supplementation

The influence of copper status on Caco-2 cell apical iron uptake and transepithelial transport was examined. Cells grown for 7–8 days in media supplemented with 1 μM CuCl2had 10-fold higher cellular levels of copper compared with control. Copper supplementation did not affect the integrity of differentiated Caco-2 cell monolayers grown on microporous membranes. Copper-repleted cells displayed increased uptake of iron as well as increased transport of iron across the cell monolayer. Northern blot analysis revealed that expression of the apical iron transporter divalent metal transporter-1 (DMT1), the basolateral transporter ferroportin-1 (Fpn1), and the putative ferroxidase hephaestin (Heph) was upregulated by copper supplementation, whereas the recently identified ferrireductase duodenal cytochrome b (Dcytb) was not. These results suggest that DMT1, Fpn1, and Heph are involved in the iron uptake process modulated by copper status. Although a clear role for Dcytb was not identified, an apical surface ferrireductase was modulated by copper status, suggesting that its function also contributes to the enhanced iron uptake by copper-repleted cells. A model is proposed wherein copper promotes iron depletion of intestinal Caco-2 cells, creating a deficiency state that induces upregulation of iron transport factors.

Multivesicular liposome: A lipid-based drug delivery system for efficient drug delivery

2021 ◽  
Vol 27 ◽  
Author(s):  
Bapi Gorain ◽  
Bandar E. Al-Dhubiab ◽  
Anroop Nair ◽  
Prashant Kesharwani ◽  
Manisha Pandey ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Sustained Release ◽  
Delivery System ◽  
Targeted Delivery ◽  
Drug Loading ◽  
Therapeutic Agents ◽  
Laboratory Research ◽  
Efficacy And Safety ◽  
Liposomal Delivery ◽  
Proteins And Peptides

: The advancement of delivery tools for therapeutic agents has brought several novel formulations with increased drug loading, sustained release, targeted delivery, and prolonged efficacy. Amongst the several novel delivery approaches, multivesicular liposome has gained potential interest because this delivery system possesses the above advantages. In addition, this multivesicular liposomal delivery prevents degradation of the entrapped drug within the physiological environment while administered. The special structure of the vesicles allowed successful entrapment of hydrophobic and hydrophilic therapeutic agents, including proteins and peptides. Furthermore, this novel formulation could maintain the desired drug concentration in the plasma for a prolonged period, which helps to reduce the dosing frequencies, improve bioavailability, and safety. This tool could also provide stability of the formulation, and finally gaining patient compliance. Several multivesicular liposomes received approval for clinical research, while others are at different stages of laboratory research. In this review, we have focused on the preparation of multivesicular liposomes along with their application in different ailments for the improvement of the performance of the entrapped drug. Moreover, the challenges of delivering multivesicular vesicles have also been emphasized. Overall, it could be inferred that multivesicular liposomal delivery is a novel platform of advanced drug delivery with improved efficacy and safety.

Cell Monolayer Deformation Microscopy reveals mechanical fragility of cell monolayers following EMT

2022 ◽  
Author(s):  
Amy A. Sutton ◽  
Clayton W. Molter ◽  
Ali Amini ◽  
Johanan Idicula ◽  
Max Furman ◽  
...  
Keyword(s):  
Cell Monolayer ◽  
Cell Monolayers

Exact kinetic analysis of passive transport across a polarized confluent MDCK cell monolayer modeled as a single barrier

2004 ◽  
Vol 93 (8) ◽  
pp. 2108-2123 ◽  
Author(s):  
Thuy Thanh Tran ◽  
Tracy Gales ◽  
Beverly Maleeff ◽  
Tanya Aldinger ◽  
Joseph W. Polli ◽  
...  
Keyword(s):  
Kinetic Analysis ◽  
Mdck Cell ◽  
Cell Monolayer ◽  
Passive Transport

Recent insights in the use of nanocarriers for the oral delivery of bioactive proteins and peptides

Peptides ◽  
2018 ◽  
Vol 101 ◽  
pp. 112-123 ◽  
Author(s):  
Patrícia Batista ◽  
Pedro M. Castro ◽  
Ana Raquel Madureira ◽  
Bruno Sarmento ◽  
Manuela Pintado
Keyword(s):  
Oral Delivery ◽  
Bioactive Proteins ◽  
Proteins And Peptides
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